Use of plant genotoxicity bioassay for the evaluation of efficiency of algal biofilters in bioremediation of toxic industrial effluent

Migid, Hala M. Abdel; Azab, Yehia A.; Ibrahim, Waeel M.

doi:10.1016/j.ecoenv.2005.10.011

Cited by 61 publications

(15 citation statements)

References 21 publications

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“…En plus des effets directs sur la santé, le subtil danger des polluants toxiques et mutagènes est qu'ils provoquent des afflictions telles que le cancer, athérosclérose, les maladies cardiovasculaires et le vieillissement prématuré (Grover et Kaur, 1999). Plusieurs études menées sur les eaux usées provenant des décharges industrielles ou urbaines ont démontré l'existence des activités génotoxiques (Grover et Kaur, 1999 ;Lah et al, 2004 ;Abdel-Migid et al, 2007 ;Junior et al, 2007). L'intérêt sans cesse croissant qu'on porte à la génotoxicité causée par les polluants environnementaux est dû au fait qu'il entraine le développement de différents tests biologiques utilisés pour la détection et l'identification des génotoxiquants dans l'air, l'eau et dans le sol (Grisolia et Cordeiro, 2000).…”

Section: Résuméunclassified

Évaluation de la cytogénotoxicité des sites de pollution du PONT et d’ACCRON de la lagune de Porto- Novo

Cakpo¹,

Sagbo²,

Mama³

et al. 2015

J. App. Bioscience.

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RÉSUMÉObjectif : Les effets cytogénotoxiques des sites du PONT et d'ACCRON de la lagune de Porto-Novo à des concentrations de 0%, 25%, 50%, 75%, 100% ont été évalués par le test de toxicité basé sur les cellules des racines des oignons. Méthodologie et résultats : Dans cette étude, la longueur des racines et le test sur les aberrations chromosomiques ont été utilisés pour déterminer la concentration efficace (96h, CE50), l'inhibition de la croissance des racines, l'indice mitotique et le taux d'aberration chromosomique. D'après les résultats obtenus, les eaux du site du PONT sont 2,24 fois plus toxiques que celles des eaux du site d'ACCRON et on a une différente significative entre la moyenne des longueurs des racines des oignons exposés dans les différentes concentrations des deux sites. Cela indique que l'inhibition de la croissance des racines dépend des concentrations. L'indice mitotique (IM) croit avec l'augmentation des concentrations des eaux sur le site d'ACCRON par contre sur le site du PONT, il diminue de 0% à 25% et croît de 50% à 100%. Les eaux des deux sites ont provoqué des aberrations chromosomiques des cellules des racines des oignons avec des malformations chromosomiques telles que les chromosomes en fragments de pont, vagabonds et collants. Les aberrations communes observées lorsque les concentrations sont faibles, sont les chromosomes en fragments de pont, visqueux, vagabonds et les anaphases polaires. Le test de génotoxicité effectué sur les chromosomes des racines des oignons a permis de mesurer les effets génotoxiques des eaux des deux sites étudiés. Conclusion et application des résultats : Les tests de cytogénotoxicité basés sur les racines d'oignons effectués sur les eaux des deux sites ont montré d'une part que les deux sites sont génotoxiques et que le site du PONT est plus toxique que celui d'ACCRON d'autre part. Mots Clés : Cytogénotoxicité, oignons, pollution, aberrations chromosomiques, inhibition de la croissance des racines. Journal of Applied Biosciences 87:8076-8084 ISSN 1997-5902Cakpo et al. J. Appl. Biosci. Évaluation de la cytogénotoxicité des sites de pollution du PONT et d'ACCRON de la lagune de Porto-Novo 8077ABSTRACT Objective: The effects cytogenotoxicity at the sites of PONT and ACCRON of Porto-Novo lagoon were evaluated using root tip cells of Allium cepa. Methodology and results: In this study, root length and chromosomal aberration assays were used to determine the 96 h effective concentration (96h, EC 50 ) roots growth inhibition, mitotic index and chromosome aberration rate. According to the results obtained, water of the site of the PONT was 2.24 times more toxic than the water of ACCRON site and one has different significant between the average lengths of the roots of onions exposed in the various concentrations of the two sites. This indicated that the root growth inhibition was concentration dependent. The mitotic index (MI) increased with increasing concentrations of ACCRON site on the other hand on the PONT site; it decreased by 0% to 25% and grew from 50% t...

