Use of Membrane Filter Technique in the Microbiological Control for the Brewing Industry

Abstract

A physical method was developed involving serial filtration with membrane filters for separating yeast cells from bacteria. Such a method eliminates the need for antibiotics previously required to permit differential counting of such populations. All yeast cells filtered were successfully retained and cultivated on a 1.2-,u membrane filter by use of a synthetic medium. All bacteria filtered avoided entrapment on a

Comparative Poliovirus Permeability of Silver, Polycarbonate, and Cellulose Membrane Filters

“…The polycarbonate filter poliovirus is presently available only in a relatively large pore d polycar-size, which limits its utility to fungi and larger )osition of bacteria. The availability of smaller pore sizes in ations, no either polycarbonate or silver filters would aid with cellu-greatly in the sizing of different viruses, in test m the me-methods for the certification of sterile heat-labile d virtually ifitrates as described by Portner et al (10), and rus type 1 in microbiological control methods in the brewing b strain of industry (9).…”

High-Throughput Rapid and Inexpensive Assay for Quantitative Determination of Low Cell-Density Yeast Cultures

“…When cell counting is applied to cells on surfaces, such as microbial biofilms [15,16], the problems are made more complex by the necessity of detaching the cells from the surfaces, implying that some counting could be affected by underestimation, due to the low efficacy of the detaching treatment [17]. These methods can be applied for research in a microbiology laboratory, but most would be inappropriate for applicative environments, where high throughput and a swift procedure are as essential as low cost per operation [18,19,20]. This can be the case of monitoring [21] for the presence of cells, even at low densities, in biotechnological industries.…”