Use of low-cost substrates for cost-effective production of extracellular and cell-bound lipases by a newly isolated yeast Dipodascus capitatus A4C

Baloch, Khurshid Ahmed; Upaichit, Apichat; Cheirsilp, Benjamas

doi:10.1016/j.bcab.2019.101102

Cited by 11 publications

(4 citation statements)

References 34 publications

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“…It was found that S. hominis AUP19 exhibited higher lipase activity in the BSM medium than in the enrichment medium (EBSM) due to the presence of glucose in BSM. In the lipase production by Dipodascus capitatus A4C in a lipase production medium, enriched with 2% (w/v) glucose, resulting in maximum ECL synthesis of 2513 U/L after 72 h and CBL with hydrolytic activity up to 620 U/L after 24 h [67]. Accordingly, from the above results, POME100 at pH 7.0 was suitable for CBL production.…”

Section: Selection Of the Cbl-producing Strainsmentioning

confidence: 75%

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Low-cost production of cell-bound lipases by pure and co-culture of yeast and bacteria in palm oil mill effluent and the applications in bioremediation and biodiesel synthesis

Fibriana

Upaichit

Cheirsilp

2021

Biomass Conv. Bioref.

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Two bacterial and six yeast strains capable of lipase production were investigated for their extracellular lipase (ECL), cellbound lipase (CBL), and growth profile in basal standard medium (BSM) and the low-cost palm oil mill effluent (POME) media. The yeast Magnusiomyces spicifer AW2 and bacteria Staphylococcus hominis AUP19 were selected and further employed to explore their survivability, lipase activity, and cell biomass production in various POME concentrations. It was revealed that diluted POME 1:1 at pH 7.0 was suitable for yeast and bacterial performance. Furthermore, the development of co-culture between yeast and bacteria was performed by mixing them at a ratio of 1:1. The synthetic co-culture system was successful in improving CBL activity to 3860 U/L. The bioremediation parameter, i.e., oil and grease (O&G) removal, was efficiently achieved 1.1-fold higher than yeast alone and bacterial monoculture at 80.1 ± 1.3%. At the same time, COD removal was highest with the co-culture treatment of 75.9 ± 2.8%, indicating 1.3-fold more effective than yeast monoculture and 1.4-fold better than single bacterial form. The mixed cells of two strains effectively improved biodiesel production from oleic acid of 73.5 ± 3.3% and palm oil of 82.5 ± 0.3% under solvent-free conditions for 72-h reactions at room temperature (30 ± 2 °C), indicating a promising prospect of a combined approach of POME treatment and valorization.

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Section: Selection Of the Cbl-producing Strainsmentioning

confidence: 75%

“…One-unit enzyme activity is defined as the enzyme necessary to release 1 µmol of palmitic acid per minute at the specified conditions. ECL activity was measured based on the volume of the enzyme (U/L), whereas CBL activity was measured based on hydrolytic activity and dry cell weight (U/L) [23,60,67].…”

Section: Lipase Activity Assaysmentioning

confidence: 99%

Low-cost production of cell-bound lipases by pure and co-culture of yeast and bacteria in palm oil mill effluent and the applications in bioremediation and biodiesel synthesis

Fibriana

Upaichit

Cheirsilp

2021

Biomass Conv. Bioref.

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“…The potential of molasses as a sole carbon source has been evaluated with various microorganisms. Beet molasses was used in growth medium of Yarrowia lipolytica for recombinant laccase [ 126 ] and Dipodascus capitatus A4C for recombinant lipase production [ 127 ]. Zhoukun et al [ 108 ] presented that recombinant α-amylase from E. coli could be produced efficiently in P. pastoris with an industrial waste of molasses.…”

