Use of Photodynamic Therapy and Sterile Water to Target Adipose Tissue

Wanner, Molly; Mihm, Martín C.; Farinelli, William A.; Doukas, Apostolos G.; Zurakowski, David; Piris, Adriano; Avram, Mathew M.; Klein, Jeffrey A.; Anderson, R. Rox

doi:10.1097/dss.0000000000000385

Cited by 7 publications

(6 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When the body weight of the mice increased by 70% with HFD (HFD containing 60% fat) in 10 weeks, we randomly assigned them to four groups: vehicle-treated HFD only; high-fat diet with Ce6, 2.5 mg/kg, and light of a high fluence rate (HC2.5Hi); high-fat diet with Ce6, 5 mg/kg, and light of a low fluence rate (HC5Lo); and high-fat diet with Ce6, 5 mg/kg, and light of a high fluence rate (HC5Hi) ( Table 3 ). The mice were then administered an oral dose of Ce6 (2.5 or 5 mg/kg), and 3 h [ 29 ] later, PDT was performed using a 660 nm laser irradiation (2.56 mW/cm 2 and 4.96 mW/cm 2 ), while vehicle-treated HFD mice were administered with normal saline. PDT was repeated every 2 days for up to 4 weeks.…”

Section: Resultsmentioning

confidence: 99%

“…Obesity is considered an epidemic that is rapidly spreading across the globe and causing a variety of metabolic diseases [ 1 ]. In recent years, PDT has become an alternative choice for individuals to reduce their weight amidst the hustle of life, besides various therapeutic treatments for obesity and its related metabolic disorders [ 26 , 27 , 28 , 29 , 30 ]. For the first time, the two different murine models of obesity investigated the pharmacokinetic properties and ADME studies as well as evaluated the anti-obesity effects of Ce6-PDT by using PDT chamber systems.…”

Section: Discussionmentioning

confidence: 99%

“…In particular, liposomal formulations of photosensitizers or the systemic administration of drugs followed by photoactivation with a given wavelength and light energy are known to selectively target subcutaneous adipose tissue [ 25 ]. In recent years, PDT with methylene blue, veteporfin, and indocyanine green has been known to reduce fat [ 27 , 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Anti-Obesity Effect of Chlorin e6-Mediated Photodynamic Therapy on Mice with High-Fat-Diet-Induced Obesity

et al. 2023

View full text Add to dashboard Cite

This study aimed to evaluate the efficacy of Chlorin e6 (Ce6)-based photodynamic therapy (PDT) for anti-obesity activities in high-fat-diet (HFD)-induced obesity mouse models. We induced obesity in C57BL/6 mice by HFD and administered Ce6 (2.5 or 5 mg/kg) orally with 3 h of incubation. The mice were then exposed to light of high fluence rate (4.96 mW/cm2) or low fluence rate (2.56 mW/cm2) in the designed LED mouse chamber 2–3 days a week for up to 8 weeks. The study also analyzed the pharmacokinetics and optimization of the drug by evaluating the absorption, distribution, metabolism, and excretion (ADME) of Ce6 in the rat models. Both low doses (2.5 mg/kg) and high doses (5 mg/kg) of Ce6 with high irradiation dose showed better anti-obesity effects than other groups with decreased body weight. The lipid accumulation in the liver and adipocyte size in epididymal adipose tissues were found to be decreased by Ce6-PDT in comparison to vehicle-treated HFD groups. We also observed increased levels of the lipidomic biomarkers, such as leptin and LDL cholesterol, while observing decreasing levels of total cholesterol and adiponectin in the Ce6-PDT-treated mice. These findings may provide valuable insight into Ce6-PDT as an alternative and non-invasive therapeutic methodology for obesity and obesity-related diseases.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Anti-Obesity Effect of Chlorin e6-Mediated Photodynamic Therapy on Mice with High-Fat-Diet-Induced Obesity

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Photodynamic therapy (PDT) is a therapeutic method used to treat tumors as well as some skin diseases, such as psoriasis and acne, by means of photosensitizers that enter the body and are activated by light of appropriate wavelengths, resulting in a series of photochemical toxic effects, i.e., the generation of singlet oxygen ( 1 O 2 ) and other reactive oxygen species (ROS) that bind to the corresponding target tissues, leading to tissue damage and cell death. Local PDT mediated by verteporfin or indocyanine green was previously reported to reduce fat [ 129 ]. PDT acts directly and rapidly but is only applicable to superficial fat due to the limitation of light penetration.…”

