Use of Microfluidic Technology for Cell Separation

Mohamed, Hisham

doi:10.5772/50382

Cited by 6 publications

(4 citation statements)

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“…Microfluidic systems can integrate different kinds of sorting methods based on the physical parameters of cells, providing a perfect interface for the manipulation of single cells and access forces in a variety of ways and allowing a fully autonomous measurement of physical parameters [58]. Particularly, cell separation techniques have been developed for cell concentration purposes (removal of plasma and increase of the cell concentration, mainly haematocrit increase); plasma enrichment (removal of cells from plasma and cells dilution); blood fractioning (separation of blood into different components); cell sorting (separation of cells by type); and cell removal (specific cell sorting that removes only some specific cells), that can work as cell isolation or removal of pathogenics [59].…”

Section: Microfluidic Cell Separation and Sorting Techniquesmentioning

confidence: 99%

“…Apart from these, there are other methods such as paper-based [60,61] and CD based [62,63] methods to separate mainly the plasma from blood [64]. Active technologies, based on microelectromechanical systems, improve the control of fluids using mobile parts or external mechanical forces, and can be based on dielectrophoresis, magnetophoresis, acoustophoresis and optical tweezers mechanisms [58,64]. Passive technologies for controlling fluids do not include external forces or mobile parts, and their control is promoted by diffusion as a function of the channel geometry [64,65,66,67,68,69,70], or intrinsic hydrodynamic forces, such as punch flow fraction, deterministic lateral displacement, inertial forces and intrinsic physical property of the cells [69,70,71,72,73,74], including sieving, which uses the size of micropores, microweirs, membranes and the gap between micropillars arrays for the separation of cells [26,69,70,71,72,73,74,75,76,77,78].…”

Section: Microfluidic Cell Separation and Sorting Techniquesmentioning

confidence: 99%

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Blood Cells Separation and Sorting Techniques of Passive Microfluidic Devices: From Fabrication to Applications

et al. 2019

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Since the first microfluidic device was developed more than three decades ago, microfluidics is seen as a technology that exhibits unique features to provide a significant change in the way that modern biology is performed. Blood and blood cells are recognized as important biomarkers of many diseases. Taken advantage of microfluidics assets, changes on blood cell physicochemical properties can be used for fast and accurate clinical diagnosis. In this review, an overview of the microfabrication techniques is given, especially for biomedical applications, as well as a synopsis of some design considerations regarding microfluidic devices. The blood cells separation and sorting techniques were also reviewed, highlighting the main achievements and breakthroughs in the last decades.

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Section: Microfluidic Cell Separation and Sorting Techniquesmentioning

confidence: 99%

Section: Microfluidic Cell Separation and Sorting Techniquesmentioning

confidence: 99%

Blood Cells Separation and Sorting Techniques of Passive Microfluidic Devices: From Fabrication to Applications

et al. 2019

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“…To move beyond proof of concept prototypes, these diverse applications of selective cell isolation require high accuracy and reproducibility [ 8 ]. Conventional cell isolation systems such as fluorescence-activated cell sorter (FACS), magnetic activated cell sorting (MACS) and centrifugation systems have demonstrated high robustness, accuracy and throughput and have high utility in industrial and lab settings.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic Platform for Cell Isolation and Manipulation Based on Cell Properties

Yousuff

et al. 2017

Micromachines

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In molecular and cellular biological research, cell isolation and sorting are required for accurate investigation of a specific cell types. By employing unique cell properties to distinguish between cell types, rapid and accurate sorting with high efficiency is possible. Though conventional methods can provide high efficiency sorting using the specific properties of cell, microfluidics systems pave the way to utilize multiple cell properties in a single pass. This improves the selectivity of target cells from multiple cell types with increased purity and recovery rate while maintaining higher throughput comparable to conventional systems. This review covers the breadth of microfluidic platforms for isolation of cellular subtypes based on their intrinsic (e.g., electrical, magnetic, and compressibility) and extrinsic properties (e.g., size, shape, morphology and surface markers). The review concludes by highlighting the advantages and limitations of the reviewed techniques which then suggests future research directions. Addressing these challenges will lead to improved purity, throughput, viability and recovery of cells and be an enabler for novel downstream analysis of cells.

