Use of liposome-immunopotentiated exopolysaccharide as a component of an ovine mastitis staphylococcal vaccine

Amorena, B.; Baselga, R.; Albizu, Iñaki

doi:10.1016/0264-410x(94)90201-1

Cited by 34 publications

(18 citation statements)

References 37 publications

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“…Contudo, devido os diferentes relatos sobre a efetividade destas vacinas para vacas leiteiras e ovelhas, e sua inabilidade para prevenir novas infecções, tem sido sugerido que as vacinas sejam usadas em rebanhos leiteiros com alta prevalên-cia de S. aureus para reduzir os sinais clínicos (Contreras et al 2007). A efetividade dos programas de vacinação contra mastite causada por S. aureus tem sido relatada para ovelhas, mas não para cabras (Amorena et al 1994, Tollersrud et al 2002. Atualmente, estudos sobre a vacinação têm fracassado em ratificar esta técnica como uma ferramenta para o controle da mastite em pequenos ruminantes, sendo estudos sobre imunização, particularmente envolvendo a caracterização dos mecanismos imunológicos de resposta ainda necessários para aprimorar esta estraté-gia (Contreras et al 2007).…”

Section: Estratégias De Controle E Prevençãounclassified

Mastite em pequenos ruminantes no Brasil

2010

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Este artigo objetivou revisar as informações recentes sobre mastite em pequenos ruminantes, abrangendo etiologia, epidemiologia, aspectos de controle e profilaxia. Houve a preocupação em reunir resultados de estudos desenvolvidos no Brasil, uma vez que a mastite tem a interferência de uma série de fatores, como fatores ambientais e outros decorrentes dos sistemas de manejo empregados, condições essas determinantes para etiologia e epidemiologia da enfermidade. A prevalência da mastite em caprinos varia entre 22 e 75%, sendo que os casos de mastite subclínica são os mais frequentes. Existe uma carência de trabalhos voltados para os aspectos epidemiológicos da enfermidade no nosso país. Contudo, observa-se que a mastite vem assumindo importância cada vez maior nos rebanhos voltados para produção de carne, sendo encontrados resultados de pesquisa, principalmente na espécie ovina. A mastite estafilocócica corresponde à maior fração nas infecções intramamárias em pequenos ruminantes. O caráter zoonótico de alguns patógenos, a exemplo do Staphylococcus aureus ressalta a importância da implantação de programas de controle em propriedades leiteiras. Algumas das ferramentas de diagnóstico ainda necessitam de padronização, principalmente para espécie caprina que apresenta uma série de particularidades. Ainda são discutidas as principais estratégias de controle como o manejo de fêmeas e suas crias, os procedimentos de ordenha e a utilização de vacinas.

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Section: Estratégias De Controle E Prevençãounclassified

Mastite em pequenos ruminantes no Brasil

2010

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“…Different vaccination strategies involving surface polysaccharides have been evaluated for efficacy against staphylococcal mastitis in ruminants, encompassing inactivated bacteria and toxoids [11]; bacteria surrounded by a mucous substance (likely a biofilm matrix) termed a pseudo-capsule [12] or slime [13]; capsular polysaccharide (CP), types CP5, CP8 and CP336 linked to protein carriers [14,15,16]; CP5 co-entrapped in liposomes with alpha-toxin [17]; and a mixture of slime in liposomes, toxoid and different inactivated bacteria [18]. Several field trials with these and other vaccines including bacterin-toxoid [19,20,21], crude extracts of encapsulated bacteria in aluminum hydroxide [22,23], have revealed a significant degree of protection against mastitis.…”

Section: Introductionmentioning

confidence: 99%

Protection from Staphylococcus aureus mastitis associated with poly-N-acetyl β-1,6 glucosamine specific antibody production using biofilm-embedded bacteria

