Use of microsatellites and a combinatorial optimization approach in the acquisition of gilthead seabream (Sparus aurata L.) broodstocks for hatcheries

Borrell, Yaisel J.; Carleos, Carlos; Asturiano, Juan F.; Bernardo, David; Vázquez, Esther; Corral, Norberto; Sánchez, José A.; Blanco, Gloria

doi:10.1016/j.aquaculture.2007.04.055

Cited by 27 publications

(26 citation statements)

References 39 publications

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“…Observed deviations from Hardy-Weinberg equilibrium in wild and farmed southern populations reflect non-random mating and population mixing. Non-random breeding and low effective population size is a typical scenario in hatcheries where nongenetic management occurs (Borrell et al 2007, Castro et al 2007, Karaiskou et al 2009). This interpretation is further supported by a lower average number of alleles per locus and allelic richness in reared versus wild populations, something also observed for other aquaculture species such as Atlantic salmon (e.g.…”

Section: Discussionmentioning

confidence: 99%

Morphological and molecular differentiation of wild and farmed gilthead sea bream Sparus aurata: implications for management

Šegvić-Bubić¹,

Talijančić²,

Grubišić³

et al. 2014

Aquacult. Environ. Interact.

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A combination of molecular and morphological characteristics was used to investigate populations of wild and farmed gilthead sea bream Sparus aurata from the largest Croatian shellfish production area, Mali Ston Bay. Identification of farmed escapees was performed in the wild in order to evaluate and compare the detection sensitivity of molecular and morphological tools. Using a traditional set of measurements and a truss network system, morphometric trait analysis of gilthead sea bream showed clear body shape differences between individuals from the wild and farmed populations. Microsatellite markers only revealed weak neutral genetic differentiation between farmed and wild samples from Mali Ston Bay (Wright's F ST = 0.019, p > 0.05; Jost's D EST = 0.022), despite the fact that the farmed gilthead sea bream were of Atlantic origin. Using assignment tests, morphological (16%) and molecular (25%) analysis identified farmed escapees in the samples of wild gilthead sea bream in the vicinity of aquaculture sites. Morphological data showed good potential for distinguishing recent escapees in wild populations, since these characteristics are affected by the environment. This study demonstrates the presence of escapees in the local wild population in this region, and suggests the potential for genetic interaction.

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Section: Discussionmentioning

confidence: 99%

Morphological and molecular differentiation of wild and farmed gilthead sea bream Sparus aurata: implications for management

Šegvić-Bubić¹,

Talijančić²,

Grubišić³

et al. 2014

Aquacult. Environ. Interact.

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“…These results were expected because the broodstocks were formed from the identical ancestral, from the Paraná River (foundation effect), and identical individuals were introduced from the Tietê and Grande rivers. Additional factors, such as the raising system (i.e., a small group of broodstocks, sexual proportions, reproductive dominance, endogamy, high variations among individuals during reproduction) and fluctuation in population size over time, can also be influenced by the homogeneity of current broodstocks (CAMERON-BROWN et al, 2005;BORREL et al, 2007;FOPP-BAYAT et al, 2010;LOPERA-BARRERO et al, 2010b;POVH et al, 2010).…”

Section: Genetic Structurementioning

confidence: 99%

“…The establishment of broodstocks for restocking programs requires a genetic variability analysis because it determines the best genetic pool from the WPs (BORREL et al, 2007), offers an important base to formulate reproductive strategies (LOPERA-BARRERO, 2009) and minimizes adaptation to captivity (WILLIAMS; HOFFMAN, 2009). The use of related individuals reduces the effective size of the population and endogamic depression (GONZÁLEZ-WANGUEMERT et al, 2012).…”

Section: Genetic Conservationmentioning

confidence: 99%

“…Although these programs have been performed over several decades, studies of genetic and environmental efficiencies are necessary (AGOSTINHO et al, 2005;POVH et al, 2010). However, many programs have been unsuccessful because of the inappropriate management of broodstocks (BSs) (BORREL et al, 2007;LOPERA-BARRERO, 2009) and the non-monitoring of WPs. Thus, the genetic evaluation of BSs to participate in restocking programs for WPs must continuously occur (WARD, 2006).…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity of pacu for restocking programs in the Tietê and Grande rivers, Brazil

Ribeiro

Lopera-Barrero

Santos³

et al. 2015

Sem. Ci. Agr.

