Use of plant cell cultures to study the metabolism of environmental chemicals

Sandermann, Heinrich; Scheel, Dierk; Trenck, Th.v.d.

doi:10.1016/0147-6513(84)90059-9

Cited by 72 publications

(48 citation statements)

References 24 publications

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“…Cell cultures of callus from potato roots (Solanum tuberosum L.) have been used as a model to evaluate the phytotoxicity and the metabolism of several herbicides (Sandermann et al, 1984). The absence of a complex structural organization and a more efficient exposition to environmental stress are advantages of this sensitive model for toxicity evaluation (Smeda and Weller, 1991).…”

Section: Introductionmentioning

confidence: 99%

Comparative effects of the herbicides dicamba, 2,4-D and paraquat on non-green potato tuber calli

Peixoto

Gomes-Laranjo

Vicente

et al. 2008

Journal of Plant Physiology

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SummaryThe effects of the herbicides 1,1 0 -dimethyl-4,4 0 -bipyridylium dichloride (paraquat), 3,6-dichloro-2-metoxybenzoic acid (dicamba) and 2,4-dichlorophenoxyacetic acid (2,4-D) on cell growth of non-green potato tuber calli are described. We attempted to relate the effects with toxicity, in particular the enzymes committed to the cellular antioxidant system. Cell cultures were exposed to the herbicides for a period of 4 weeks. Cellular integrity on the basis of fluorescein release was strongly affected by 2,4-D, followed by dicamba, and was not affected by paraquat. However, the three herbicides decreased the energy charge, with paraquat and 2,4-D being very efficient. Paraquat induced catalase (CAT) activity at low concentrations (1 mM), whereas at higher concentrations, inhibition was observed. Dicamba and 2,4-D stimulated CAT as a function of concentration. Superoxide dismutase (SOD) activity was strongly stimulated by paraquat, whereas dicamba and 2,4-D were efficient only at higher concentrations. Glutathione reductase (GR) activity was induced by all the herbicides, suggesting that glutathione and glutathione-dependent enzymes are putatively involved in the detoxification of these herbicides. Paraquat slightly inhibited glutathione S-transferase (GST), whereas 2,4-D and dicamba promoted significant activation. These results indicate that the detoxifying mechanisms for 2,4-D and dicamba may be different from the mechanisms of paraquat detoxification. However, the main cause of cell death induced by paraquat and 2,4-D is putatively related with the cell energy charge decrease.

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Section: Introductionmentioning

confidence: 99%

Comparative effects of the herbicides dicamba, 2,4-D and paraquat on non-green potato tuber calli

Peixoto

Gomes-Laranjo

Vicente

et al. 2008

Journal of Plant Physiology

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“…There is a general expectation that uptake of contaminants will be greater in plant cell cultures than in whole plants, reflecting the uniform exposure of cultured cell surfaces to the external environment and the fact that translocation is not required (Camper and McDonald, 1989;Sandermann et al, 1984). Yet the literature does not provide consistent support for this expectation, as enhanced (Harms, 1992), reduced Nedelkoska and Doran, 2001) and comparable (Van Aken and Schnoor, 2002) rates and levels of uptake of organic and inorganic pollutants have all been reported for plant cultures relative to whole plants.…”

Section: Uptake Studiesmentioning

confidence: 99%

“…The ability to manipulate in vitro cultures to achieve certain desirable metabolic or tolerance outcomes highlights the need for standardization of the experimental methods applied to plant cell cultures Sandermann et al, 1984;Schmidt, 2001).…”

Section: The Outcome Of Plant Tissue Culture Studies Depends On the Cmentioning

confidence: 99%

Application of plant tissue cultures in phytoremediation research: Incentives and limitations

Doran

2009

Biotech & Bioengineering

151

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The aim of this review is to critically assess the benefits and limitations associated with the use of in vitro plant cell and organ cultures as research tools in phytoremediation studies. Plant tissue cultures such as callus, cell suspensions, and hairy roots are applied frequently in phytoremediation research as model plant systems. In vitro cultures offer a range of experimental advantages in studies aimed at examining the intrinsic metabolic capabilities of plant cells and their capacity for toxicity tolerance. The ability to identify the contributions of plant cells to pollutant uptake and detoxification without interference from microorganisms is of particular significance in the search for fundamental knowledge about plants. However, if the ultimate goal of plant tissue culture experiments is the development of practical phytoremediation technology, the limitations inherent in the use of in vitro cultures as a representative of whole plants in the field must be recognized. The bioavailability of contaminants and the processes of pollutant uptake and metabolite distribution are likely to be substantially different in the two systems; this can lead to qualitative as well as quantitative differences in metabolic profiles and tolerance characteristics. Yet, many studies have demonstrated that plant tissue cultures are an extremely valuable tool in phytoremediation research. The results derived from tissue cultures can be used to predict the responses of plants to environmental contaminants, and to improve the design and thus reduce the cost of subsequent conventional whole plant experiments.

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“…Additionally, in callus tissue cultures, the simplicity of the structural organization and the absence of cellular wall barrier amenable to an effective herbicide penetration and translocation (Mumma & Davidonis, 1983;Magalhães et al, 1989;Nellessen & Fletcher, 1993) make this sensitive model a useful tool to study herbicide toxicity and metabolism in plants (Sandermann et al, 1984;Smeda & Weller, 1991). The growth of nongreen callus tissue in the dark precludes the analysis of herbicide effects on the photosynthetic system, but allows to address studies to other putative targets of the toxic action of those compounds, such as mitochondria which have a crucial role in ATP synthesis in these cells devoid of chlorophyl.…”

Section: Cell Cultures Of Plant Tissues An Economical Model To mentioning

confidence: 99%

Herbicides: the Face and the Reverse of the Coin. An in Vitro Approach to the Toxicity of Herbicides in Non-Target Organisms

Jurado¹,

Férnandes²,

Videira³

et al. 2011

Herbicides and Environment

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Use of plant cell cultures to study the metabolism of environmental chemicals

Cited by 72 publications

References 24 publications

Comparative effects of the herbicides dicamba, 2,4-D and paraquat on non-green potato tuber calli

Comparative effects of the herbicides dicamba, 2,4-D and paraquat on non-green potato tuber calli

Application of plant tissue cultures in phytoremediation research: Incentives and limitations

Herbicides: the Face and the Reverse of the Coin. An in Vitro Approach to the Toxicity of Herbicides in Non-Target Organisms

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