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“…Therefore, all microfluidic cell sorting must furnish a gentle means of cell extraction and handling when performing on naturally live cells (Gross et al, 2015). Nevertheless, the other key challenges in cell sorting not only in all traditional methods but microfluidics are the discrimination of sorted cells in their sizes, densities, shapes, surface protein molecules (Mohamed, 2012), albeit these could be utilized for cell sorting, they are however bringing about a resolution interference of microdevice as well.…”

Section: Microfluidic Platforms For Single Cell Sorting and Entrapmentmentioning

confidence: 99%

Microfluidics-Based Single Cell Isolation and Trapping of Canine Cutaneous Mast Cell Tumor Cells

Ketpun

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In this research, the primary objective was to develop a microfluidic platform utilized for the size-based single cell sorting of canine cutaneous mast cell tumor (MCT) cells which were categorized into 4 groups; I, II, III and IV, upon their individual sizes ranged from 5-9, 10-14, 15-19, and equal to or over than 20 mm respectively. And the secondary aim was to establish the microfluidic-based entrapping microdevice which had a capacity to ensnare single MCT cells into its triangular microwell array at the ratio of one cell per one microwell. The study consequence consistently indicated the accomplishment of device developments. All microfluidic devices were designed and fabricated under the standard soft-lithography. Finally, two microfluidic devices were constructed; the Archimedian spiral microchannel (ASM) and the triangular microwell array. The configuration of ASM consisted of 5-turned, 500mm-wide x 130mm-high, rectangular spiral microchannel with 720mm-long conical exit microchannel separated into 10 asymmetric exit slots. Meanwhile, the entrapment microdevice were composed of the array of 40mm-high x 15mm-deep triangular microwell with 160mm-high main flow channel. The study result indicated that, at the flow rate 1 ml. min-1, the spiral microchannel had a capability to separate single MCT cells in group III and IV, which were the targeted cells, out of the MCT cells group I and II, based upon their sizes. However, the detrimental effects of fluid shear stress and extensional stress in the spiral microchannel, exhibited by the computational simulations, were required an amendment. Because, under hemocytometry quantification, trypan blue and TMRM staining, light-microscopy and SEM morphological assessments and leaky DNA assays, they suggested that the amount of sorted MCT cells was significantly reduced and the cell vitality was only 40 percent. Meanwhile, the morphological assays also confirmed cell elongation and/or wreckage causing the 2.4-fold increment of leaky DNA. For single MCT cell entrapment, the study consequence exhibited that, under pulsatile flow (0. 01 ml. h-1 inflow rate for 30 sec and 3min pausing interval), the trapping microdevice had an ability to stochastically entrap MCT cells in its triangular microwell array. And the efficacy of the microdevice for single and multiple MCT cell entrapment was 35% and 15% respectively. In the epilogue, this study is the first report on the attainments in microfrabication engaged in veterinary field for size-based single MCT cell sorting and entrapment. In addition, there are a tendency to merge these two microdevice components together to form a complete microfluidic-based single cell sorting, trapping and culturing on a chip which is essentially benefit to biomedical engineering researches in the future.

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Use of Microfluidic Technology for Cell Separation

Cited by 6 publications

References 83 publications

Blood Cells Separation and Sorting Techniques of Passive Microfluidic Devices: From Fabrication to Applications

Blood Cells Separation and Sorting Techniques of Passive Microfluidic Devices: From Fabrication to Applications

Microfluidic Platform for Cell Isolation and Manipulation Based on Cell Properties

Microfluidics-Based Single Cell Isolation and Trapping of Canine Cutaneous Mast Cell Tumor Cells

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