Use of Polymerase Chain Reaction for Bivalve Pathogen Surveillance in the Yellow Clam <i>Mesodesma mactroides</i>

Carvalho, Yuri Bovi Morais; Santos, Juan Jethro da Silva; Raibenberg, Fernando C.; Poersch, Luís; Romano, Luís Alberto

doi:10.1080/08997659.2016.1152324

Cited by 5 publications

(3 citation statements)

References 13 publications

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“…In this study, although OsHV-1 was not detected in any of the tested yellow clams during the mortality event, the possibility that some other viral agent is implicated cannot be ruled out. Similar results were reported recently by Carvalho et al (2016), where screening in A. mactroides showed no evidence of the specific sequences of OsHV-1 m-var.…”

Section: Main Characteristics and Histological Examination Ofsupporting

confidence: 91%

Mass Mortalities Affecting Populations of the Yellow ClamAmarilladesma mactroidesAlong Its Geographic Range

Vázquez

Fiori²,

Arzul

et al. 2016

Journal of Shellfish Research

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The yellow clam Amarilladesma mactroides (Deshayes, 1854) is considered a vulnerable species since the mid-1990s. Populations have experienced mass mortalities throughout their entire range of distribution (23-41°S) along exposed sandy beaches of Atlantic South America. Detrimental anthropogenic impacts have further contributed to failure of populations to make a recovery. To determine the factors involved in these events, density prior to a mortality event was calculated and live yellow clams encompassing most of its geographic range distribution were analyzed histologically to describe parasites and pathologies. Moreover, moribund specimens were analyzed by molecular techniques to test for the presence of the virus OsHV-1. A mortality event was recorded after a maximum density of 127 clams/m 2 was attained. No clear pattern was found between the prevalence and intensity of infection and localities, mortality events, or sampling season. Although OsHV-1 was not observed in any of the yellow clams tested, the possibility that another viral agent was implicated cannot be ruled out. The presence of bacteria of the genus Vibrio in combination with stress caused by a relatively high population density is suggested as the likely cause of these episodic mass mortalities.

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Section: Main Characteristics and Histological Examination Ofsupporting

confidence: 91%

Mass Mortalities Affecting Populations of the Yellow ClamAmarilladesma mactroidesAlong Its Geographic Range

Vázquez

Fiori²,

Arzul

et al. 2016

Journal of Shellfish Research

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“…Intracytoplasmic vacuoles have been observed in all types of epithelial cells from distinct tissues, although they are most commonly observed in the gills (Meyers, 1981; Gulka et al, 1983; Leibovitz et al, 1984; Norton et al, 1992; Renault & Cochennec, 1994; Villalba et al, 1999; Meyers & Burton, 2009; Zhu et al, 2012; Carvalho et al, 2013; Da Silva et al, 2015), digestive gland (Harshbarger & Chang, 1977; Gulka et al, 1983; Comps, 1986; Wen et al, 1994; Diggles & Oliver, 2005; Lohrmann, 2009; Cáceres‐Martínez et al, 2010; Boehs et al, 2010; Montenegro et al, 2012; Cáceres‐Martínez & Vásquez‐Yeomans, 2013; Carvalho et al, 2016) and mantle (Meyers, 1981; Norton et al, 1992, 1993b; Wu & Pan, 1999a; Wu & Pan, 2000; Sun & Wu, 2004; Meyers & Burton, 2009; Zhu et al, 2012). In rare cases, IVs have been reported in the gonads (Fries & Grant, 1991; Cremonte et al, 2005).…”

