Use of magnetic resonance imaging (MRI) and micro-computed tomography (micro-CT) in the morphological examination of rat and rabbit fetuses from embryo-fetal development studies

French, Julian; Gingles, Neill A.; Stewart, Jane; Woodhouse, Neil

doi:10.1016/j.reprotox.2010.04.016

Cited by 21 publications

(7 citation statements)

References 10 publications

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“…Thayyil et al . were the first to propose the study of small fetuses with micro‐MRI, a well‐established method for examining small laboratory animals. In the 17 fetuses (age, 11–22 weeks; weight, 5–400 g) that they studied with a 9.4‐T micro‐MRI scanner, the image resolution used (200 µm) was reportedly sufficient for detecting the structural abnormalities that could be identified with conventional autopsy and also for internal examination of the visceral organs.…”

Section: Discussionmentioning

confidence: 99%

Postmortem microcomputed tomography (micro‐CT) of small fetuses and hearts

Lombardi

Zambelli

Botta

et al. 2014

Ultrasound in Obstet & Gyne

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Section: Discussionmentioning

confidence: 99%

Postmortem microcomputed tomography (micro‐CT) of small fetuses and hearts

Lombardi

Zambelli

Botta

et al. 2014

Ultrasound in Obstet & Gyne

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“…The MRI system detects changes in the magnetic field of hydrogen atoms within the sample, converting this information into 2D or 3D images of the sample. For small animal imaging, MRI systems with high-magnetic field strengths (micro-MRI) with 7 to 14-Tesla magnets are commercially available (Schneider et al, 2004;Yelbuz et al, 2004;Cleary et al, 2009;Pallares et al, 2009;Parnell et al, 2009;French et al, 2010;Yamada et al, 2010;Zouagui et al, 2010). These systems have imaging resolution of 20 lm, similar to micro-CT and better than ultrasound biomicroscopy.…”

Section: Micromagnetic Resonance Imagingmentioning

confidence: 99%

Imaging modalities to assess structural birth defects in mutant mouse models

Tobita

Liu

2010

Birth Defects Research Pt C

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Assessment of structural birth defects (SBDs) in animal models usually entails conducting detailed necropsy for anatomical defects followed by histological analysis for tissue defects. Recent advances in new imaging technologies have provided the means for rapid phenotyping of SBDs, such as using ultra-high frequency ultrasound biomicroscopy, optical coherence tomography, micro-CT, and micro-MRI. These imaging modalities allow the detailed assessment of organ/tissue structure, and with ultrasound biomicroscopy, structure and function of the cardiovascular system also can be assessed noninvasively, allowing the longitudinal tracking of the fetus in utero. In this review, we briefly discuss the application of these state-of-the-art imaging technologies for phenotyping of SBDs in rodent embryos and fetuses, showing how these imaging modalities may be used for the detection of a wide variety of SBDs.

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“…Furthermore, tiny structures like the cardiac valves cannot be visualized in detail using micro-CT without the use of high-resolution systems that can add substantially to image acquisition and image processing times. Another noninvasive technology that has been used frequently for imaging animal models is micro-MRI (Yelbuz et al, 2004;Petiet et al, 2008;Berrios-Otero et al, 2009;Cleary et al, 2009;Hogers et al, 2009;French et al, 2010;Nieman and Turnbull, 2010;Tobita et al, 2010;Zouagui et al, 2010). This method can provide relatively high resolution images (20-25 mm) without using contrast agents or harmful radiation like micro-CT.…”

Section: Introductionmentioning

confidence: 99%

Using optical coherence tomography to rapidly phenotype and quantify congenital heart defects associated with prenatal alcohol exposure

Karunamuni

Doughman

et al. 2015

Developmental Dynamics

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Background The most commonly used method to analyze congenital heart defects involves serial sectioning and histology. However, this is often a time-consuming process where the quantification of cardiac defects can be difficult due to problems with accurate section registration. Here we demonstrate the advantages of using optical coherence tomography, a comparatively new and rising technology, to phenotype avian embryo hearts in a model of Fetal Alcohol Syndrome where a binge-like quantity of alcohol/ethanol was introduced at gastrulation. Results The rapid, consistent imaging protocols allowed for the immediate identification of cardiac anomalies, including ventricular septal defects and misaligned/missing vessels. Interventricular septum thicknesses and vessel diameters for three of the five outflow arteries were also significantly reduced. Outflow and atrio-ventricular valves were segmented using image processing software and had significantly reduced volumes compared to controls. This is the first study to our knowledge that has 3-D reconstructed the late-stage cardiac valves in precise detail in order to examine their morphology and dimensions. Conclusion We believe therefore that OCT, with its ability to rapidly image and quantify tiny embryonic structures in high resolution, will serve as an excellent and cost-effective preliminary screening tool for developmental biologists working with a variety of experimental/disease models.

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Use of magnetic resonance imaging (MRI) and micro-computed tomography (micro-CT) in the morphological examination of rat and rabbit fetuses from embryo-fetal development studies

Cited by 21 publications

References 10 publications

Postmortem microcomputed tomography (micro‐CT) of small fetuses and hearts

Postmortem microcomputed tomography (micro‐CT) of small fetuses and hearts

Imaging modalities to assess structural birth defects in mutant mouse models

Using optical coherence tomography to rapidly phenotype and quantify congenital heart defects associated with prenatal alcohol exposure

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