Use of monoclonal antibodies to human immunoglobulin M in "capture" assays for measles and rubella immunoglobulin M

Forghani, Bagher; Myoraku, Carolyn K.; Schmidt, Nathalie J.

doi:10.1128/jcm.18.3.652-657.1983

Cited by 17 publications

(13 citation statements)

References 11 publications

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“…The sensitivity of these assays depends upon a high proportion of virus-specific IgM antibody in sera, since other IgM-class antibody, including RF, can reduce the sensitivity of antibody capture EIAs by competing with virus-specific IgM for binding sites on the solid phase. RF bound to the solid phase has been shown to produce false-positive results in a number of different assays (11,15,20,32). This may occur as a result of RF binding enzyme-labeled antiviral antibodies directly or by complexing with serum antiviral IgG antibodies which bind viral antigen (11).…”

Section: Discussionmentioning

confidence: 99%

“…This may occur as a result of RF binding enzyme-labeled antiviral antibodies directly or by complexing with serum antiviral IgG antibodies which bind viral antigen (11). RF interference has therefore necessitated the removal of IgG antibody by treatment with either staphylococcal protein A, aggregated IgG, or IgG-coated latex particles prior to testing (4,11,29,32). Additional approaches for improving specificity have included the use of purified viral antigen to eliminate false-positive IgM results obtained with crude antigens (11,28,29) antigen to allow identification of false-positive reactions in antibody capture assays (11).…”

Section: Discussionmentioning

confidence: 99%

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Characteristics of different solid-phase immunoassay formats for the measurement of BK virus immunoglobulin M in sera of patients on renal dialysis or with kidney allografts

1989

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Solid-phase immunoglobulin M (IgM) antigen capture enzyme immunoassay (AgCEIA) and antibody capture enzyme immunoassay (AbCEIA) were developed for the diagnosis of BK virus (BKV) infections. Of 37 serum samples from renal allograft recipients, 15 were positive for BKV IgM antibody by either AgCEIA, AbCEIA, or antigen capture radioimmunoassay. False-positive IgM results were observed in the AgCEIA in the presence of high levels of BKV IgG antibody (titers 1:51,200), when rheumatoid factor (RF) titers were-1:20, or in the presence of high levels of RF (titers-1:10,240) when BKV hemagglutination inhibition titers exceeded 1:40. False-positives due to RF could be eliminated by treatment of sera with anti-human IgG antisera or IgG-coated latex particles. The presence of RF did not, however, produce false-positive results in the AbCEIA. Both AgCEIA and AbCEIA were specific for BKV IgM antibody, as 14 serum samples containing either JC papovavirus, cytomegalovirus, rubella virus, hepatitis A virus, or hepatitis B virus core IgM antibody were negative in both EIAs. Comparison of results obtained for 37 serum samples revealed 14 positive by radioimmunoassay and 11 positive by both AgCEIA and AbCEIA. Both EIAs detected BKV IgM antibody in sera of renal allograft patients and patients on renal dialysis who had reactivated BKV infections persisting for several months after transplantation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Characteristics of different solid-phase immunoassay formats for the measurement of BK virus immunoglobulin M in sera of patients on renal dialysis or with kidney allografts

1989

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“…Diagnosis of measles may be confirmed by virus isolation, by the demonstration of a significant increase in specific immunoglobulin G (IgG) titers, or by the detection of anti-measles virus (MV) IgM antibodies by using radioimmunoassays (2,18,37), enzyme-linked immunosorbent assays (ELISAs) (27,34), and direct or indirect fluorescence-antibody techniques (20,24). MV IgM appears at the same time as rash (12,21,34) and can be detected 3 days after the onset of rash in most individuals (29,32). IgM peaks on days 7 to 10 and wanes within weeks (28).…”

mentioning

confidence: 99%

“…Since IgM is transient, the demonstration of spe-cific IgM corresponds to a recent primary measles infection (14,21). A single serum specimen collected at the appropriate time (14) is now generally accepted to be sufficient to diagnose measles (10,12,14,17,26,32,34,37).…”

