Use of multivariate analysis to evaluate the effect of sucrose on in vitro cassava conservation

Vieira, Lívia de Jesus; Santana, Jrf de; Vidal, AM; Alves, Aac; Souza, A. da S.; Silva, Cal da; Souza, F. C. de

doi:10.5897/ajb2014.14210

Cited by 2 publications

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“…In contrast, Vieira et al (2015) were able to fit an optimal SUC concentration that enabled the in vitro viability of Manihot esculenta plants. Hence, as reported by Souza et al (2020), for each species there may be different responses when sucrose is added to the culture medium.…”

Section: /8mentioning

confidence: 93%

Effects of different doses of paclobutrazol and sucrose in the minimum in vitro growth of wild species of Manihot

Sá

Silveira

Santos

et al. 2021

Agraria

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In vitro conservation, under minimal growth conditions, allows prolonging the periods between subcultures without affecting plant viability, besides reducing the contamination risk and the space required for cultivation. Experiments were conducted by using 8S as a basic culture medium, supplemented with combinations of five concentrations of paclobutrazol (PBZ -0; 0.10; 0.20; 0.30 and 0.40 mg L -1 ) and sucrose (SUC -0; 5; 10; 15; and 20 g L -1 ), resulting in 25 treatments, applied on three wild species: Manihot violaceae, M. flabellifolia and M. pseudoglaziovii. After 120 days of cultivation, the evaluated variables, namely plant height (cm) and the numbers of mini-cuttings with 1 cm, roots, shoots, green and senescent leaves, allowed verifying that, for the species M. pseudoglaziovii, the interaction of the 0.1 mg L -1 PBZ and 5 g L -1 SUC doses revealed a significant effect on the in vitro conservation. While for M. violacea, the combination of 10 g L -1 SUC and 0.2 mg L -1 PBZ is the most suitable for its conservation. Lastly, for M. flabelifolia, the concentrations used of SUC and PBZ did not prove efficient for in vitro conservation of the species.

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Section: /8mentioning

confidence: 93%

Effects of different doses of paclobutrazol and sucrose in the minimum in vitro growth of wild species of Manihot

Sá

Silveira

Santos

et al. 2021

Agraria

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“…Existem poucos estudos in vitro e ex vitro considerando diferentes concentrações de sacarose no processo de micropropagação de mandioca, a maioria dos estudos tem enfoque em alterações na concentração de sacarose para fins de conservação (VIEIRA et al, 2015;SINTIM;AKROMAH, 2015). A influência de luz e diferentes concentrações de sacarose no meio foram testadas em porta-enxertos de pereira (LEITE et al, 2000), mirtilo (DAMIANI; SCHUCH, 2008), tomilho (BANDEIRA et al, 2008) e ginseng brasileiro (MALDANER et al, 2006).…”

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Light Ans Sucrose Influence on the in Vitro Growth of Cassava

Cardoso

Araujo

Silva

et al. 2018

Nucleus

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RESUMO:A mandioca é a única espécie do gênero Manihot comercialmente produzida. A produção de mudas micropropagadas possui limitações, e entre estas está o alto custo com energia utilizadas nas salas de crescimento, e na taxa de sobrevivência das plantas na fase de aclimatização, porém a cultura de tecidos é uma ferramenta de multiplicação rápida e obtenção de plantas sadias (cultura de meristema), pois o método de propagação vegetativa acarreta problemas fitossanitários como disseminação de patógenos. Uma alternativa para diminuir gastos dessa técnica seria a redução de sacarose ao meio e indução de enraizamento sob luz natural em casa de vegetação. Sendo assim, o objetivo desse trabalho foi avaliar a influência da luz e sacarose na sobrevivência e desenvolvimento de mudas de mandioca micropropagada. As variedades BRS Formosa, Lagoão e BRS Verdinha, cultivadas em meio MS acrescido de diferentes concentrações de sacarose (10; 20 e 30 g.L -1), e após 30 dias em sala de crescimento, os frascos foram incubados em sala de crescimento e casa de vegetação por 15 dias. Em cultivos envolvendo diferentes tipos de luz, BRS Formosa e Lagoão, alcançaram melhores médias sob luz natural e altas concentrações de sacarose, enquanto que BRS Verdinha obteve melhor desenvolvimento em luz natural e baixa concentração de sacarose. Palavras-chave:Manihot esculenta. Carboidratos. Micropropagação. Aclimatização. LIGHT ANS SUCROSE INFLUENCE ON THE IN VITRO GROWTH OF CASSAVA SUMMARY:Cassava is the only species of the genus Manihot commercially produced. The production of micropropagated seedlings has limitations, and among these is the high-energy cost used in growing rooms, and the survival rate of the plants in the acclimatization phase, but the tissue culture is a tool of rapid multiplication and obtaining of plants (Meristem culture), because the vegetative propagation method leads to phytosanitary problems such as the dissemination of pathogens. An alternative to reduce costs of this technique would be the reduction of sucrose to the medium and induction of rooting under natural light in greenhouse. Therefore, the objective of this work was to evaluate the influence of light and sucrose on the survival and development of micropropagated cassava seedlings. The BRS Formosa, Lagoão and BRS Verdinha varieties, cultivated in MS medium with different concentrations of sucrose (10, 20 and 30 gL-1), and after 30 days in the growth room, the flasks were incubated in room growth and greenhouse for 15 days. In crops involving different types of light, BRS Formosa and Lagoão had better averages under natural light and high concentrations of sucrose, whereas BRS Verdinha had a better development in natural light and low concentration of sucrose

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Use of multivariate analysis to evaluate the effect of sucrose on in vitro cassava conservation

Abstract: The aim of this work was to evaluate the effect of sucrose to reduce the in vitro growth of cassava plants using multivariate statistical tests. Cassava conservation has a relevant role as an auxiliary strategy for preservation and genetic breeding.

Cited by 2 publications

References 16 publications

Effects of different doses of paclobutrazol and sucrose in the minimum in vitro growth of wild species of Manihot

Effects of different doses of paclobutrazol and sucrose in the minimum in vitro growth of wild species of Manihot

Light Ans Sucrose Influence on the in Vitro Growth of Cassava

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