Use of metal chelate affinity chromatography and membrane-based ion-exchange as clean-up procedure for trace residue analysis of tetracyclines in animal tissues and egg

Croubels, Siska; Vanoosthuyze, Kristina; Peteghem, Carlos H. Van

doi:10.1016/s0378-4347(96)00368-4

Cited by 61 publications

(35 citation statements)

References 11 publications

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“…Moreover, most chemical methods require a complicated sample preparation step. Commonly, cleanup methods are based on solid-phase extraction (Furusawa, 1999;Cherlet et al, 2003;Cliquet et al, 2003) or metal chelate affinity chromatography (Croubels et al, 1997). These methods are time-consuming and costly, and require large sample volumes.…”

Section: Introductionmentioning

confidence: 99%

Production of a monoclonal antibody against oxytetracycline and its application for oxytetracycline residue detection in shrimp

Wongtangprasert

Natakuathung

Pimpitak

et al. 2014

J. Zhejiang Univ. Sci. B

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A novel monoclonal antibody (MAb) against oxytetracycline (OTC) was generated and characterized. The MAb was used in the development of an enzyme-linked immunosorbant assay (ELISA)-based detection system. An OTC-bovine serum albumin (BSA) conjugate was prepared and used in the immunization of mice. A conventional somatic cell fusion technique was used to generate MAb-secreting hybridomas denoted 2-4F, 7-3G, and 11-11A. An indirect competitive ELISA (icELISA) was applied to measure the sensitivity and specificity of each MAb in terms of its 50% inhibitory concentration (IC 50 ) and percentage of cross-reactivity, respectively. MAb 2-4F exhibited the highest sensitivity, with an IC 50 of 7.01 ng/ml. This MAb showed strong cross-reactivity to rolitetracycline, but no crossreactivity to other unrelated antibiotics. When MAb 2-4F was used to detect OTC from shrimp samples, the recoveries were in the range of 82%-118% for an intra-assay and 96%-113% for an inter-assay. The coefficients of variation of the assays were 3.9%-13.9% and 5.5%-14.9%, respectively.

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Section: Introductionmentioning

confidence: 99%

Production of a monoclonal antibody against oxytetracycline and its application for oxytetracycline residue detection in shrimp

Wongtangprasert

Natakuathung

Pimpitak

et al. 2014

J. Zhejiang Univ. Sci. B

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“…Isolation of TCs from tissues is usually followed by an SPE clean-up procedure using RP materials and metal blocking agents [9, 16 -18, 24, 25, 28, 30], while liquid -liquid extraction is rarely used [16,19,29] as it is time consuming and needs large amounts of organic solvents. Metal chelate affinity chromatography (MCAC) off-and on-line, based on the chelating ability of TCs, as well as exchange extraction membranes, and XAD-2 resins have also been employed as purification methods [13,26,28,31]. Disadvantages of these procedures are mainly focused on their complication and their poor recoveries, so that in most cases an extra SPE step is demanded.…”

Section: Introductionmentioning

confidence: 99%

Development and validation of an HPLC confirma‐tory method for the determination of tetracycline antibiotics residues in bovine muscle according to the European Union regulation 2002/657/EC

Samanidou

Nikolaidou

Papadoyannis³

2005

J of Separation Science

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A high-performance liquid chromatographic method with diode-array detection, at 351 nm, was developed and validated for the determination of five tetracyclines (TCs): minocycline, tetracycline, oxytetracycline, chlortetracycline, and doxycycline in bovine muscle. Samples were macerated with a buffer solution, centrifuged, and purified using Abselut Nexus SPE cartridges. The separation of the examined TCs was achieved on an Inertsil ODS-3 5 microm, 250 x 4 mm analytical column, at ambient temperature. A multistep gradient elution was followed using 0.05 M oxalic acid and CH3CN, at a flow rate of 1.65 mL/min. The procedure was validated according to the European Union regulation 2002/657/EC determining selectivity, stability, decision limit, detection capability, accuracy, and precision. The results of the validation process demonstrate that the method can be readily applied to European Union statutory veterinary drug residue surveillance programmes. Mean recoveries of TCs from bovine muscle samples spiked at three concentrations (100, 250, and 400 ng/g) were in the range of 98.7-103.3%. Method's LOQ values achieved were 40 microg/kg for MNC, CTC, and DC and 25 microg/kg for OTC and TC. The decision limits (CCalpha) were in the range of 104.7-109.8 microg/kg, while the detection capability (CCbeta) was in the range of 108.4-116.7 microg/kg for all compounds.

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“…In different studies the use of polymeric (8,14,15,29), phenyl (23), C8-bonded silica (2), or C18 analytical columns to analyse TCs in animal tissues (4, 7) have been presented. Polymeric columns were chosen to eliminate interaction of silanols and trace metals, but Mc Cracken et al (22) obtained less satisfactory results with a polymer column versus a commonly use C8 or C18 analytical column.…”

Section: Discussionmentioning

confidence: 99%

“…As described above, TCs have a chelating ability, which has been used to establish a clean-up method, such as metal chelate affinity chromatography (12,14). However, this method requires complicated procedures and gives poor recovery and reproducibility.…”

Section: Discussionmentioning

confidence: 99%

Liquid chromatography – tandem mass spectrometry method for the determination of ten tetracycline residues in muscle samples

Gajda

Posyniak

2015

Bulletin of the Veterinary Institute in Pulawy

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A liquid chromatography -tandem mass spectrometry (LC-MS/MS) method for the determination of oxytetracycline (OTC), 4-epi oxytetracycline (4-epi OTC), tetracycline (TC), 4-epi tetracycline (4-epi TC), chlortetracycline (CTC), 4-epi chlortetracycline (4-epi CTC), doxycycline (DC), minocycline (MINO), methacycline (META) and rolitetracycline (ROLI) residues in muscles was developed. The procedure consisted of an oxalic acid extraction followed by protein removal with trichloroacetic acid. Further solid phase clean-up on polymeric (Strata X) reversed phase columns was performed to obtain an extract suitable for LC-MS/MS analysis. The tetracyclines were separated on a C 18 analytical column with mobile phase consisting of 0.01% formic acid in acetonitrile and 0.01% formic acid in water in gradient mode. The method was validated according to the Commission Decision 2002/657/EC. The recoveries of all target compounds were 91.8% -103.6%. The decision limits were from 109.0 to 119.8 µg/kg and detection capability varied within the range of 122.2 to 137.6 µg/kg, depending on the analyte.

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Use of metal chelate affinity chromatography and membrane-based ion-exchange as clean-up procedure for trace residue analysis of tetracyclines in animal tissues and egg

Cited by 61 publications

References 11 publications

Production of a monoclonal antibody against oxytetracycline and its application for oxytetracycline residue detection in shrimp

Production of a monoclonal antibody against oxytetracycline and its application for oxytetracycline residue detection in shrimp

Development and validation of an HPLC confirma‐tory method for the determination of tetracycline antibiotics residues in bovine muscle according to the European Union regulation 2002/657/EC

Liquid chromatography – tandem mass spectrometry method for the determination of ten tetracycline residues in muscle samples

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