Use of Liquid Chromatography with Electrochemical Detection for the Determination of Antioxidants in Less Common Fruits

Abstract

Neurodegenerative disorders (NDD) have become the common global health burden over the last several decades. According to World Health Organization (WHO), a staggering 30 million people will be affected by Alzheimer’s disease in Europe and the USA by 2050. Effective therapies in this complex field considering the multitude of symptoms associated with NDD indications, have not been found yet. Based on the results of NDD related studies, prevention appears to be the promise alternative. Antioxidative and anti-inflammatory properties are hypothesized for natural phenolics, a group of plant secondary products that may positively impact neurodegenerative diseases. In these studies, phenolic-rich extracts from less common fruit species: Blue honeysuckle (Lonicera edulis, Turcz. ex. Freyn), Saskatoon berry (Amelanchier alnifolia Nutt.), and Chinese hawthorn (Crateagus pinnatifida Bunge) were obtained and analyzed to detect neuroprotective substances content and establish a potential therapeutic value. High performance liquid chromatography with electrochemical detection was optimized and further applied on analysis of the extracts of less common fruit species. It was observed that Chinese hawthorn and Blue honeysuckle extracts are potent source of neuroprotective phenolic antioxidants. In accordance the results, it appears that the fruit or formulated products may have the potential for the prevention of neurodegenerative diseases.

Antocianinų kiekis sausmedžio (Lonicera kamtschatica) klonuose šaldymo metu, priklausomai nuo antioksidantinės ir maistinės vertės rodiklių

“…Among flavonoids proanthocyanins, anthocyanins, quercetin, isoquercetin and rutin are predominant. At the same time, anthocyanins represented the group of flavonoids which mostly contributed to antioxidant activity of honeyberry fruit as this has been confirmed by the studies of Chaovanalikit et al (2004) and Gazdik et al (2008).…”

“…According to Gazdik et al (2008), Jurikova and Matuskovic (2007), Jurikova et al (2012) and Rop et al (2011), the antioxidant activity of honeyberry fruit was high due to high level of the total flavonoid and total polyphenol content. On the other hand, in our experiment the statistically significant weak negative correlation between anthocyanin content and TAC of fruit (R = −0.585) at α = 0.05 was proved.…”

“…cancer (Gruia et al, 2008), neurodegenerative diseases (Gazdik et al, 2008), their anti-inflammatory effect has been proved as well (Svobodova et al, 2009). The colour stability of anthocyanin depends on the combination of various factors such as the structure and concentration of anthocyanin, pH, temperature and presence of complexing agents (phenols, metals) or other chemical substances (Jurikova et al, 2012).…”

“…One of the possibilities how to express the biological value of foodstuff is the determination of antioxidant capacity, concurrently with the assessment of mainly presented bioactive components in berries (Gazdik et al, 2008). The honeyberries represent a lesser-known berry crop originated from the territory of Russia, rich in antioxidants; with a high content of ascorbic acid and polyphenolic compounds -phenolic acids and flavonoids.…”

Antioxidant and radical scavenging activities in fruits of 6 sea buckthorn (Hippophae rhamnoides L.) cultivars

“…Fruits are among the basic foodstuffs with antioxidant properties (Celik et al, 2009;Ochmian et al, 2009;Poledica et al, 2012;Rab and Lisan-Ul-Haq, 2012). These properties are caused by many chemical compounds, e.g., phenolics or flavonoids (Gazdík et al, 2008a(Gazdík et al, , 2008bLugasi et al, 2011).…”