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confidence: 99%

Evaluation of Hemagglutinin Protein-Specific Immunoglobulin M for Diagnosis of Measles by an Enzyme-Linked Immunosorbent Assay Based on Recombinant Protein Produced in a High-Efficiency Mammalian Expression System

et al. 1998

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Recombinant hemagglutinin (H) of the measles virus (MV) expressed in a mammalian high-expression system based on the Semliki Forest virus replicon was used in an enzyme-linked immunosorbent assay (ELISA) for the detection of specific immunoglobulin M (IgM) and IgG in patients with acute-phase measles. One hundred twelve serum specimens from 70 patients with measles were analyzed. Case definition was based on a commercial IgM ELISA that utilizes MV-infected cells (MV-ELISA) (Enzygnost; Behring Diagnostics); the clinical criteria of the Centers for Disease Control and Prevention (Atlanta, Ga.); and/or the increase in hemagglutinin test titers, neutralization test titers, and levels of MV-specific IgG whenever paired sera were available. The initial time courses of the IgM signal after the onset of rash are similar in the H- and MV-ELISAs. On days 0 to 19, both ELISAs detected IgM in 67 of 68 (98.5%) sera. Average maximal levels of IgM seem to persist, however, about 10 days longer in the MV-ELISA (up to day 25) than in the H-ELISA (day 15). From days 20 to 29 and 30 to 59, the H-ELISA detected only 64.3 (9 of 14) and 19.2% (5 of 26), respectively, of sera that were IgM positive by MV-ELISA. At least up to day 30, the performance of the H-ELISA seemed to be similar to that reported for commercial ELISAs based on whole MV. Our results demonstrate that MV H-specific IgM can be used to diagnose most measles cases from a single serum specimen collected within 19 days after the onset of rash and that the recombinant protein used in this study is suitable for this purpose.

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“…Indirect EIAs-in which viral antigen is coated directly onto the solid phase-are particularly popular because of their sensitivity and simplicity of design (9), but they have also been criticized for their susceptibility to nonspecific reactions (11). To address these problems, reverse or capture EIAs-in which the solid phase is first coated with an anti-human immunoglobulin class-specific antibody and then with patient serum-have been developed to detect specific IgM antibodies (10,16,18). These assays are noted for their enhanced specificity and resistance to interference from rheumatoid factor (RF) (27).…”

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confidence: 99%

Evaluation of monoclonal antibody-based capture enzyme immunoassays for detection of specific antibodies to measles virus

et al. 1991

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Monoclonal antibodies to the hemagglutinin protein, fusion protein, phosphoprotein, matrix protein, and nucleoprotein of measles virus were evaluated as detector antibodies in capture enzyme immunoassays (EIAs) for the detection of specific serum immunoglobulin G (IgG), IgA, and IgM antibodies to measles virus. A pool of monoclonal antibodies to hemagglutinin protein and nucleoprotein proved optimal and was further evaluated. Specific IgM was detected in 97% of adolescents with clinical measles, 97% of infants 3 weeks postvaccination, and <1% of normal serum specimens. Specffic IgA antibodies were found in 97% of adolescents with clinical measles, 97% of infants 3 weeks postvaccination, and <1% of normal serum specimens. Specific IgA antibodies were found in 97% of clinical measles cases and vaccinees, in 26% of healthy persons, and in 36% of infants 8 months postvaccination; consequently, IgA antibodies were not a useful indicator of recent measles infection. A significant increase in IgG antibodies between paired specimens was detected in 92% of clinical cases and all vaccinees. Only 59% of infant specimens had persistent IgG antibodies as detected by capture EIA at 8 months postvaccination, whereas all specimens had antibodies as detected by hemagglutination inhibition and plaque neutralization. An alternative indirect EIA, in which antigen was directly absorbed to the solid phase, was more sensitive than the capture design, detecting IgG antibodies in all infants postvaccination. When standardized with a microneutralization assay for the detection of persistent antibodies, the indirect IgG EIA gave predictive values for positive and negative tests exceeding 90%. Our capture IgM and indirect IgG EIAs provide a practical combination of serologic tests for the determination of acute measles virus infection and past exposure to measles virus or vaccine, respectively. 1466 on July 6, 2020 by guest

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Use of monoclonal antibodies to human immunoglobulin M in "capture" assays for measles and rubella immunoglobulin M

Cited by 17 publications

References 11 publications

Characteristics of different solid-phase immunoassay formats for the measurement of BK virus immunoglobulin M in sera of patients on renal dialysis or with kidney allografts

Characteristics of different solid-phase immunoassay formats for the measurement of BK virus immunoglobulin M in sera of patients on renal dialysis or with kidney allografts

Evaluation of Hemagglutinin Protein-Specific Immunoglobulin M for Diagnosis of Measles by an Enzyme-Linked Immunosorbent Assay Based on Recombinant Protein Produced in a High-Efficiency Mammalian Expression System

Evaluation of monoclonal antibody-based capture enzyme immunoassays for detection of specific antibodies to measles virus

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