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Piaractus mesopotamicus is a tropical fish under threat because of declines in the number of wild populations. Restocking programs have been developed as a conservation method, and the genetic monitoring of populations and broodstocks is important to ensure the viability of such programs. The objective of the current unprecedented experiment was to evaluate the genetic diversity of P. mesopotamicus wild populations (WPs) and broodstocks (BSs) using microsatellites markers for supplying the restocking programs in the Tietê and Grande rivers. Six microsatellite loci were amplified using the DNA obtained from the caudal fin of 279 adult fish. The observed intrapopulation genetic variability was high with mean heterozygosity ranging from 0.203 to 0.833. The number of alleles per locus ranged from three (loci Pme28 and Pme32) to thirteen (loci Pme4, Pme5 and Pme14), and there were allele differences between WPsxWPs and WPsxBSs. This differentiation was confirmed by the dendrogram analysis that showed three specific clusters. Four alleles were shared in the WPs2012xBSs. Positive FIS values indicated the presence of endogamy in seven out of ten samples obtained from the WPs. The AMOVA analysis and FST values indicated moderate and high genetic differences in WPsxWPs and high genetic differences in WPsxBSs. The genetic distance and genetic identity values and number of migrants confirmed these results. Adequate intrapopulation genetic variability, similarity between BSsxBSs, and genetic differences between WPs2011xWPs2012 and WPsxBSs were observed. In the wild, individuals from the restocking program were partially observed. ResumoPiaractus mesopotamicus é um peixe tropical que nos últimos anos tem apresentado uma diminuição no número de populações naturais. Programas de repovoamento vêm sendo utilizados como método de conservação, entretanto, o monitoramento genético das populações e dos estoques de reprodutores é importante para conferir a viabilidade desse tipo de programas. O objetivo do presente estudo foi avaliar de forma inédita a diversidade genética de populações selvagens (WPs) e estoques de reprodutores (BSs) de P. mesopotamicus utilizados em programas de repovoamento dos rios Tietê e Grande, através de marcadores microssatélite. Seis loci microssatélite foram amplificados usando DNA extraído de nadadeira caudal de 279 indivíduos adultos. Foi observada alta variabilidade genética intrapopulacional, com medias de heterozigosidade observada entre 0.203 e 0.833. O número de alelos por locus foi de três (locus Pme28 e Pme32) a 13 (locus Pme4, Pme5 e Pme14) e houve diferenciação de alelos entre WPsxWPs e WPsxBSs. Essa diferenciação foi confirmada pela análise do dendrograma que mostrou a formação de três agrupamentos específicos. Observaram-se quatro alelos compartilhados entre WPs2012xBSs. Valores positivos de F IS mostraram a presença de endogamia em sete das 10 coletas realizadas nas WPs. A análise de AMOVA e do F ST indicou moderada e muito alta diferenciação genética entre WPsxWPs e diferenciação gen...

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“…To improve aquaculture production of L. maculatus, a number of studies have been carried out investigating growth, feeding, and population structure in this species (Lee and Yang, 2002;Liu et al, 2006;Li et al, 2012;An et al, 2013An et al, , 2014Wang et al, 2015a,b). Although the selection and rearing of breeders with high-productive properties are important tools to improve progeny quality in fish farming (Borrell et al, 2007), efficient breeding programs are currently lacking for L. maculatus.…”

Section: Introductionmentioning

confidence: 99%

Optimization of selective breeding through analysis of morphological traits in Chinese sea bass (Lateolabrax maculatus)

Wang

Chen

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Determining correlations between certain traits of economic importance constitutes an essential component of selective activities. In this study, our aim was to provide effective indicators for breeding programs of Lateolabrax maculatus, an important aquaculture species in China. We analyzed correlations between 20 morphometric traits and body weight, using correlation and path analyses. The results indicated that the correlations among all 21 traits were highly significant, with the highest correlation coefficient identified between total length and body weight. The path analysis indicated that total length (X 1 ), body width (X 5 ), distance from first dorsal fin origin to anal fin origin (X 10 ), snout length (X 16 ), eye diameter (X 17 ), eye cross (X 18 ), and slanting distance from snout tip to first dorsal fin origin (X 19 ) significantly affected body weight (Y) directly. The following multiple-

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Use of microsatellites and a combinatorial optimization approach in the acquisition of gilthead seabream (Sparus aurata L.) broodstocks for hatcheries

Cited by 27 publications

References 39 publications

Morphological and molecular differentiation of wild and farmed gilthead sea bream Sparus aurata: implications for management

Morphological and molecular differentiation of wild and farmed gilthead sea bream Sparus aurata: implications for management

Genetic diversity of pacu for restocking programs in the Tietê and Grande rivers, Brazil

Optimization of selective breeding through analysis of morphological traits in Chinese sea bass (Lateolabrax maculatus)

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