Pérez

Prenafeta²,

Valle

et al. 2009

Vaccine

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Staphylococcus aureus vaccines based on bacterins surrounded by slime, surface polysaccharides coupled to protein carriers and polysaccharides embedded in liposomes administered together with non-biofilm bacterins confer protection against mastitis. However, it remains unknown whether protective antibodies are directed to slime-associated known exopolysaccharides and could be produced in the absence of bacterin immunizations. Here, a sheep mastitis vaccination study was carried out using bacterins, crude bacterial extracts or a purified exopolysaccharide from biofilm © 2009 Elsevier Ltd. All rights reserved.Corresponding author: Beatriz Amorena, e Instituto de Agrobiotecnología, CSIC-Universidad Pública de Navarra-Gobierno de Navarra, 31192 Mutilva Baja, Spain. Phone: +34 948 16 80 06; Fax: + 34 948 23 21 91; bamorena@unavarra.es. Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Disclosed potential conflicts of interestDrs. T. Maira-Litran and G.B. Pier declare they have a financial interest in the form of licensing and consulting income for the commercial development of PNAG-based vaccines and immunotherapies related to issued U.S. patents 7,252,828, 7,015,007, 6,743,431, 6,399,066, 5,980,910, and NIH-PA Author ManuscriptNIH-PA Author Manuscript NIH-PA Author Manuscript bacteria delivered in different vehicles. This polysaccharide reacted specifically with antibodies to poly-N-acetyl-β-1,6-glucosamine (PNAG) and not with antibodies to other capsular antigens or bacterial components. Following intra-mammary challenge with biofilm-producing bacteria, antibody production against the polysaccharide, milk bacterial counts and mastitis lesions were determined. Bacterins from strong biofilm-producing bacteria triggered the highest production of antibodies to PNAG and conferred the highest protection against infection and mastitis, compared with weak biofilm-producing bacteria and non-cellular inocula. Thus, bacterins from strong biofilm bacteria, rather than purified polysaccharide, are proposed as a cost-efficient vaccination against S. aureus ruminant mastitis.

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“…In this context and using highly adherent strains, we have observed that S. aureus in vitro adherence and biofilm formation on inert or mammalian cell surfaces is associated with (i) exopolysaccharide production (6,23); (ii) rough colony morphology phenotype in Congo red agar (CRA) (6); (iii) higher resistance to phagocytosis (32); (iv) lower susceptibility to antibiotics when forming biofilms (2); (v) higher capacity to attach to different surfaces and biomaterials used in orthopedic surgery, causing osteomyelitis (16); and (vi) higher capacity to colonize the ovine mammary gland, causing mastitis (6). In addition, active immunizations with exopolysaccharides extracted from a highly adherent S. aureus isolate have been shown to trigger protective immunity against mastitis (3). However, the genetic mechanisms underlying these observations have not been determined.…”

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confidence: 99%

Bap, a Staphylococcus aureus Surface Protein Involved in Biofilm Formation

et al. 2001

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Identification of new genes involved in biofilm formation is needed to understand the molecular basis of strain variation and the pathogenic mechanisms implicated in chronic staphylococcal infections. A biofilmproducing Staphylococcus aureus isolate was used to generate biofilm-negative transposon (Tn917) insertion mutants. Two mutants were found with a significant decrease in attachment to inert surfaces (early adherence), intercellular adhesion, and biofilm formation. The transposon was inserted at the same locus in both mutants. This locus (bap [for biofilm associated protein]) encodes a novel cell wall associated protein of 2,276 amino acids (Bap), which shows global organizational similarities to surface proteins of gram-negative (Pseudomonas aeruginosa and Salmonella enterica serovar Typhi) and gram-positive (Enteroccocus faecalis) microorganisms. Bap's core region represents 52% of the protein and consists of 13 successive nearly identical repeats, each containing 86 amino acids. bap was present in a small fraction of bovine mastitis isolates (5% of the 350 S. aureus isolates tested), but it was absent from the 75 clinical human S. aureus isolates analyzed. All staphylococcal isolates harboring bap were highly adherent and strong biofilm producers. In a mouse infection model bap was involved in pathogenesis, causing a persistent infection.

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Use of liposome-immunopotentiated exopolysaccharide as a component of an ovine mastitis staphylococcal vaccine

Cited by 34 publications

References 37 publications

Mastite em pequenos ruminantes no Brasil

Mastite em pequenos ruminantes no Brasil

Protection from Staphylococcus aureus mastitis associated with poly-N-acetyl β-1,6 glucosamine specific antibody production using biofilm-embedded bacteria

Bap, a Staphylococcus aureus Surface Protein Involved in Biofilm Formation

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