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Section: Résuméunclassified

Évaluation de la cytogénotoxicité des sites de pollution du PONT et d’ACCRON de la lagune de Porto- Novo

Cakpo¹,

Sagbo²,

Mama³

et al. 2015

J. App. Bioscience.

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“…Cytotoxicity and environmental pollution have been assessed by the in vivo onion (Allium cepa) root tip cell test which is known to give similar results to in vitro animal cytotoxicity tests (Vicentini et al, 2001;Teixeira et al, 2003). A. cepa test has also been used by many authors to evaluate cytotoxicity and genotoxicity of industrial effluents (Rank and Nielson, 1998;Chauhan et al, 1999;Grover and Kaur, 1999;El-Shahaby et al, 2003, Babatunde andBakare, 2006;Junior et al, 2007;Abdel-Migid et al, 2007;Şık et al, 2009;Samuel et al, 2010).…”

Section: Industriesmentioning

confidence: 99%

Genotoxicity Screening of Industrial Effluents using Onion bulbs (<i>Allium cepa L.</i>)

Olorunfemi¹,

Ogieseri²,

Akinboro³

2011

Journal of Applied Sciences and Environmental Management

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ABSTRACT:The potential cytotoxicity and genotoxicity of three industrial wastewaters (brewery, rubber and bottling) in Benin metropolis using the Allium cepa test were investigated. A series of five small onion bulbs were cultivated in 0.01, 0.1, 1, and 10% of the wastewaters (v/v) and after 48 hours, one root tip from each bulb was harvested and processed for cytological studies by the aceto-orcein squash technique. At 72h, their cytotoxic effects on the root tips showed strong growth retardation in high concentrations of all the wastewaters. Compared to the control, treatment with the wastewaters resulted in root growth inhibition with EC50 values of 35, 50 and 62% for bottling, rubber and brewery effluents respectively, and decrease in mitotic index with increasing concentration for all samples and these were statistically significant (p<0.05). Chromosomal aberrations induced in the onion root tip cells were mostly sticky chromosomes and bridges. Chromosomes with disturbed spindles and fragments were also present in appreciable amounts. Based on the EC50 values, the bottling wastewater was most toxic, followed by rubber effluent while effluents from the brewery were least toxic. The findings in this study indicate that there are toxic chemicals present in the wastewaters which are responsible for the observed genotoxic effects on the onion root tip cells. The study also reveals that the Allium test is a useful and reliable tool for the genotoxicity screening of industrial effluents which could be employed by environmental managers before these effluents are finally discharged into the environment. @JASEM

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“…В результате агглютинации расхождения хро-мосом не происходит и клетки обычно гибнут [28,34]. Агглютинация также наблюдалась в клетках лука под действием различных химических веществ: ионов меди и кобальта в высоких концентрациях [20,34], бензо(а)-пирена [17] и жидких стоков химзавода [14]. В иссле-дованных нами пробах элодеи из района Атаманово наряду с агглютинацией была отмечена повышенная частота клеток с фрагментацией хромосом (до 2 % кле-ток на стадии метафазы).…”

Section: Discussionunclassified

Types of cytogenetic abnormalities in apical root meristem of Elodea сanadensis from Yenisei River areas with different types of anthropogenic pollution

Medvedeva

Bolsunovsky

2016

Ecological genetics

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Background. The spectrum of cytogenetic abnormalities in the apical root meristem of Elodea сanadensis Michx. (elodea) from the Yenisei River was studied depending on the type of anthropogenic pollution. We compared elodea samples from areas with radiation-contaminated sediments (area affected by Rosatom Mining and Chemical Combine, MCC, Zheleznogorsk) and areas with chemical pollution (Krasnoyarsk). Materials and methods. The apical root meristem of elodea was fixed in acetic alcohol (96 % ethanol and glacial acetic acid, 3 : 1) and stained with 1 % aceto-orcein. The cytogenetic analysis of metaphase and ana-telophase elodea cells was carried out with temporal squashed preparations using an Olympus CX31 microscope. Results. At metaphase stage, the predominant types of abnormalities in elodea cells were disoriented chromosomes (up to 9 % of total metaphase cells) and chromosome agglutination (6 %). In the area affected by the MCC an increased content of ring chromosomes in elodea cells was detected, but in terms of frequency of their occurrence no significant differences were revealed between samples from areas with different types of pollution. Among abnormalities at ana-telophase stage, bridges (to 20% of dividing cells) and disoriented chromosomes (up to 8%) dominated. The following abnormalities were also detected: multipolar and asymmetrical mitoses, agglutination and cells with multiple chromosome abnormalities. It was shown that in areas with the highest content of 137Cs in bottom sediments the frequency of cells with bridges and multiple chromosome abnormalities significantly increased as compared to samples from other areas. Conclusion. In the radioactive contamination area of the Yenisei River the spectrum of abnormalities was dominated by chromosome aberrations (bridges, agglutination, chromosome fragmentation) and multiple abnormalities in one and the same cell. In areas with non-radioactive (chemical) contamination of sediments, mitotic spindle irregularities prevailed.

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Use of plant genotoxicity bioassay for the evaluation of efficiency of algal biofilters in bioremediation of toxic industrial effluent

Cited by 61 publications

References 21 publications

Évaluation de la cytogénotoxicité des sites de pollution du PONT et d’ACCRON de la lagune de Porto- Novo

Évaluation de la cytogénotoxicité des sites de pollution du PONT et d’ACCRON de la lagune de Porto- Novo

Genotoxicity Screening of Industrial Effluents using Onion bulbs (<i>Allium cepa L.</i>)

Types of cytogenetic abnormalities in apical root meristem of Elodea сanadensis from Yenisei River areas with different types of anthropogenic pollution

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