Use of NMR metabolomic plasma profiling methodologies to identify illicit growth-promoting administrations

Graham, Stewart F.; Ruiz‐Aracama, Ainhoa; Lommen, Arjen; Cannizzo, Francesca Tiziana; Biolatti, B.; Elliott, Christopher T.; Mooney, Mark

doi:10.1007/s00216-012-5815-z

Cited by 19 publications

(8 citation statements)

References 37 publications

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“…The signals from the proteins can be suppressed using specific NMR experiments. Typically, one-dimensional (1D) NMR spectra are recorded using a 'T2 filter' procedure, which attenuates the signal from macromolecules such as proteins and lipids [52,53]. One potential drawback of not removing macromolecules from the sample is that it can affect the quantification of small molecules that interact with the macromolecules [54].…”

Section: Nmr Spectroscopy In Metabolomicsmentioning

confidence: 99%

The utility of nuclear magnetic resonance spectroscopy in assisted reproduction

et al. 2020

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Infertility affects approximately 15–20% of individuals of reproductive age worldwide. Over the last 40 years, assisted reproductive technology (ART) has helped millions of childless couples. However, ART is limited by a low success rate and risk of multiple gestations. Devising methods for selecting the best gamete or embryo that increases the ART success rate and prevention of multiple gestation has become one of the key goals in ART today. Special emphasis has been placed on the development of non-invasive approaches, which do not require perturbing the embryonic cells, as the current morphology-based embryo selection approach has shortcomings in predicting the implantation potential of embryos. An observed association between embryo metabolism and viability has prompted researchers to develop metabolomics-based biomarkers. Nuclear magnetic resonance (NMR) spectroscopy provides a non-invasive approach for the metabolic profiling of tissues, gametes and embryos, with the key advantage of having a minimal sample preparation procedure. Using NMR spectroscopy, biologically important molecules can be identified and quantified in intact cells, extracts or secretomes. This, in turn, helps to map out the active metabolic pathways in a system. The present review covers the contribution of NMR spectroscopy in assisted reproduction at various stages of the process.

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Section: Nmr Spectroscopy In Metabolomicsmentioning

confidence: 99%

The utility of nuclear magnetic resonance spectroscopy in assisted reproduction

et al. 2020

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“…Another study by Graham et al (2012) focused on bovine plasma samples in order to highlight the presence of illicit growth-promoting agents. These products are illegally used to improve feed efficiency, increasing animal weight gain.…”

Section: Using Metabolomics To Identify the Geographical Origin Of Fomentioning

confidence: 99%

Using metabolomics to describe food in detail

Capozzi

Trimigno

2015

Metabolomics as a Tool in Nutrition Research

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“…Nevertheless, some general conclusions can be drawn. In three studies, a decrease in urine and serum creatine was reported to be related to the administration of clenbuterol and the steroids, estradiol, dexamethasone, prednisolone 102,110,113 . The latter reports also an increase in creatinine after steroid administration, which generally is related also to a lean mass increase 124 .…”

Section: A Metabolomics Approach For Hormone Screening?mentioning

confidence: 98%

A role for metabolomics in the antidoping toolbox?

Narduzzi

Audran²,

Bizec

et al. 2020

Drug Testing and Analysis

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Evidence of the continuous rise of novel doping agents and novel doping strategies calls for the development of more accurate multi‐target screening methods. Direct multi‐target screening approaches are restricted to the targeted substances and their turnover. The development of effective “indirect” screening methods requires a priori a deep understanding of the metabolism of the substance. The biological passport has been demonstrated to be very effective, but it is limited to about 20 indirect parameters. The standard antidoping analytical methods are hence targeted and do not aim directly to identify unknown substances. Also, the detection of doping agents is limited by the excretion of the substance. This study considers metabolomics for the screening of performance enhancing hormone abuse by athletes, based on the following pieces of evidence: (1) hormones have a strong influence on human metabolism, changing several parameters in many tissues, organs, and bio‐fluids; (2) metabolomics has been demonstrated to be a very accurate tool to depict the metabolic status of several organisms, tissues, and for several human diseases, including hormone deficiencies; (3) metabolomics has been demonstrated to be able to distinguish hormone‐treated animals from controls in many species, without the need for a priori knowledge of the metabolism for the specific substance. The literature shows that metabolomics could be an appropriate tool to detect hormone abuse, keeping in mind the strength and the limitation of such an approach.

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Use of NMR metabolomic plasma profiling methodologies to identify illicit growth-promoting administrations

Cited by 19 publications

References 37 publications

The utility of nuclear magnetic resonance spectroscopy in assisted reproduction

The utility of nuclear magnetic resonance spectroscopy in assisted reproduction

Using metabolomics to describe food in detail

A role for metabolomics in the antidoping toolbox?

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