Section: Biomaterial-assisted Anti-obesity Therapymentioning

confidence: 99%

Biomaterial-Based Therapeutic Strategies for Obesity and Its Comorbidities

Duan

Liu

et al. 2022

Pharmaceutics

View full text Add to dashboard Cite

Obesity is a global public health issue that results in many health complications or comorbidities, including type 2 diabetes mellitus, cardiovascular disease, and fatty liver. Pharmacotherapy alone or combined with either lifestyle alteration or surgery represents the main modality to combat obesity and its complications. However, most anti-obesity drugs are limited by their bioavailability, target specificity, and potential toxic effects. Only a handful of drugs, including orlistat, liraglutide, and semaglutide, are currently approved for clinical obesity treatment. Thus, there is an urgent need for alternative treatment strategies. Based on the new revelation of the pathogenesis of obesity and the efforts toward the multi-disciplinary integration of materials, chemistry, biotechnology, and pharmacy, some emerging obesity treatment strategies are gradually entering the field of preclinical and clinical research. Herein, by analyzing the current situation and challenges of various new obesity treatment strategies such as small-molecule drugs, natural drugs, and biotechnology drugs, the advanced functions and prospects of biomaterials in obesity-targeted delivery, as well as their biological activities and applications in obesity treatment, are systematically summarized. Finally, based on the systematic analysis of biomaterial-based obesity therapeutic strategies, the future prospects and challenges in this field are proposed.

show abstract

“…It has been reported that PDT mediated by photosensitizers (PSs) can reduce fats since reactive oxygen species (ROS) produced by PSs could rapidly drive adipocyte death upon laser illumination. [11][12][13][14][15] In the meantime, the other strategy for obesity treatment is adipose browning induction, which could transform white adipose tissue (WAT) into thermogenic adipose tissue. [16,17] WAT exists in excess energy in the form of triacylglycerol (TAG), while thermogenic adipose tissue dissipates energy as heat.…”

Section: Introductionmentioning

confidence: 99%

Adipose Tissue Targeting Ultra‐Small Hybrid Nanoparticles for Synergistic Photodynamic Therapy and Browning Induction in Obesity Treatment

Ma,

Jian,

Guo

et al. 2023

Small

View full text Add to dashboard Cite

Photodynamic therapy (PDT), as a means of locally and rapidly inducing adipocyte death via light illumination, in combination with adipose browning induction, a more gradual and widespread effect that could transform white adipose tissue into thermogenic adipose tissue, manifests a promising approach to combat obesity. Herein, adipose‐targeting ultra‐small hybrid nanoparticles (Pep‐PPIX‐Baic NPs) composed of an adipose‐targeting peptide, Fe3+, a photosensitizer (protoporphyrin IX), and a browning agent (baicalin) are introduced. Pep‐PPIX‐Baic NPs have been designed to simultaneously enhance the photodynamic effect and induce browning. After intravenous injection in obese mice, the hybrid nanoparticles can specifically accumulate in white adipose tissues, especially those rich in blood supply, and drive adipose reduction owing to the synergy of the PDT effect and baicalin browning induction. Overall, Pep‐PPIX‐Baic NPs exhibited superior anti‐obesity potential through PDT synergistic with adipose browning induction. The designed multifunctional adipose‐targeting hybrid nanoparticles present a prospective nanoplatform for obesity treatment.

show abstract

Use of Photodynamic Therapy and Sterile Water to Target Adipose Tissue

Abstract: Verteporfin decreased adipose tissue after treatment. Sterile water injection had a statistically significant effect on adipose tissue histologically but did not substantially decrease the adipose tissue by ultrasound 4 to 6 weeks after intervention. Longer follow-up may be needed.

Cited by 7 publications

References 16 publications

Anti-Obesity Effect of Chlorin e6-Mediated Photodynamic Therapy on Mice with High-Fat-Diet-Induced Obesity

Anti-Obesity Effect of Chlorin e6-Mediated Photodynamic Therapy on Mice with High-Fat-Diet-Induced Obesity

Biomaterial-Based Therapeutic Strategies for Obesity and Its Comorbidities

Adipose Tissue Targeting Ultra‐Small Hybrid Nanoparticles for Synergistic Photodynamic Therapy and Browning Induction in Obesity Treatment

Contact Info

Product

Resources

About