Use of PCR-DGGE based molecular methods to assessment of microbial diversity during anaerobic treatment of antibiotic combinations

Aydın, Sevcan; Shahi, Aiyoub; Özbayram, E. Gözde; İnce, Bahar; İnce, Orhan

doi:10.1016/j.biortech.2015.05.086

Cited by 54 publications

(21 citation statements)

References 32 publications

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“…Significant difference were determined at the p < 0.05 level of significance. Detailed information on the DGGE analysis has been reported in the previous study (Aydin et al 2015c). …”

Section: Discussionmentioning

confidence: 99%

“…Determination of microbial community dynamics in the anaerobic digesters using FISH analysis FISH analysis was used to determine the number of active cells of bacteria, archaea, and different phylogenetic groups of methanogens in control and OTC-containing anaerobic digesters (Aydin et al 2015c). Samples were taken from anaerobic digesters under varying operational conditions for FISH analysis on digestion days 0, 10, 20, and 30 in order to monitor the total number of active cells of different groups of microorganisms during the operation.…”

Section: Performance Of the Anaerobic Digestersmentioning

confidence: 99%

“…These primers have also been successfully used for PCR and PCR-DGGE by some other researchers (Kaiya et al 2012;Takahashi et al 2014). Arch344F-GC/Univ522R primers are suitable primers for amplification of the archaeal V3 region (Aydin et al 2015c). GC clamped form of these primers was used for amplification of bacterial and archaeal 16 rDNA genes.…”

Section: Dgge Analysismentioning

confidence: 99%

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Changes in microbial community structures due to varying operational conditions in the anaerobic digestion of oxytetracycline-medicated cow manure

Türker

Aydın

Akyol

et al. 2016

Appl Microbiol Biotechnol

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Management of manure containing veterinary antibiotics is a major concern in anaerobic treatment systems because of their possible adverse effects on microbial communities. Therefore, the aim of study was to investigate how oxytetracycline (OTC) influences bacteria and acetoclastic and hydrogenotrophic methanogens under varying operational conditions in OTC-medicated and non-medicated anaerobic cow manure digesters. Concentrations of OTC and its metabolites throughout the anaerobic digestion were determined using ultraviolet-high-performance liquid chromatography (UV-HPLC) and tandem liquid chromatography-mass spectrometry (LC/MS/MS), respectively. Fluorescent in situ hybridization, denaturing gradient gel electrophoresis, cloning, and sequencing analyses were used to monitor changes in microbial community structures. According to the results of analytical and molecular approaches, operating conditions highly influence active microbial community dynamics and associate with biogas production and elimination of OTC and its metabolites during anaerobic digestion of cow manure in the presence of an average initial concentration of 2.2 mg OTC/L. The impact of operating conditions has a drastic effect on acetoclastic methanogens than hydrogenotrophic methanogens and bacteria.

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“…Significant difference were determined at the p < 0.05 level of significance. Detailed information on the DGGE analysis has been reported in the previous study (Aydin et al 2015c). …”

Section: Discussionmentioning

confidence: 99%

Section: Performance Of the Anaerobic Digestersmentioning

confidence: 99%

Section: Dgge Analysismentioning

confidence: 99%

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Changes in microbial community structures due to varying operational conditions in the anaerobic digestion of oxytetracycline-medicated cow manure

Türker

Aydın

Akyol

et al. 2016

Appl Microbiol Biotechnol

Self Cite

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“…et al, 2008;Wei et al, 2012). These techniques have been used to characterize the dynamics of microbial evolution in various wastewater treatment reactors Zhang et al, 2010;Aydin et al, 2015). The molecular techniques are also applied in the MBR system to study the bacterial community structures.…”

Section: Introductionmentioning

confidence: 99%

Membrane fouling behavior and microbial community succession in a submerged membrane bioreactor treating harbor oily wastewater

