Use of phenolic compounds for sensitizing Listeria monocytogenes to high-pressure processing

Vurma, Mustafa; Chung, Yoon-Kyung; Shellhammer, Thomas H.; Turek, Evan J.; Yousef, Ahmed E.

doi:10.1016/j.ijfoodmicro.2005.06.025

Cited by 21 publications

(23 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Several workers have reported the lethal effect of HHP in combination with phenolic antioxidants, such as TBHQ or butylated hydroxyanisole (8,27,58), or with plant essential oils, such as citral (41).…”

Section: Discussionmentioning

confidence: 99%

“…TBHQ and HHP treatment also act synergistically when simultaneously applied to L. monocytogenes at pH 7.0 (8,58). We found that 100 ppm of TBHQ acting alone at pH 7.0 did not affect the survival of L. monocytogenes, in agreement with Vurma et al (58), but caused extensive sublethal injury following refrigerated storage for 24 h. This sensitizing effect might explain the efficacy of the combined treatment of HHP and TBHQ in L. monocytogenes, which allowed the inactivation of Ͼ6 log 10 cycles at pH 7.0. Conversely, E. coli cells were not sensitized by the presence of TBHQ alone, but a synergistic killing effect of HHP and TBHQ was observed after incubation for 24 h at refrigeration temperatures, also allowing the inactivation of Ͼ6 log 10 cycles of E. coli cells.…”

Section: Discussionmentioning

confidence: 99%

“…To the best of our knowledge, the combined effect of HHP and TBHQ on a gram-negative bacterial cell has not previously been investigated. Several authors have suggested that phenolic compounds might affect the cell envelopes (50,57,58). The synergistic effect might result from the same component of the microbial cell, the cytoplasmic membrane, being targeted simultaneously by both HHP and TBHQ agents.…”

Section: Discussionmentioning

confidence: 99%

“…Published data have demonstrated that the application of specific additional hurdles such as antimicrobial peptides, the lactoperoxidase system, and phenolic compounds in combination with HHP treatments have synergistic inactivation effects (8,16,17,18,21,22,30,31,34,55,58). One of the most effective phenolic compounds is tertbutyl hydroquinone (TBHQ), which sensitizes some barotolerant Listeria monocytogenes strains to HHP (8,58).…”

mentioning

confidence: 99%

“…Where indicated, 100 ppm of TBHQ or 1,000 ppm of citral was added to the buffer. These concentrations were chosen from those previously described in published literature (8,11,58). Cell suspensions (2 ml each) were then placed in sterile plastic pouches that were heat sealed and kept on ice before pressurization.…”

mentioning

confidence: 99%

See 4 more Smart Citations

Relationship between Sublethal Injury and Microbial Inactivation by the Combination of High Hydrostatic Pressure and Citral or tert -Butyl Hydroquinone

Somolinos¹,

García²,

Pagán³

et al. 2008

Appl Environ Microbiol

View full text Add to dashboard Cite

The aim was to investigate (i) the occurrence of sublethal injury in Listeria monocytogenes, Escherichia coli, and Saccharomyces cerevisiae after high hydrostatic pressure (HHP) treatment as a function of the treatment medium pH and composition and (ii) the relationship between the occurrence of sublethal injury and the inactivating effect of a combination of HHP and two antimicrobial compounds, tert-butyl hydroquinone (TBHQ) and citral. The three microorganisms showed a high proportion of sublethally injured cells (up to 99.99% of the surviving population) after HHP. In E. coli and L. monocytogenes, the extent of inactivation and sublethal injury depended on the pH and the composition of the treatment medium, whereas in S. cerevisiae, inactivation and sublethal injury were independent of medium pH or composition under the conditions tested. TBHQ alone was not lethal to E. coli or L. monocytogenes but acted synergistically with HHP and 24-h refrigeration, resulting in a viability decrease of >5 log 10 cycles of both organisms. The antimicrobial effect of citral depended on the microorganism and the treatment medium pH. Acting alone for 24 h under refrigeration, 1,000 ppm of citral caused a reduction of 5 log 10 cycles of E. coli at pH 7.0 and almost 3 log 10 cycles of L. monocytogenes at pH 4.0. The combination of citral and HHP also showed a synergistic effect. Our results have confirmed that the detection of sublethal injury after HHP may contribute to the identification of those treatment conditions under which HHP may act synergistically with other preserving processes.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Relationship between Sublethal Injury and Microbial Inactivation by the Combination of High Hydrostatic Pressure and Citral or tert -Butyl Hydroquinone

Somolinos¹,

García²,

Pagán³

et al. 2008

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

Sensitization of Microorganisms to High Pressure Processing by Phenolic Compounds

Chung¹,

Malone²,

Yousef³

et al. 2007

High Pressure Processing of Foods

View full text Add to dashboard Cite

Inactivation of barotolerant strains of Listeria monocytogenes and Escherichia coli O157:H7 by ultra high pressure and tert-butylhydroquinone combination

Chung

Yousef

2008

J Microbiol.

Self Cite

View full text Add to dashboard Cite

Antimicrobial efficacy of ultra-high-pressure (UHP) can be enhanced by application of additional hurdles. The objective of this study was to systematically assess the enhancement in pressure lethality by TBHQ treatment, against barotolerant strains of Escherichia coli O157:H7 and Listeria monocytogenes. Two L. monocytogenes Scott A and the barotolerant OSY-328 strain, and two E. coli O157:H7 strains, EDL-933 and its barotolerant mutant, OSY-ASM, were tested. Cell suspensions containing TBHQ (50 ppm, dissolved in dimethyl sulfoxide) were pressurized at 200 to 500 MPa (23+/-2 degrees C) for 1 min, plated on tryptose agar and enumerated the survivors. The TBHQ-UHP combination resulted in synergistic inactivation of both pathogens, with different degrees of lethality among strains. The pressure lethality threshold, for the combination treatment, was lower for E. coli O157:H7 (> or = 200 MPa) than for L. monocytogenes (> 300 MPa). E. coli O157:H7 strains were extremely sensitive to the TBHQ-UHP treatment, compared to Listeria strains. Interestingly, a control treatment involving DMSO-UHP combination consistently resulted in higher inactivation than that achieved by UHP alone, against all strains tested. However, sensitization of the pathogens to UHP by the additives (TBHQ in DMSO) was prominently greater for UHP than DMSO. Differences in sensitivities to the treatment between these two pathogens may be attributed to discrepancies in cellular structure or physiological functions.

show abstract

Use of phenolic compounds for sensitizing Listeria monocytogenes to high-pressure processing

Cited by 21 publications

References 22 publications

Relationship between Sublethal Injury and Microbial Inactivation by the Combination of High Hydrostatic Pressure and Citral or tert -Butyl Hydroquinone

Relationship between Sublethal Injury and Microbial Inactivation by the Combination of High Hydrostatic Pressure and Citral or tert -Butyl Hydroquinone

Sensitization of Microorganisms to High Pressure Processing by Phenolic Compounds

Inactivation of barotolerant strains of Listeria monocytogenes and Escherichia coli O157:H7 by ultra high pressure and tert-butylhydroquinone combination

Contact Info

Product

Resources

About