Use of Next-Generation Sequencing for Diagnosis of West Nile Virus Infection in Patient Returning to Belgium from Hungary

Smolders, David; Naesens, Reinout; Bruynseels, Peggy; Lagrou, Katrien; Matthijnssens, Jelle; Ranst, Marc Van

doi:10.3201/eid2412.180494

Cited by 15 publications

(7 citation statements)

References 9 publications

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“…One Western European sequence, which was imported to Belgium from Hungary, was grouped to the Balkanian subclade. This offers a more precise evolutionary origin regarding this imported case than previously published [36] (Figure 4). The progenitor strains were segregated outside the two groups, advisably indicating the order of WNV strains emergence in time and route of introduction to Hungary.…”

Section: Phylogenetic Analysismentioning

confidence: 56%

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Multi-Approach Investigation Regarding the West Nile Virus Situation in Hungary, 2018

Zana

Erdélyi

Nagy

et al. 2020

Viruses

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The West Nile virus is endemic in multiple European countries and responsible for several epidemics throughout the European region. Its evolution into local or even widespread epidemics is driven by multiple factors from genetic diversification of the virus to environmental conditions. The year of 2018 was characterized by an extraordinary increase in human and animal cases in the Central-Eastern European region, including Hungary. In a collaborative effort, we summarized and analyzed the genetic and serologic data of WNV infections from multiple Hungarian public health institutions, universities, and private organizations. We compared human and veterinary serologic data, along with NS5 and NS3 gene sequence data through 2018. Wild birds were excellent indicator species for WNV circulation in each year. Our efforts resulted in documenting the presence of multiple phylogenetic subclades with Balkans and Western-European progenitor sequences of WNV circulating among human and animal populations in Hungary prior to and during the 2018 epidemic. Supported by our sequence and phylogenetic data, the epidemic of 2018 was not caused by recently introduced WNV strains. Unfortunately, Hungary has no country-wide integrated surveillance system which would enable the analysis of related conditions and provide a comprehensive epidemiological picture. The One Health approach, involving multiple institutions and experts, should be implemented in order to fully understand ecological background factors driving the evolution of future epidemics.

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Section: Phylogenetic Analysismentioning

confidence: 56%

“…Samples from 2018, Hungary, are highlighted with red color. Note MH021189 as an imported case from Hungary to Belgium[36].…”

mentioning

confidence: 99%

Multi-Approach Investigation Regarding the West Nile Virus Situation in Hungary, 2018

Zana

Erdélyi

Nagy

et al. 2020

Viruses

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“…Recent studies have shown that NGS has been used for the diagnosis of various infectious diseases such as bacteria, [13][14][15] fungi, 16 mycoplasma, 17 chlamydia, 18 viruses, [19][20][21] and parasites. 22 These attempts suggest that NGS can be used as an alternative diagnostic or complementary method to help clinicians make appropriate decisions.…”

Section: Discussionmentioning

confidence: 99%

<p>Application of NGS in Diagnosis of Tuberculous Pleurisy with Multiple Negative Tests: A Case Report</p>

Wu¹,

Wei²,

Yu³

2020

IDR

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Background: Tuberculous pleurisy is inflammation caused by direct infection of Mycobacterium tuberculosis (MTB) and/or delayed allergic reaction of the pleura to MTB thallus components. The diagnosis of tuberculous pleurisy is mainly confirmed by bacterial culture, smear staining or histopathology, but has some clinical limitations. Next-generation sequencing (NGS), as a new diagnostic technology, has good application prospects in the diagnosis of tuberculous pleurisy. Case Presentation: A patient admitted with right pleural effusion and pneumonia was actively treated with anti-infection, anti-inflammatory and symptomatic support while various etiological tests of right pleural effusion were improved. However, all the etiological tests for MTB infection were negative. At this time, the patient's condition worsened and pleural effusion also appeared on the left side. In order to clarify the cause of the disease as soon as possible and prevent the disease from worsening again, the left and right pleural effusions of the patient were sent for NGS testing. The test results suggested MTB infection, which finally clarified the diagnosis of tuberculous pleurisy, and the next treatment plan of the patient was timely adjusted. Conclusion: NGS is instructive in the diagnosis of tuberculous pleurisy when various conventional tests and imaging methods fail.

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“…In recent years, mNGS has been successfully applied in the detection of pathogens in blood, cerebrospinal fluid (CSF), bronchoalveolar lavage (BAL), urine, and other samples. [7][8][9] Although mNGS is superior over traditional methods due to its wide coverage, its use is also limited by high cost and low specificity. Recent studies suggested that the efficacy of mNGS in the pathogenic diagnosis of infection is limited in immunocompetent patients.…”

Section: Introductionmentioning

confidence: 99%

Metagenomic Next-Generation Sequencing in the Diagnosis of Infectious Fever During Myelosuppression Among Pediatric Patients with Hematological and Neoplastic Diseases

Fu¹,

Zhu²,

Cao³

et al. 2022

IDR

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Purpose To analyze the contribution of metagenomic next-generation sequencing (mNGS) in the guidance of clinical treatment and outcomes of infection during myelosuppression among children with hematological and neoplastic diseases. Patients and Methods The clinical data and results of mNGS assay of febrile patients suspected of infection were retrospectively collected. The characteristics of pathogenic microorganisms and clinical course of myelosuppressed children with hematological diseases were summarized. Results Our study included 70 patients (45 males) with a median age of 5 years (range: 0.5 to 13 y). During the study period, there were 96 events of suspected infection. According to comprehensive clinical diagnosis, 73 blood infections, 43 pneumonia and 2 urinary tract infections occurred. The positive rate of mNGS was significantly higher than that of traditional microbial detection (83.3% vs 17.7%). The main pathogens detected by mNGS were Pseudomonas aeruginosa, Acinetobacter , human herpesvirus, Candida and Aspergillus . The average duration of fever was 4.9 days and 11.6 days ( P < 0.05), and the average cost of anti-infection treatment was RMB ¥28,077 and 39,898 ( P < 0.05) among children received mNGS within 48 hours and more than 48 hours after the onset of infection symptoms. Conclusion mNGS contributes to clinical management of children with infection during myelosuppression, especially among patients with negative traditional microbial detection. Early implementation of mNGS in children with symptoms has a tendency to reduce the time of infection, fever and the cost of treatment.

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Use of Next-Generation Sequencing for Diagnosis of West Nile Virus Infection in Patient Returning to Belgium from Hungary

Cited by 15 publications

References 9 publications

Multi-Approach Investigation Regarding the West Nile Virus Situation in Hungary, 2018

Multi-Approach Investigation Regarding the West Nile Virus Situation in Hungary, 2018

<p>Application of NGS in Diagnosis of Tuberculous Pleurisy with Multiple Negative Tests: A Case Report</p>

Metagenomic Next-Generation Sequencing in the Diagnosis of Infectious Fever During Myelosuppression Among Pediatric Patients with Hematological and Neoplastic Diseases

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