Use of Near-Infrared Spectroscopy for Screening the Individual and Total Glucosinolate Contents in Indian Mustard Seed (<i>Brassica juncea</i> L. Czern. &amp; Coss.)

Font, Rafael; Rı́o, M. Del; Fernández‐Martínez, José M.; Bailón, Antonio de Haro

doi:10.1021/jf0307649

Cited by 48 publications

(45 citation statements)

References 15 publications

(23 reference statements)

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“…With this global calibration, the prediction is less certain, but it can be used with unknown samples or those that have been contaminated or mixed with seeds from another species. Our results were similar to those obtained by Daun et al (1994) and Font et al (2004) although others have developed calibrations with much higher correlations (Petisco et al, 2010;Velasco and Becker, 1998a).…”

Section: Glucosinolates Calibrationsupporting

confidence: 91%

Development of near-infrared spectroscopy calibrations to measure quality characteristics in intact Brassicaceae germplasm

Oblath

Isbell

Berhow

et al. 2016

Industrial Crops and Products

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a b s t r a c tDetermining seed quality parameters is an integral part of cultivar improvement and germplasm screening. However, quality tests are often time consuming, seed destructive, and can require large seed samples. This study describes the development of near-infrared spectroscopy (NIRS) calibrations to measure moisture, oil, fatty acid profile, nitrogen, glucosinolate, and chlorophyll content in six species from the Brassicaceae family. Rapeseed and similar oilseeds are potential feedstocks for producing hydrotreated renewable jet fuel. Screening samples with NIRS would allow cultivars with desirable characteristics to be quickly identified. A total of 367 samples of six species (Brassica napus, Brassica carinata, Brassica juncea, Brassica rapa, Sinapis alba, and Camelina sativa) were scanned with NIRS. Global calibrations for all six species were developed using modified partial least squares regression with reference values obtained through wet chemistry techniques. Comparing predicted values to reference data, the coefficients of determination (r 2 ) and ratios of performance to deviation (RPD) varied, with some calibrations performing better than others. The calibration equations for seed oil content (r 2 = 0.98, RPD = 7.3) and nitrogen (r 2 = 0.98, RPD = 5.3) performed very well while the equations for seed moisture (r 2 = 0.93, RPD = 3.8) and total glucosinolate content (r 2 = 0.92, RPD = 2.3) were more qualitative. Large variation was observed for chlorophyll content (0-390 mg/kg) so two calibration equations were developed, one for the higher and one for the lower range of values. When combined, these calibrations also showed very good performance (r 2 = 0.99, RPD = 14). The performance of the calibrations for the fatty acids was more varied, with some performing very well, such as the calibration for C 18:3 (r 2 = 0.99, RPD = 9.9), and others, such as C 22:0 (r 2 = 0.69, RPD = 1.9), showing poor correlation. (E.A. Oblath), terry.isbell@ars.usda.gov (T.A. Isbell), mark.berhow@ars.usda.gov (M.A. Berhow), brett.allen@ars.usda.gov (B. Allen), david.archer@ars.usda.gov (D. Archer), jbrown@uidaho.edu (J. Brown), russ.gesch@ars.usda.gov (R.W. Gesch),

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Section: Glucosinolates Calibrationsupporting

confidence: 91%

Development of near-infrared spectroscopy calibrations to measure quality characteristics in intact Brassicaceae germplasm

Oblath

Isbell

Berhow

et al. 2016

Industrial Crops and Products

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“…a 1-Ethyl-2-hydroxyethyl DS-GSLs: glucosisautricin or 2-hydroxy-2-methylpropyl DS-GSLs: glucoconringin (assigned unknown compound) [6]; b hydroxy-3,4,5-trimethoxybenzoyl DSGSLs (presumed unknown compound) [7] juncea germplasm [13,28]. The accession K046145 exhibited the highest sinigrin content (9.38 mg/g DW, purple) with a total DS-GSL content (16.4 mg/g DW).…”

Section: Fig 1 Mass Spectra and Interpretation Of Unknown Ds-gsls Inmentioning

confidence: 99%

Identification and quantification of glucosinolates in Korean leaf mustard germplasm (Brassica juncea var. integrifolia) by liquid chromatography–electrospray ionization/tandem mass spectrometry

Kim

Baek

et al. 2016

Eur Food Res Technol

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“…Several intraspecific studies have documented genetic variation for glucosinolate type and concentration in Brassica species (Louda and Rodman, 1983;Kliebenstein et al, 2001;Fahey et al, 2001;Castro et al, 2004;Font et al, 2004;Charron and Sams, 2004). All of the different cruciferous vegetable types contain glucobrassicin, and most contain substantial amounts of sinigrin.…”

Section: Individual Glucosinolates and Variation In Brassica Rapamentioning

confidence: 99%

Genetic Variation in Glucosinolate Content Within Brassica Rapa Vegetables

He¹,

Lou²,

Bonnema³

et al. 2012

Acta Hortic.

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Glucosinolates (GSs) were analyzed in 56 accessions of Brassica rapa grown in the greenhouse. Eight different glucosinolates were identified in the Brassica rapa group. They are the aliphatic glucosinolates progoitrin (PRO), gluconapin (NAP), glucobrassicanapin (GBN), the indolyl glucosinolates 4-hydroxyglucobrassicin (4OH), glucobrassicin (GBC), 4-methoxyglucobrassicin (4ME), neoglucobrassicin (NEO) and the aromatic glucosinolate gluconasturtiin (NAS). Gluconapin, glucobrassicanapin, progoitrin and gluconasturtiin are the most abundant GSs in the Brassica rapa, but there is considerable variation in content among accessions. The total glucosinolate contents in Brassica rapa group varied substantially between the different accessions. The highest amount of GSs (361 mol/100 g FW) was observed in leaves of vegetable turnip, followed by rapid cycling and yellow sarson, with the amount of 200 and 178 mol/100 g FW respectively. Whereas the lowest GSs content was found in turnip greens (20.8 mol/100 g FW) and Wutacai (22.6 mol/100 g FW). The total aliphatic GSs proportion varied from 50 to 90% of the total GS, while generally the content of indolyl glucosinoloates, especially 4OH glucobrassicin and neoglucobrassicin is low. Gluconasturtiin was found in relatively high concentrations in komastsuna (14.6 μmol/100 g FW), yellow sarson (7.1 μmol/ 100 g FW) and constitutes as much as 24% of the total amount of glucosinolates. Relatively high amounts of gluconapin (281 mol/100 g FW) and glucobrassicanapin (60.0 mol/100 g FW) were observed in the leaves of vegetable turnip. Compared with the Brassica oleracea group, Brassica rapa lacks glucoraphanin and sinigrin but contains gluconapin and glucobrassicanapin. Variations in glucosinolate content among genotypes suggest differences in their health-promoting properties and the opportunity for enhancement of their levels through breeding or genetic modification.

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Use of Near-Infrared Spectroscopy for Screening the Individual and Total Glucosinolate Contents in Indian Mustard Seed (Brassica juncea L. Czern. & Coss.)

Cited by 48 publications

References 15 publications

Development of near-infrared spectroscopy calibrations to measure quality characteristics in intact Brassicaceae germplasm

Development of near-infrared spectroscopy calibrations to measure quality characteristics in intact Brassicaceae germplasm

Identification and quantification of glucosinolates in Korean leaf mustard germplasm (Brassica juncea var. integrifolia) by liquid chromatography–electrospray ionization/tandem mass spectrometry

Genetic Variation in Glucosinolate Content Within Brassica Rapa Vegetables

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