Use of polylysine-coated slides in preparation of cell samples for diagnostic cytology with special reference to urine sample.

Abstract

After some years of seeking a simple but effective method to lay cells on slides in an evenly dispersed monolayer for automated cell scanners we managed to utilise what we believe to be the electrical charges on the cell and slide surface to achieve this end. The machine scanners required additional techniques to disrupt cell clusters and prevent reaggregation of cells, and these are detailed in our previous publication.' These are not necessary for visual screening.It is well known that cells acquire a negative charge in solution, and we sought to increase this on the one hand and, on the other, to induce a positive charge on the slide surface. After much experimentation we achieved some success with the latter by the use of the cationic polymerised amino acid polylysine, which attaches as a monolayer to the negatively charged glass surface, leaving excess positive charge available for the attraction of cells.2 The latter, moreover, appear to remain discrete, if not to repel each other under gentle sedimentation forces of 1 g, to result in a fairly well-dispersed monolayer. Whatever the form of attachment achieved, it appears strong enough to withstand the gentle 'wash-off' of the fluid after 2-5 minutes' sedimentation and the rigours of the subsequent staining schedule.We thought that such a technique would be useful in a number of different ways in routine cytology practice where cells are in solution to start off with, as in the case of urine, or where, to produce better results, cells could be put into suspension-not necessarily dispersed-and then laid down more evenly and flat with less curling or overlap of the cytoplasm or even nucleus. We have found that cyst fluids lend themselves readily to such techniques, which result in more even cell spreads without the accompanying debris, and this aspect has been

Optimizing the DNA yield for molecular analysis from cytologic preparations

“…These slides are widely used, especially when adequate cellularity is not an issue and ancillary studies, photographing, or both are anticipated. The PC slides have a specialized surface that electrostatically enhances the adhesion of cellular material, which is negatively charged at a physiological pH . In our experience, the cellular adhesion is inferior to that of FF slides (data not shown), and the slides are more expensive than NF slides.…”

“…The PC slides have a specialized surface that electrostatically enhances the adhesion of cellular material, which is negatively charged at a physiological pH. 30,31 In our experience, the cellular adhesion is inferior to that of FF slides (data not shown), and the slides are more expensive than NF slides. The SC slide 32 allows covalent bonding of cells to the slide surface, which increases cellular adhesion, and it is used in our cytology laboratory primarily for fat-pad aspirations where the aspirate is allowed to air-dry.…”

Terpenes and cannabidiol against human corona and influenza viruses–Anti-inflammatory and antiviral in vitro evaluation

“…MRC-5 cells were plated on one 96-well plate and on two 96-well plates with polylysine-coated 0.5-mm coverslips, as previously described [25] in their culture medium, at 1 × 10 4 cells/well. Cells were allowed to attach for 16–24 h at 37 °C, in 5 % CO 2 .…”

Slide Preparation

“…In 1980, 12 Husain wrote: "After some years of seeking a simple but effective method to lay cells on slides in an evenly dispersed monolayer for automated cell scanners, we managed to utilise what we believe to be the electrical charges on the cell and slide surfaces to achieve this end. The machine scanners required additional techniques to disrupt cell clusters and prevent reaggregation of the cells… These are not necessary for visual screening."…”