Use of light scattering as a detector principle in liquid chromatography

Stołyhwo, Andrzej; Colin, Henri; Guiochon, Georges

doi:10.1016/s0021-9673(01)96693-2

Cited by 192 publications

(85 citation statements)

References 16 publications

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“…Quantitative Assessment of Ag + /HPLC/LLSD Chromatograms As reported earlier (Stolyhwo et al 1983(Stolyhwo et al , 1984(Stolyhwo et al , 1985(Stolyhwo et al , 1987, the LLSD signal was not linearly related to the mass or concentration of the analyte. After the technical improvements of the LLSD described above, the following simplified relationship may be used for preliminary assessment of the content of trans FA in analyzed fats:…”

Section: Gc Analysis Of Separated Fractionsmentioning

confidence: 56%

“…In contrast, solvents that could preferentially be used for HPLC-gradient elution of fatty acid methyl ester (FAME) and triacyloglycerols are not UV-transparent below 220 nm. The laser light scattering detector (LLSD) described earlier (Stolyhwo et al 1983(Stolyhwo et al , 1984(Stolyhwo et al , 1985(Stolyhwo et al , 1987 with several recent technical modifications, appears to be a very useful detecting device for use with both silver-loaded or RP columns in gradient elution HPLC. The merits of LLSD detectors were emphasized in the AOCS Ce 1g-96 instruction for detecting trans-isomers by silver ionexchange HPLC.…”

Section: Determination Of Trans-fatty Acids In Alimentary Fatsmentioning

confidence: 99%

“…The previously reported experimental design of LLSD (Stolyhwo et al 1983(Stolyhwo et al , 1984 was substantially modified. In brief, a laser diode (5 mW at 670 nm; Toshiba, Japan) was used at a controlled working temperature (40±2°C) in place of a He-Ne laser.…”

Section: Instrumentation and Proceduresmentioning

confidence: 99%

“…A 4-mm wide glass fiber optics (Glass Works, Bialystok, Poland) for transmission of scattered radiation and a photomultiplier (R-372HA, Hammamatsu, Japan) were used together with a chopper-stabilized operational amplifier (CMOS). The essential difference between the ELSD (Varex Corp. USA) and the locally made LLSD (Stolyhwo et al 1983(Stolyhwo et al , 1984(Stolyhwo et al , 1985(Stolyhwo et al , 1987 consisted of optimizing the LLSD for the lowest possible work temperature at which the eluate from the column was nebulized and the solvents evaporated. That aim was accomplished by restricting the maximum flow rate to the nebulizer to 0.2 mL/min (stream splitter with controlled resistance to flow used before the nebulizer).…”

Section: Instrumentation and Proceduresmentioning

confidence: 99%

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An Improved Silver Ion HPLC Combined with Capillary Gas Chromatography of cis/trans Fatty Acids in Alimentary Fats and Human Milk Fat

Stołyhwo

Rutkowska

2012

Food Anal. Methods

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A technique of analysis is described using a silver ion-loaded HPLC (Ag + /HPLC) column, gradient elution, and a laser light scattering detector followed by capillary gas chromatography (GC) for the determination of the fatty acid methyl ester (FAME) composition of rapeseed oil, the respective hydrogenated fat, some dietary fats, and human milk fat. With the use of Ag + /HPLC, FAMEs were separated into the following classes: saturated, trans-monounsaturated, cis-monounsaturated, trans-trans-di-unsaturated, trans-cisdi-unsaturated, cis-cis-di-unsaturated, and higher unsaturated FAMEs. Within each class, partial separation of positional isomers was also possible. Fractions of the eluate containing trans and cis C18:1 were separately collected and re-analyzed by GC in order to quantitate the positional isomers eight trans and nine cis in rapeseed oil and in human milk fat. The profiles of eight trans positional isomers in human milk fat and in hydrogenated rapeseed oil were alike but entirely different from that of six C18:1 trans isomers in bovine milk fat. Out of nine C18:1 cis isomers found in hydrogenated fat, only C18:1-9c and C18:1-11c were found in both human and bovine milk fat which may indicate some discrimination which occurs for certain isomers in fatty acid metabolism.

