Use of portable microbial samplers for estimating inhalation exposure to viable biological agents

Yao, Maosheng; Mainelis, Gediminas

doi:10.1038/sj.jes.7500517

Cited by 31 publications

(21 citation statements)

References 47 publications

(30 reference statements)

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“…In a previous study, an average diameter of 0.73 lm was used both for B. subtilis var niger and for P. fluorescens (Mainelis et al 2001). In this work, average diameters for both B. subtilis var niger (0.8 lm) and P. fluorescens (0.6 lm) in reference to a previous study (Yao and Mainelis 2007) were also used for the charge calculation. As indicated in Fig.…”

Section: Resultsmentioning

confidence: 98%

A novel method for measuring the charge distribution of airborne microbes

2010

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A novel method for measuring bioaerosol charge distribution was investigated using electrostatic sampling and quantitative polymerase chain reaction (qPCR). Here, Bacillus subtilis var niger and Pseudomonas fluorescens were aerosolized and precipitated into different regions of two 96-well plates placed inside an electrostatic sampler. The concentrations of negatively charged bacteria in the air samples collected were quantified using qPCR, and the relevant bioaerosol charge levels were calculated using an equation developed in this study. Experimental results showed that B. subtilis var niger bioaerosols had wider charge distribution than P. fluorescens. For B. subtilis var niger, 93% of them carried a charge level of 13-42 units, 2% carried a charge level of 8-13 or lower, and 5% carried a charge level of 42-195 or higher. While for P. fluorescens, 99% of P. fluorescens aerosols carried a charge level of 14-34 elementary units. The indicative charge levels of bioaerosols collected at a certain point in the electrostatic sampling line largely depends on the particle size, bacterial species, electrostatic field strength, and the sampling flow rates for a given electrostatic sampler. In general, the trends for the charge levels here were similar to those obtained in previous studies. Use of qPCR technology in this study can distinguish between biological and non-biological particles collected. Thus, this avoided counting the non-biological particles in traditional bioaerosol charge measurements in which an optical particle counter is used. The developed technique here can be also used to analyze the charge levels for other types of bioaerosols.

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Section: Resultsmentioning

confidence: 98%

A novel method for measuring the charge distribution of airborne microbes

2010

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“…No. 30709), which is known as an effective instrument for bioaerosol sampling and is widely used around the world (Yao and Mainelis. 2007;Reponen et al, 2001;Saldanha and Manno, 2008).…”

Section: Culturable Fungimentioning

confidence: 99%

Airborne culturable fungi in naturally ventilated primary school environments in a subtropical climate

Salonen

Duchaine

Mazaheri

et al. 2015

Atmospheric Environment

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“…Such devices do not require heavy external pumps and feature high sampling flow rates. Various performance parameters of a series of portable impactors have been compared when collecting polystyrene latex particles and biological particles under controlled laboratory conditions [43][44][45]. Results suggested that when impactors are used for the collection of airborne bacteria and fungi, sampling times should be kept as short as possible to minimize under-representation of airborne microorganism concentration.…”

Section: Collection Of Air Samplesmentioning

confidence: 99%

Bioaerosols in Indoor Environment - A Review with Special Reference to Residential and Occupational Locations

Brandl¹

2011

TOEBMJ

159

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Bioaerosols such as bacterial and fungal cells and their spores are -along with non-biological particles -part of indoor airborne particulate matter and have been related since a long time to health issues of human beings as well as flora, and fauna. To identify the different risks and to establish exposure thresholds, microbiology of air samples from a series of indoor environments must be characterized, i.e. the different microorganisms (bacteria and fungi) must be identified and quantified. This review discusses the techniques of air sampling and sample analysis. In addition, a literature study has been performed regarding the levels of these microorganisms in various indoor occupational (e.g., schools, offices, hospitals, museums) and dwelling environments. These results will provide a significant scientific basis for indoor air quality control and help in elaborating risk prevention programs for workers and dwellers. This review shall contribute to the knowledge of identification and quantification of airborne microbial constituents in various indoor environments. Combining the indoor microbial load data with data from studies focusing on health effects caused by inhalation of specific airborne microorganisms will allow the evaluation of various risks to which inhabitants are exposed. Bioaerosols in Indoor Environment -A Review with Special Reference to Residential and Occupational LocationsJyotshna Mandal and Helmut Brandl* Institute of Evolutionary Biology and Environmental Studies, University of Zurich, SwitzerlandAbstract: Bioaerosols such as bacterial and fungal cells and their spores are -along with non-biological particles -part of indoor airborne particulate matter and have been related since a long time to health issues of human beings as well as flora, and fauna. To identify the different risks and to establish exposure thresholds, microbiology of air samples from a series of indoor environments must be characterized, i.e. the different microorganisms (bacteria and fungi) must be identified and quantified. This review discusses the techniques of air sampling and sample analysis. In addition, a literature study has been performed regarding the levels of these microorganisms in various indoor occupational (e.g., schools, offices, hospitals, museums) and dwelling environments. These results will provide a significant scientific basis for indoor air quality control and help in elaborating risk prevention programs for workers and dwellers. This review shall contribute to the knowledge of identification and quantification of airborne microbial constituents in various indoor environments. Combining the indoor microbial load data with data from studies focusing on health effects caused by inhalation of specific airborne microorganisms will allow the evaluation of various risks to which inhabitants are exposed.

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Use of portable microbial samplers for estimating inhalation exposure to viable biological agents

Cited by 31 publications

References 47 publications

A novel method for measuring the charge distribution of airborne microbes

A novel method for measuring the charge distribution of airborne microbes

Airborne culturable fungi in naturally ventilated primary school environments in a subtropical climate

Bioaerosols in Indoor Environment - A Review with Special Reference to Residential and Occupational Locations

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