Use of novel PCR primers specific to the genes of staphylococcal enterotoxin G, H, I for the survey of Staphylococcus aureus strains isolated from food-poisoning cases and food samples in Taiwan

Chen, Tong-Rong; Chiou, Chien-Shun; Tsen, Hau‐Yang

doi:10.1016/j.ijfoodmicro.2003.10.002

Cited by 65 publications

(37 citation statements)

References 23 publications

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“…aureus possessing seg and sei genes is frequently found in healthy humans and foods [5,6,31,35,37]. However, there are incidences of food poisoning that might have been caused by S. aureus possessing both the seg and sei genes [4,5,28,36].…”

Section: Discussionmentioning

confidence: 99%

Isolation of <i>Staphylococcus aureus</i> from Raw Fish in Relation to Culture Methods

Saito

Yoshida

Kawano

et al. 2011

The Journal of Veterinary Medical Science

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ABSTRACT. Five hundred and fifty fish samples from various stages in the course of distribution in Hyogo Prefecture (209 retailed in super markets, 173 obtained from fishery cooperatives at a harbor, 91 caught by trawling and 77 caught by rod fishing) were examined for contamination with Staphylococcus aureus (S. aureus). S. aureus was detected in 41 (19.6%) of the retail fish samples and 46 (26.6%) of the samples from the fishery cooperatives. No S. aureus was isolated from the live fish (91 trawled and 77 fished by rod). With regard to the retail fish, the contamination rate of processed fish (26.0%) was significantly higher than that of unprocessed fish (14.2%). For 88 samples, the efficacy of the selective medium was compared using Baird-Parker agar and mannitol salt agar supplemented with egg yolk (MSEY agar) by the direct plate and enrichment culture methods. Using the direct culture method, the S. aureus positive rate with the Baird-Parker agar (30.7%) was significantly higher (P<0.01) than that with the MSEY agar (6.8%). The enrichment culture method remarkably raised the S. aureus detection rate. Seventy-eight (85.7%) of 91 isolates belonged to the human ecovar. Sixty-two (68.1%) of the 91 isolates had some enterotoxin genes, including 44 (48.4%) with the sea gene. These data showed that the fish were contaminated with S. aureus after landing and that Baird-Parker agar had an advantage in detecting S. aureus with a direct plate culture.

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Section: Discussionmentioning

confidence: 99%

Isolation of <i>Staphylococcus aureus</i> from Raw Fish in Relation to Culture Methods

Saito

Yoshida

Kawano

et al. 2011

The Journal of Veterinary Medical Science

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“…However, some of these studies were performed with limited numbers of strains, and sometimes controversial data are published. The association of SEG, SEH, SEI, SEO, and SEM with food poisoning, for instance, is still ill-defined (3,4).…”

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confidence: 99%

Clonal Distribution and Differential Occurrence of the Enterotoxin Gene Cluster, egc , in Carriage- versus Bacteremia-Associated Isolates of Staphylococcus aureus

et al. 2006

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The Staphylococcus aureus enterotoxin gene cluster, egc, was detected in isolates from healthy individuals and in those from patients with bacteremia. The egc genes cooccur and are slightly enriched in strains from healthy carriers (present in 63.7% of carriage-associated isolates versus 52.9% of invasive isolates; P ‫؍‬ 0.03). Multilocus sequence typing revealed that successful staphylococcal clones usually harbor the egc locus.

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“…JARRAUD et al, (2001) e NITZSCHE et al (2007, por exemplo, descrevem que cepas que possuem um desses genes, necessariamente, carrearão o outro. CHEN et al (2004) relatam que uma cepa de S. aureus pode carrear o gene seg sem necessariamente possuir o gene sei, semelhantemente ao encontrado neste estudo. THOMAS et al (2006) avaliaram 666 cepas clínicas de S. aureus, encontrando apenas uma que não apresentava o gene sei e descrevem que nessa cepa havia dois tipos de sequência de inserção posicionados na região cromossômica onde deveria estar localizado esse gene.…”

Section: Resultsunclassified

“…Vinte e duas EE já foram descritas e 10 foram envolvidas com intoxicação alimentar (EEA, EEB, EEC 1 , EEC 2 , EEC 3 , EED, EEE, EEG, EEH e EEI) (CENCI-GOGA et al, 2003). Embora a maioria dos casos e surtos de intoxicação alimentar estafilocócica seja atribuída às EE clássicas (EEA à EEE) (CHEN et al, 2004), o recente avanço dos métodos de diagnóstico permitiu a identificação de casos de intoxicação alimentar envolvendo EEG, EEH e EEI, indicando que a importância das "novas" EE pode estar sendo subestimada (CHEN et al, 2004;CENCI-GOGA et al, 2003). Em S. aureus, diversos genes codificadores de fatores de virulência estão presentes em elementos genéticos móveis, tais como ilhas de patogenicidade (SaPI), profagos e plasmídeos (JARRAUD et al, 2001).…”

Section: Introductionunclassified

Detecção de genes do cluster egc em Staphylococcus aureus isolados de alimentos de origem animal

2010

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Use of novel PCR primers specific to the genes of staphylococcal enterotoxin G, H, I for the survey of Staphylococcus aureus strains isolated from food-poisoning cases and food samples in Taiwan

Cited by 65 publications

References 23 publications

Isolation of <i>Staphylococcus aureus</i> from Raw Fish in Relation to Culture Methods

Isolation of <i>Staphylococcus aureus</i> from Raw Fish in Relation to Culture Methods

Clonal Distribution and Differential Occurrence of the Enterotoxin Gene Cluster, egc , in Carriage- versus Bacteremia-Associated Isolates of Staphylococcus aureus

Detecção de genes do cluster egc em Staphylococcus aureus isolados de alimentos de origem animal

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