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Section: Gc Analysis Of Separated Fractionsmentioning

confidence: 56%

Section: Determination Of Trans-fatty Acids In Alimentary Fatsmentioning

confidence: 99%

Section: Instrumentation and Proceduresmentioning

confidence: 99%

Section: Instrumentation and Proceduresmentioning

confidence: 99%

See 2 more Smart Citations

An Improved Silver Ion HPLC Combined with Capillary Gas Chromatography of cis/trans Fatty Acids in Alimentary Fats and Human Milk Fat

Stołyhwo

Rutkowska

2012

Food Anal. Methods

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“…Evaporative light scattering detector (ELSD) [10][11][12] has been increasingly used and seems to offer a useful alternative to conventional methods in the quality control of TCM. Unlike UV detection, ELSD allows the determination of nonchromophoric compounds without preceding derivatization, and thus shows good compatibility to the multi-components of complex analytes such as TCM.…”

mentioning

confidence: 99%

Simultaneous Determination of Major Bioactive Components in <i>Qingkailing</i> Injection by High-Performance Liquid Chromatography with Evaporative Light Scattering Detection

Yan

Xin

Luo

et al. 2005

CHEMICAL & PHARMACEUTICAL BULLETIN

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High-performance liquid chromatography with evaporative light scattering detection (HPLC/ELSD) was established for simultaneous determination of seven major bioactive components of Qingkailing injection including adenosine, geniposide, chlorogenic acid, baicalin, ursodeoxycholic acid, cholic acid, and hyodeoxycholic acid. The proposed method was applied to analyze ten various Qingkailing injections and produced data with acceptable linearity, repeatability, precision and accuracy having a limit of detection (LOD) of 10-50 ng. In comparison with UV detection, HPLC/ELSD permits the determination of non-chromophoric compounds without prior derivatization, and shows good compatibility to the multi-components of complex analytes. The proposed method is a useful alternative for routine analysis in the quality control of traditional Chinese medicine.

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Chromatographic methods in the monitoring of lipase-catalyzed interesterification

Mu¹,

Kalo

et al. 2000

Eur. J. Lipid Sci. Technol.

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Structured lipids containing special fatty acids were produced via lipase‐catalyzed interesterification. Chromatographic methods were modified for the analysis of the acylglycerol content of the selected interesterified products, resulting from different reacting substrate mixtures and reaction systems. A modified normal‐phase high performance liquid chromatographic method with evaporative light‐scattering detection was used in the analysis of the diacylglycerols formed as intermediate products during interesterification. One of the advantages of the method was the low solvent consumption through the use of a narrow‐bore silica column. Another advantage was in the speed of the analysis. Using a suitable solvent gradient, both diacylglycerol and triacylglycerol components were separated by reversed‐phase high performance liquid chromatography within 50 min, providing both the diacylglycerol content and the triacylglycerol composition. High‐temperature gas chromatography was also used to separate both diacylglycerol and triacylglycerol molecular species after trimethylsilylation of the structured lipid samples. Good agreements in the values obtained for the concentrations of diacylglycerols and the triacylglycerol compositions were apparent with the different chromatographic methods.

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Use of light scattering as a detector principle in liquid chromatography

Cited by 192 publications

References 16 publications

An Improved Silver Ion HPLC Combined with Capillary Gas Chromatography of cis/trans Fatty Acids in Alimentary Fats and Human Milk Fat

An Improved Silver Ion HPLC Combined with Capillary Gas Chromatography of cis/trans Fatty Acids in Alimentary Fats and Human Milk Fat

Simultaneous Determination of Major Bioactive Components in <i>Qingkailing</i> Injection by High-Performance Liquid Chromatography with Evaporative Light Scattering Detection

Chromatographic methods in the monitoring of lipase-catalyzed interesterification

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