Section: Introductionmentioning

confidence: 99%

Second generation Pichia pastoris strain and bioprocess designs

Ergün

Laçın

Çaloğlu

et al. 2022

Biotechnol Biofuels

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Yeast was the first microorganism used by mankind for biotransformation processes that laid the foundations of industrial biotechnology. In the last decade, Pichia pastoris has become the leading eukaryotic host organism for bioproduct generation. Most of the P. pastoris bioprocess operations has been relying on toxic methanol and glucose feed. In the actual bioeconomy era, for sustainable value-added bioproduct generation, non-conventional yeast P. pastoris bioprocess operations should be extended to low-cost and renewable substrates for large volume bio-based commodity productions. In this review, we evaluated the potential of P. pastoris for the establishment of circular bioeconomy due to its potential to generate industrially relevant bioproducts from renewable sources and waste streams in a cost-effective and environmentally friendly manner. Furthermore, we discussed challenges with the second generation P. pastoris platforms and propose novel insights for future perspectives. In this regard, potential of low cost substrate candidates, i.e., lignocellulosic biomass components, cereal by-products, sugar industry by-products molasses and sugarcane bagasse, high fructose syrup by-products, biodiesel industry by-product crude glycerol, kitchen waste and other agri-food industry by products were evaluated for P. pastoris cell growth promoting effects and recombinant protein production. Further metabolic pathway engineering of P. pastoris to construct renewable and low cost substrate utilization pathways was discussed. Although, second generation P. pastoris bioprocess operations for valorisation of wastes and by-products still in its infancy, rapidly emerging synthetic biology tools and metabolic engineering of P. pastoris will pave the way for more sustainable environment and bioeconomy. From environmental point of view, second generation bioprocess development is also important for waste recycling otherwise disposal of carbon-rich effluents creates environmental concerns. P. pastoris high tolerance to toxic contaminants found in lignocellulosic biomass hydrolysate and industrial waste effluent crude glycerol provides the yeast with advantages to extend its applications toward second generation P. pastoris strain design and bioprocess engineering, in the years to come. Graphical Abstract

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“…Microorganisms of the genus Aspergillus, Calvatia, Rhizopus, Rhodotorula and Yarrowia can produce constitutive lipase (gene expression) that can be partially induced with the aid of triacylglycerols or fatty acids. The physiological role of lipases for yeast cells is, basically, to degrade oils and fatty acids and, therefore, contribute to the increase of cell mass (Baloch et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Catalytic and physical features of a naturally immobilized Yarrowia lipolytica lipase in cell debris (LipImDebri) displaying high thermostability

et al. 2020

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Lipase activity (337 U/g dry weight of cell debris) was detected in cell debris after ultrasound treatment of Yarrowia lipolytica cells cultivated in residual frying palm oil. It is a naturally immobilized lipase with protein content of 47%, herein called LipImDebri. This immobilized biocatalyst presents low hydrophobicity (8%), that can be increased adjusting pH and buffer type. Despite apparent intact cells, electron microscopy showed a shapeless and flat surface for LipImDebri and optical microscopy revealed no cell viability. Besides, an inferior mean diameter (3.4 mm) in relation to whole cells reveals structure modification. A high negative zeta potential value (− 33.86 mV) for pH 6 and 25 °C suggests that LipImDebri is a stable suspension in aqueous solution. Fourier Transform Infrared Spectra (FTIR) expose differences between LipImDebri and extracellular lipase extract signaling a physical interaction between enzyme and cell debris, which is possibly the reason for the high thermostability (k d = 0.246 h −1 ; t 1/2 = 2.82 h at 50 °C, pH 7.0). A good adjustment of LipImDebri kinetic data with Hill equation (R 2 = 0.95) exposes an allosteric behavior related to the presence of more than one lipase isoform. These features reveal that LipImDebri can be a good catalyst for industrial applications.

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Use of low-cost substrates for cost-effective production of extracellular and cell-bound lipases by a newly isolated yeast Dipodascus capitatus A4C

Cited by 11 publications

References 34 publications

Low-cost production of cell-bound lipases by pure and co-culture of yeast and bacteria in palm oil mill effluent and the applications in bioremediation and biodiesel synthesis

Low-cost production of cell-bound lipases by pure and co-culture of yeast and bacteria in palm oil mill effluent and the applications in bioremediation and biodiesel synthesis

Second generation Pichia pastoris strain and bioprocess designs

Catalytic and physical features of a naturally immobilized Yarrowia lipolytica lipase in cell debris (LipImDebri) displaying high thermostability

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