Section: Histologymentioning

confidence: 99%

Rickettsiales‐like organisms in bivalves and marine gastropods: a review

Cruz‐Flores

Cáceres-Martı́nez

2020

Reviews in Aquaculture

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Rickettsiales‐like organisms (RLOs) are intracellular bacteria that have been reported to be symbionts of over 60 species of bivalves and marine gastropod molluscs of aquaculture importance around the world. Morphologically, some of these prokaryotes are similar to bacteria from the order Rickettsiales, and in most cases, they form intracytoplasmic inclusions similar to those observed in bacteria of the family Anaplasmataceae. Genetically, only Candidatus Xenohaliotis californiensis (CXc), a parasite of several abalone species, has been classified as a member of the order Rickettsiales. The other two characterized RLOs are distantly related; one belongs to the same class (α‐proteobacteria), and the other belongs to the γ‐proteobacteria. Hyperparasitism of RLOs by bacteriophages has been reported in several species of mollusc and appears to have a negative effect on RLOs. The intracellular lifestyle of RLOs has been associated with cell alterations including hypertrophy and compression of the cytoplasmic organelles, and in most cases, no host reaction is observed by histology. In some cases, a high infection intensity of RLOs has been related to dysfunction of the affected organs that can result in disease, and in other cases, high burdens of RLOs have been associated with mortality, but most of these cases have yet to be confirmed. To date, only CXc has been confirmed as the aetiological agent of a chronic and potentially lethal disease of several abalone species known as withering syndrome. This disease is listed by the World Organization of Animal Health. With the ever‐increasing interest in preserving fisheries and expanding the aquaculture production of mollusc species, more efforts should be focused on the characterization and study of RLOs to elucidate the type of symbiosis they maintain with their host and determine when they are acting as true pathogens that should be subjected to control in aquaculture. This review provides an overview of the literature on RLOs that infect bivalves and gastropods of aquaculture importance, and we discuss the most important aspects of these unique prokaryotes.

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“…Mesodesma mactroides) is a native surf clam from southernmost Brazil, Uruguay and Argentina. This species has recently been the subject of studies (Santos et al 2016;Carvalho et al 2016;Santos et al 2020a;Santos et al 2020b) and stands out for its historical value as a significant fishing resource on the mentioned countries (Coscarón 1959).…”

mentioning

confidence: 99%

Presence of amyloid protein in the yellow clam Amarilladesma mactroides (Reeve, 1854), an emerging species for aquaculture

Gomes

Romano

2021

AquaTechnica

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Las proteínas amiloides son grupos proteicos que comprenden una superfamilia de apolipoproteínas altamente conservadas en vertebrados, estando implicadas en la modulación de numerosas respuestas inmunes durante infecciones, lesiones o estrés. En este estudio, la almeja amarilla Amarilladesma mactroides se utilizó como modelo para invertebrados y demostrar depósitos de proteína amiloide en sus tejidos. Se recolectaron de la playa ejemplares adultos de este bivalvo y se transportaron al Laboratorio de Inmunología y Patología de Organismos Acuáticos (LIPOA) de la Universidad Federal de Rio Grande (FURG). Sus tejidos se procesaron para su estudio histológico y se tiñeron con hematoxilina y eosina, y Rojo Congo. Las secciones histológicas teñidas con hematoxilina y eosina se examinaron con microscopía óptica clásica. Además, las secciones fueron teñidas con Rojo Congo y se analizaron con microscopía de luz polarizada. La tinción con Rojo Congo reveló la presencia de proteínas amiloides en el pie muscular y en las glándulas digestivas de la almeja amarilla. Las técnicas utilizadas demostraron ser adecuadas para la determinación de proteína amiloide en esta especie y evidenciaron un papel complementario de las proteínas amiloides en la respuesta inmune, indicando que pueden servir como marcador del sistema inmunológico innato y la salud de los invertebrados. La capacidad de evaluar la condición inmunológica puede proporcionar información relacionada con el cultivo de especies emergentes para la acuicultura.

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Use of Polymerase Chain Reaction for Bivalve Pathogen Surveillance in the Yellow Clam Mesodesma mactroides

Cited by 5 publications

References 13 publications

Mass Mortalities Affecting Populations of the Yellow ClamAmarilladesma mactroidesAlong Its Geographic Range

Mass Mortalities Affecting Populations of the Yellow ClamAmarilladesma mactroidesAlong Its Geographic Range

Rickettsiales‐like organisms in bivalves and marine gastropods: a review

Presence of amyloid protein in the yellow clam Amarilladesma mactroides (Reeve, 1854), an emerging species for aquaculture

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