Use of lipase immobilized on celluse support for cleaning aged oil layers

Abstract

O presente estudo relata (i) a imobilização covalente da lipase de Candida rugosa sobre diferentes suportes de celulose (cotonetes, algodão para remoção de maquiagem, pó de celulose, algodão e lenços), usando periodato de sódio como agente ativo, e (ii) suas aplicações na remoção de óleo de linhaça envelhecido, de telas. A otimização das condições experimentais como pH, temperatura e tempo de reação foi realizada para o procedimento de imobilização e para a aplicação do biocatalisador. Assim, as condições ótimas de imobilização foram pH 7,0, 20 o C, 0,3 mg de lipase por mg do suporte e tempo de reação de 200 min, enquanto as condições necessárias para o tratamento da superfície de telas contendo óleo de linhaça envelhecido, foram pH 6, 40o C e tempo de reação de 45 min. A habilidade da lipase imobilizada em remover filmes de óleo envelhecido foi confirmada por análises de espectroscopia UV-Vis, cromatografia líquida de alta eficiência (HPLC) e microscopia eletrônica de varredura (SEM).The present study reports (i) the covalent immobilization of lipase on different cellulose supports (cotton buds, make-up remover pads, cellulose powder, cotton and tissues) using sodium periodate as activating agent and (ii) its application on the aged linseed oil removal from canvas. The optimization of experimental conditions such as pH, temperature and reaction time was performed for both, immobilization procedure and the biocatalyst application. Thus optimal conditions of immobilization were pH 7.0, 20 °C, 0.3 mg lipase loading per mg support and 200 min reaction time, while those for treating canvas surface, having stratified aged linseed oil were pH 6, 40 °C and 45 min reaction time. The ability of the immobilized lipase to remove aged oil films was confirmed by UV-Vis spectroscopy, high performance liquid chromatography (HPLC) and scanning electron microscopic (SEM) analysis.

Enzyme immobilization on cellulose matrixes

“…59 Lipase was immobilized on different cellulose materials, such as cellulose powder, cotton buds, and cotton tissue. 22 Sodium periodate-oxidized pulp fiber was used as a new carrier for pectinase immobilization. 60 Galactose oxidase was also immobilized on magnetic cellulose bead through periodate oxidation.…”

Biocatalyst of Nanocomposite using Functionalized Low-Cost Activated Carbon from Zea mays L. (maize) Husk Leaf with Lipase for Hydrolysis of Olive Oil

“…One unit of lipase activity (U) is defined as the amount of enzyme which liberated 1 µmol of fatty acids per min under assayed conditions. Calculation for the specific activity of lipase is based on equations (2) and (3) [22].…”

Covalent Immobilization of Lipase on Bacterial Cellulose Membrane and Nanocellulose

“…So many studies report that lipases com be physically immobilized, in which the enzyme is adsorbed on the support surface or network [17][18][19][20] . Enzyme immobilization had made laboratory and even industrial catalysis a more reliable processes, for example, due to the relative high cost to recovery these proteins from processed broth 21 .…”