Wei

2016

J. Zhejiang Univ. Sci. A

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Abstract:A membrane bioreactor (MBR) was established for treating harbor oily wastewater. It showed good removal performance for chemical oxygen demand (COD), oil content, suspended solids (SS), and other pollutants. However, serious membrane fouling occurred. It was recognized that the extracellular polymeric substances (EPS) accumulated on the membrane surface, especially the proteins, were of great importance for the transmembrane pressure (TMP) increment and membrane fouling. The MBR was optimized via improving aeration rate and reducing the ratio of A r /A d (A r and A d are the cross-sectional areas of the riser and the downcomer of the MBR). The increasing rate of TMP was slowed, indicating that the optimization strategy could effectively mitigate membrane fouling. Microbial community evolution was monitored and analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), cloning, and sequencing of 16S ribosomal ribonucleic acid (rRNA) fragments. Results revealed that low community shift occurred during the whole operational period. Geobacter sp. and Rhodocyclales sp., which have also been identified by other studies in a petroleum refinery wastewater MBR or an infiltration basin receiving highway runoff, dominated in the MBR system throughout. Comamonas sp. was thought to accommodate the lower aeration rate in this study, while Rhodocyclales sp. preferred the higher aeration rate. In addition, during the operational time under high organic loading rate, a few species were present in abundance, and may have been responsible for the good removal performance at this time.

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“…A different study of the same anaerobic digesters used quantitative PCR to quantify formate-tetrahydrofolate ligase (FTHFS), acetyl-coenzyme A synthetase (ACAS), and methyl coenzyme M reductase (mcrA) genes and reveal that this antibiotic mixture inhibited reactor performance by disrupting acetogenesis, acetoclastic methanogenesis, and hydrogenotrophic methanogenesis pathways, respectively [69]. Likewise, the use of DGGE analysis showed that acetoclastic methanogens were most directly impacted by increasing antibiotic concentrations [70]. A recent study by Xiong et al has further confirmed the previously discussed results by determining through high-throughput sequencing that both syntrophic VFA utilizing bacteria and methanogenic populations changed significantly at high concentrations of tetracycline in anaerobic reactors [60].…”

Section: Impact Of Antibiotics On the Anaerobic Digestion Processmentioning

confidence: 99%

Anaerobic Membrane Bioreactor Effluent Reuse: A Review of Microbial Safety Concerns

Harb¹,

Hong²

2017

Fermentation

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Broad and increasing interest in sustainable wastewater treatment has led a paradigm shift towards more efficient means of treatment system operation. A key aspect of improving overall sustainability is the potential for direct wastewater effluent reuse. Anaerobic membrane bioreactors (AnMBRs) have been identified as an attractive option for producing high quality and nutrient-rich effluents during the treatment of municipal wastewaters. The introduction of direct effluent reuse does, however, raise several safety concerns related to its application. Among those concerns are the microbial threats associated with pathogenic bacteria as well as the emerging issues associated with antibiotic-resistant bacteria and the potential for proliferation of antibiotic resistance genes. Although there is substantial research evaluating these topics from the perspectives of anaerobic digestion and membrane bioreactors separately, little is known regarding how AnMBR systems can contribute to pathogen and antibiotic resistance removal and propagation in wastewater effluents. The aim of this review is to provide a current assessment of existing literature on anaerobic and membrane-based treatment systems as they relate to these microbial safety issues and utilize this assessment to identify areas of potential future research to evaluate the suitability of AnMBRs for direct effluent reuse.

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Use of PCR-DGGE based molecular methods to assessment of microbial diversity during anaerobic treatment of antibiotic combinations

Cited by 54 publications

References 32 publications

Changes in microbial community structures due to varying operational conditions in the anaerobic digestion of oxytetracycline-medicated cow manure

Changes in microbial community structures due to varying operational conditions in the anaerobic digestion of oxytetracycline-medicated cow manure

Membrane fouling behavior and microbial community succession in a submerged membrane bioreactor treating harbor oily wastewater

Anaerobic Membrane Bioreactor Effluent Reuse: A Review of Microbial Safety Concerns

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