Use of plankton-derived vitamin B1 precursors, especially thiazole-related precursor, by key marine picoeukaryotic phytoplankton

Paerl, Ryan W.; Bouget, François‐Yves; Lozano, Jean-Claude; Vergé, Valérie; Schatt, Philippe; Allen, Eric E.; Palenik, Brian; Azam, Farooq

doi:10.1038/ismej.2016.145

Cited by 51 publications

(83 citation statements)

References 42 publications

(55 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In another study, marine bacteria were shown to produce a thiazole precursor used by Ostreococcus species to synthesize thiamine. 36 These findings are analogous to our past findings in co-cultures of E. coli with A. protothecoides and indicate the widespread importance of cofactor symbiosis in promoting algae growth.…”

Section: Discussionsupporting

confidence: 78%

Algal–bacterial synergy in treatment of winery wastewater

et al. 2018

View full text Add to dashboard Cite

There is significant potential for employing algae in tertiary wastewater treatment, however, little is known about the contribution of algae-bacteria synergy toward treatment performance. This study demonstrates potential synergy in the treatment of three winery wastewater samples. Two strains of green algae, Auxenochlorella protothecoides and Chlorella sorokiniana were tested and each removed > 90% of nitrogen, > 50% of phosphate, and 100% of acetic acid in the wastewater. Both algae strains grew significantly faster on wastewaters compared to growth on minimal media. Organic carbon in the wastewater apparently played a limited role in algal growth enhancement. When cultured on sterile-filtered wastewater, A. protothecoides increased soluble COD loadings in two of the three wastewaters and C. sorokiniana secreted an insoluble film. Culturing algae with the native wastewater microbial community negated the secretion of algal photosynthate, allowing for simultaneous reductions in COD and nutrient concentrations. Both algae species stimulated bacterial growth in a strain-specific way, suggesting unique responses to algal photosynthate. Cofactor auxotrophy for thiamine, cobalamin, and biotin is widespread among algae and these cofactors are typically obtained from bacteria. Sequencing the wastewater microbial community revealed bacteria capable of synthesizing all three cofactors while liquid chromatography with mass spectrometry (LCMS) and bio-assays revealed the presence of thiamine metabolites in the wastewaters. These cofactors likely increased algal growth rates, particularly for A. protothecoides, which cannot synthesize thiamine de-novo but can salvage it from degradation products. Collectively, these results demonstrate that bacteria and algae provided synergistic growth benefits, potentially contributing to higher levels of wastewater treatment than either organism type alone.

show abstract

Section: Discussionsupporting

confidence: 78%

Algal–bacterial synergy in treatment of winery wastewater

et al. 2018

View full text Add to dashboard Cite

show abstract

“…S7, Table S7). Our observations suggest that Euryarchaeota and Thaumarchaeota may be a source of vitamins B1 (thiamin), B2, B6, and B9 (folate) in the water column, analogous to observations on the provision of vitamins such as thiamin (B1) (Paerl et al 2017) and cobalamin (B12) (Bonnet et al 2010) by certain members of the community. This is further supported by the discovery of a complete cobalamin biosynthesis pathway in Thaumarchaeota (Doxey et al 2015).…”

Section: Archaeamentioning

confidence: 60%

Winter mixing impacts gene expression in marine microbial populations in the Gulf of Aqaba

Miller¹,

Pfreundt²,

Hou³

et al. 2017

Aquat. Microb. Ecol.

View full text Add to dashboard Cite

In aquatic systems, changes in temperature and irradiance fundamentally characterize the water column and regulate microbial population structure and function. In systems with stable thermal stratification, the warm surface mixed layer is typically nutrient impoverished, limiting biological production. In periods of destratification, convective mixing of the water column exposes the microorganisms inhabiting these mixed systems to rapid variations in light availability and spectra. We explored the impact of winter deep-mixing (500 m deep mixed layer) on microbial communities from the surface (2.5 m) and the aphotic waters (440 m) in the Gulf of Aqaba by examining changes in both population composition and function via DNA and RNA sequencing. The greatest fraction of 16S sequences was assigned to Euryarchaeota, while metatranscriptomes were dominated by Synechococcus transcripts. Community composition was highly similar at both depths, yet transcription profiles differed. Phototrophic organisms found at the photic surface overexpressed genes related to catabolism and energy metabolism, while genes affiliated with biosynthesis were overexpressed at the aphotic depth. Similar transcriptional trends were observed in the non-photoautotrophs SAR11, Euryarchaeota, and Thaumarchaeota, with niche partitioning based on differential utilization of nitrogen and phosphorus occurring between the 2 archaeal groups. We did not detect upregulated expression of cyanobacterial genes indicative of mixotrophy or glycogen metabolism in the aphotic zone, suggesting they survive the aphotic period by utilizing photosynthates produced in the photic zone. Indications for a mixotrophic lifestyle were observed for prasinophytes, with genes related to phagocytosis overexpressed at the aphotic depth compared with the surface.

show abstract

“…In return, heterotrophic bacteria receive nutrients and can consume up to 82% of all algal-derived organic matter (Hornak et al, 2017). The release of vitamins and vitamin precursors by bacteria is one of the most common and well studied means mediating algaeÀbacteria interactions (Croft et al, 2005;Paerl et al, 2017). Vitamin transfer supports algal growth and can be considered as bacterial farming of algae as suppliers of organic resources.…”

Section: Cross-kingdom Exchange Of Resourcesmentioning

confidence: 99%

Algae−bacteria interactions that balance the planktonic microbiome

2019

View full text Add to dashboard Cite

Summary Phytoplankton communities within the photic zones of the oceans and lakes are characterised by highly complex assemblages of unicellular microalgae and associated bacteria. The interconnected evolutionary history of algae and bacteria allowed the formation of a wide spectrum of associations defined by orchestrated nutrient exchange, mutual support with growth factors, quorum sensing mediation, and episodic killing of the partners to obtain more resources. In this review, we discuss how these cross‐kingdom interactions shape plankton communities that undergo annual, seasonal switching between alternative states with balanced multispecies consortia. We illustrate how these microscopic interactions can have consequences that scale up to influence global element cycling.

show abstract

Use of plankton-derived vitamin B1 precursors, especially thiazole-related precursor, by key marine picoeukaryotic phytoplankton

Cited by 51 publications

References 42 publications

Algal–bacterial synergy in treatment of winery wastewater

Algal–bacterial synergy in treatment of winery wastewater

Winter mixing impacts gene expression in marine microbial populations in the Gulf of Aqaba

Algae−bacteria interactions that balance the planktonic microbiome

Contact Info

Product

Resources

About