Use of Platelet-rich fibrin as an autologous biologic rejuvenating media for avulsed teeth - an<i>in vitro</i>study

Hiremath, Hemalatha; Kulkarni, Suhas; Sharma, Robin; Hiremath, Vishwanath; Motiwala, Tejas

doi:10.1111/edt.12119

Cited by 6 publications

(14 citation statements)

References 30 publications

(37 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Of these, four studies exclusively recommended HBSS, while 12 studies recommended HBSS along with other media . Specialized media were also recommended in some studies, while saline and its variants were specifically recommended in four studies . There was only one recommendation for saliva .…”

Section: Resultsmentioning

confidence: 99%

“…The risk of bias in studies was also assessed and is depicted in Figure . In regard to randomization, there was no mention of random sequence generation in any study; while 33% of the studies mentioned random allocation of samples . Where only abstracts could be retrieved, the risk of selective reporting was unclear .…”

Section: Resultsmentioning

confidence: 99%

“…[36][37][38] Twentyone studies recommended milk in some form in addition to the other media tested, while 13 of these studies exclusively recommended milk. 39 Specialized media were also recommended in some studies, [67][68][69][70][71][72][73] while saline and its variants were specifically recommended in four studies. [74][75][76][77] There was only one recommendation for saliva.…”

Section: Resultsmentioning

confidence: 99%

“…In regard to randomization, there was no mention of random sequence generation in any study; while 33% of the studies mentioned random allocation of samples. 12,15,16,18,19,21,23,24,27,28,32,34,53,54,58,[62][63][64]71,72,74,75 Where only abstracts could be retrieved, the risk of selective reporting was unclear. 39,40,43,44,67 However, no such bias could be detected in the full-text articles.…”

Section: Resultsmentioning

confidence: 99%

“…Ideally, the most recommended storage medium should be able to not only maintain PDL cell viability but also enhance it. However, this enhancement of PDL cell viability was only observed in a few studies . The actual number of cells that remained viable after being placed in the storage medium could not be compared in the selected studies because of the great variability in the methodology of studies.…”

Section: Discussionmentioning

confidence: 99%

See 4 more Smart Citations

Which is the most recommended medium for the storage and transport of avulsed teeth? A systematic review

Adnan

Lone

Khan

et al. 2018

Dental Traumatology

105

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Which is the most recommended medium for the storage and transport of avulsed teeth? A systematic review

Adnan

Lone

Khan

et al. 2018

Dental Traumatology

105

View full text Add to dashboard Cite

show abstract

Assessment of platelet-rich fibrin in the maintenance and recovery of cell viability of the periodontal ligament

Navarro¹,

Barchiki²,

NavarroÂ³

et al. 2019

Sci Rep

View full text Add to dashboard Cite

This study analyzed the efficacy of autologous platelet-rich fibrin (PRF) in maintaining and recovering cell viability of the periodontal ligament (PDL). The PDL cells were isolated from 45 extracted teeth randomly distributed among 6 groups: 5 min, 1 h, 2 h, PRF 30 min, PRF 1 h and PRF 2 h. In the groups 5 min, 1 h and 2 h (n = 5), the teeth were kept dry in extra-alveolar times of 5 min, 1 h and 2 h respectively. The teeth of the groups PRF 30 min, PRF 1 h and PRF 2 h (n = 10) were kept dry at extra-alveolar times of 30 min, 1 and 2 h followed by immersion in PRF for 45 min. PDL cells were isolated by enzymatic digestion with type II collagenase and dispase, counted and analyzed for viability with Trypan blue vital dye in Neubauer chamber. The variables total number of cells and cell viability demonstrated that in the 5 min, 1 h and 2 h groups there was a decrease after the extra-alveolar dry times of 1 and 2 h. In comparison with the total number of cells, group 1 h, considered immediate reimplantation, did not present statistical difference when compared to the groups PRF 30 min, PRF 1 h and 2 h, a result that demonstrates that PRF assists in cell maintenance and recovery. PRF provided increased cell viability in relation to the different dry extra-alveolar times analyzed (p < 0.001). Autologous PRF presented effectiveness in maintaining and recovering PDL cells from extracted teeth and kept dry for up to 2 h.

show abstract

Rescuing “hopeless” avulsed teeth using autologous platelet-rich fibrin following delayed reimplantation: Two case reports

Yang¹,

Li²,

Jia³

et al. 2023

World J Clin Cases

View full text Add to dashboard Cite

BACKGROUND Tooth avulsion is one of the most severe types of dental trauma. Most avulsed teeth undergo long-term ankylosis and replacement resorption after delayed reimplantation and exhibit a poor prognosis. The aim of this work was to improve the success rate of avulsed teeth after delayed reimplantation using autologous platelet-rich fibrin (PRF). CASE SUMMARY Case 1 was a 14-year-old boy who fell and knocked out his left upper central incisor 18 h prior to his arrival at the department. The diagnoses were avulsion of tooth 21, lateral luxation of tooth 11 and alveolar fracture of teeth 11 and 21. In case 2, a 17-year-old boy fell 2 h prior to his presentation to the hospital, and his left upper lateral incisor was completely knocked out of the alveolar socket. The diagnoses included avulsion of tooth 22, complicated crown fracture of tooth 11 and complicated crown-root fracture of tooth 21. The avulsed teeth were reimplanted along with autologous PRF granules and splinted using a semiflexible titanium preshaped labial arch. The root canals of the avulsed teeth were filled with calcium hydroxide paste, and root canal ﬁlling was performed 4 wk after reimplantation. The reimplanted teeth showed no symptoms of inﬂammatory root resorption or ankylosis at the 3-, 6-, and 12-mo follow-up examinations after reimplantation with autologous PRF. In addition to the avulsed teeth, the other injured teeth were treated using corresponding conventional treatment methods. CONCLUSION These cases provide examples of the successful use of PRF to reduce pathological root resorption of the avulsed teeth, and the application of PRF may provide new healing opportunities for traditionally “hopeless” avulsed teeth.

show abstract

Use of Platelet-rich fibrin as an autologous biologic rejuvenating media for avulsed teeth - anin vitrostudy

Abstract: Within the parameters of this study, a combination of platelet-rich fibrin and PPP demonstrated higher number of viable PDL cells and hence could be a good biologic rejuvenating media for avulsed teeth.

Cited by 6 publications

References 30 publications

Which is the most recommended medium for the storage and transport of avulsed teeth? A systematic review

Which is the most recommended medium for the storage and transport of avulsed teeth? A systematic review

Assessment of platelet-rich fibrin in the maintenance and recovery of cell viability of the periodontal ligament

Rescuing “hopeless” avulsed teeth using autologous platelet-rich fibrin following delayed reimplantation: Two case reports

Contact Info

Product

Resources

About