Use of organic waste for the production of added-value holocellulases with Cellulomonas flavigena PR-22 and Trichoderma reesei MCG 80

Abstract

We evaluated the production of holocellulases from the cellulolytic microorganisms Cellulomonas flavigena PR-22 and Trichoderma reesei MCG 80 using as substrates the organic fraction of municipal solid waste (OFMSW) and digestates from a hydrogenogenic-methanogenic bioenergy production process. The first set of experiments (E1) used the mutant actinobacteria C. flavigena PR-22 whereas another set (E2) used the mutant filamentous fungi T. reesei MCG 80. In E1 with OFMSW as substrate, xylanolytic activities ranged from 1800 to 3900 international units g(holocellulose)(-1) (IU g(hol)(-1)), whereas the cellulolytic activities ranged from 220 to 420 IU g(hol)(-1). The variation of agitation speed did not have a significant effect on enzyme activity, whereas the increase of substrate concentration had a significant negative effect on both xylanolytic and cellulolytic activities on a holocellulose feed basis. Regarding E2, the OFMSW was evaluated at 1, 2 and 3 % volatile solids (VS). At 2 % VS the best filter paper activities were 1200 filter paper units (FPU) l(-1); however, in a holocellulase basis the best result was 67 FPU g(hol)(-1), corresponding to 1 % VS. Next, OFMSW was compared with OFMSW supplemented with lactose, digested solids from hydrogenogenic fermentation (D1) and digested solids from a two-stage process (D2). Against expectations, no positive effect was found in OFMSW due to lactose. The best enzymatic titres were in the order D1 > OFMSW ≈ OFMSW + lactose > D2. The use of digestates from hydrogenogenic fermentation for enzyme production holds promise for waste management. It promotes energy and added-value bioproduct generation-a green alternative to common practice of management and disposal of organic wastes.

The Use of Fungi of the <em>Trichoderma </em>Genus in Anaerobic Digestion: A Review

“…Based on the results obtained, the authors concluded that the highest activity of esterase was achieved when the substrate consisted of 50% of the digestate and 50% of the food residues enriched with sawdust. In contrast, the results of the study conducted by Escamilla-Alvarado et al [93] showed that the production of holocellulases by T. reesei reached the highest values when the digestate from hydrogenogenic digestion was the carrier for the multiplication of microorganisms.…”

Valorization of Anaerobic Digestate from Biowaste to High-Value Bioproducts: A Review

“…Representative laboratory-scale studies on enzyme production from the OFMSW are listed in Table 1. [19] Polyhydroxyalkanoates (PHAs) are polyesters of hydroxyl fatty acids that are stored by a variety of bacteria as intracellular carbon and energy reserve materials [20]. PHAs can be synthesized by over 75 different bacterial genera as an intracellular carbon and energy reserve and/or as a sink for reducing redundant power consumption or electrons under unfavorable environmental and nutritional conditions [21].…”

Biohydrogen, biomethane and bioelectricity as crucial components of biorefinery of organic wastes: A review

“…Biorefinery is the sustainable processing of biomass into a spectrum of marketable products (Escamilla-Alvarado et al, 2011, 2013bFava et al, 2012;Kirtay, 2011), which means: materials, chemicals, food and feed, and energy (Figure 1). Biorefininig is a worldwide growing technology.…”