Use of low volatility mobile phases in electroosmotic thin-layer chromatography

Berezkin, V. G.; Balushkin, A. O.; Tyaglov, B. V.; Litvin, E. F.

doi:10.1016/j.chroma.2004.12.093

Cited by 12 publications

(5 citation statements)

References 8 publications

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“…These results are both theoretically and practically important because they indicate how medical diagnostic test strips may be fabricated without incorporating an absorbent pad, the standard means of generating sustained liquid flow in lateral flow assays sold commercially today. Finally, the approach described here for generating quasi-stationary flow is potentially applicable to thin layer chromatography, particularly recent embodiments which require continuous flow. − ,− …”

Section: Discussionmentioning

confidence: 99%

“…The advent of medical lateral flow assays in the 1950s capped a series of developments in paper-based spot tests and chromatographic separations dating back to the 1830s. , In recent years, interest in the medical utility of paper substrates has resulted in the development of two-dimensional lateral flow assays , and three-dimensional microfluidics . The closely related technique of thin layer chromatography continues to receive significant attention, − as do experimental and theoretical studies of imbibition. − …”

Section: Introductionmentioning

confidence: 99%

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Imbibition in Porous Membranes of Complex Shape: Quasi-stationary Flow in Thin Rectangular Segments

et al. 2009

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The sustained liquid flow of a typical lateral flow assay can be mimicked by two-dimensional shaped, thin porous membranes, specifically rectangular membranes appended to circular sectors. In designing these fan-shaped devices, we have been aided by analytical equations and finite-element simulations. We show both mathematically and experimentally how a continuous increase in unwetted pore volume causes a deviation from traditional imbibition, and leads to quasi-stationary flow in the rectangular element. These results are both theoretically and practically important because they indicate how medical diagnostic test strips may be fabricated without incorporating an absorbent pad.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Imbibition in Porous Membranes of Complex Shape: Quasi-stationary Flow in Thin Rectangular Segments

et al. 2009

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“…Substantial progresses in chemical analysis have been achieved with on-paper separation methods through sophisticated derivatization of the paper substrates. [6–8] Combinations of the on-paper separation with other analytical techniques have also been wildly used, among which the mass spectrometry (MS) is a major method for the qualitative and quantitative analysis after the chemical separation [7, 9–11]. …”

Section: Introductionmentioning

confidence: 99%

Analysis of Biological Samples Using Paper Spray Mass Spectrometry: An Investigation of Impacts by the Substrates, Solvents and Elution Methods

Ren

Liu

et al. 2013

Chromatographia

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Paper spray has been developed as a fast sampling ionization method for direct analysis of raw biological and chemical samples using mass spectrometry (MS). Quantitation of therapeutic drugs in blood samples at high accuracy has also been achieved using paper spray MS without traditional sample preparation or chromatographic separation. The paper spray ionization is a process integrated with a fast extraction of the analyte from the raw sample by a solvent, the transport of the extracted analytes on the paper, and a spray ionization at the tip of the paper substrate with a high voltage applied. In this study, the influence on the analytical performance by the solvent-substrate systems and the selection of the elution methods was investigated. The protein hemoglobin could be observed from fresh blood samples on silanized paper or from dried blood spots on silica-coated paper. The on-paper separation of the chemicals during the paper spray was characterized through the analysis of a mixture of the methyl violet 2B and methylene blue. The mode of applying the spray solvent was found to have a significant impact on the separation. The results in this study led to a better understanding of the analyte elution, on-paper separation, as well as the ionization processes of the paper spray. This study also help to establish a guideline for optimizing the analytical performance of paper spray for direct analysis of target analytes using mass spectrometry.

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“…Pressurized planar electrochromatography (PPEC) eliminates these limitations and, in addition, affords faster separations with enhanced efficiency . Pressurization also provides a linear voltage drop and constant EOF. , Developments in planar electrochromatography have been summarized in recent excellent reviews. − …”

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confidence: 99%

“…planar electrochromatography have been summarized in recent excellent reviews. [18][19][20] In this letter we present our initial results demonstrating the use of monolithic thin layers prepared from an organic polymer in the rapid separation of peptides. This subject is getting significant impetus by the current interest in proteomics.…”

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confidence: 99%

Separation of Peptides and Oligonucleotides Using a Monolithic Polymer Layer and Pressurized Planar Electrophoresis and Electrochromatography

et al. 2010

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The rapid separation of mixtures of six peptides using porous polymer monolithic layers in electrophoresis and pressurized planar electrochromatography modes has been achieved. The separations in the former mode were performed on a generic hydrophobic poly(butyl methacrylate-co-ethylene dimethacrylate) layer with no ionizable functionalities and required 2 min. This layer also enabled the separation of three oligonucleotides. The separation in the pressurized planar electrochromatographic mode was carried out using a negatively charged layer prepared via cografting of 2-acrylamido-2-methyl-1-propanesulfonic acid and 2-hydroxyethyl methacrylate on top of the generic hydrophobic monolith and was completed in 1 min.

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Use of low volatility mobile phases in electroosmotic thin-layer chromatography

Cited by 12 publications

References 8 publications

Imbibition in Porous Membranes of Complex Shape: Quasi-stationary Flow in Thin Rectangular Segments

Imbibition in Porous Membranes of Complex Shape: Quasi-stationary Flow in Thin Rectangular Segments

Analysis of Biological Samples Using Paper Spray Mass Spectrometry: An Investigation of Impacts by the Substrates, Solvents and Elution Methods

Separation of Peptides and Oligonucleotides Using a Monolithic Polymer Layer and Pressurized Planar Electrophoresis and Electrochromatography

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