Use of Leu M1 and antiepithelial membrane antigen monoclonal antibodies for diagnosing Hodgkin's disease.

Jack, Andrew; Cunningham, Dawn; Soukop, M.; Liddle, C N; Lee, F D

doi:10.1136/jcp.39.3.267

Cited by 29 publications

(3 citation statements)

References 8 publications

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“…Variable staining of Reed-Sternberg cells in the L&H predominance subtype of Hodgkin's disease is reported, Innes & Frierson 1985, Norton & Isaacson 1985, Jack et al 1986, Hsu et al. 1986, Kornstein et al 1986).…”

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confidence: 80%

An immunocytochemical study of T‐cell lymphomas using monoclonal and polyclonal antibodies effective in routinely fixed wax embedded tissues

Norton

Isaacson

1986

Histopathology

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Formalin fixed and paraffin wax embedded tissue from 24 cases of T-cell lymphoma diagnosed using immunocytochemistry on cryostat sections was examined using a panel of eight monoclonal and three polyclonal antisera. The monoclonal antibodies UCHL1 and MT1 proved to be comparable and reliable markers of neoplastic cells in T-cell lymphomas. The B-cell specific marker, MB1, strongly stained all cells in two cases of pleomorphic large cell T-cell lymphoma, large cells in two cases of pleomorphic mixed medium and large cell lymphoma, and isolated clusters of blast cells in four cases of T-zone and angioimmunoblastic lymphadenopathy-like T-cell lymphoma. The cells stained by MB1 expressed T suppressor/cytotoxic surface markers on frozen section. Epithelial membrane antigen, as detected by a polyclonal anti-EMA and the monoclonal antibody HMFG2, was expressed in 36% of tumours especially those of monomorphic large cell and pleomorphic large cell phenotype. Single granules or finely dispersed cytoplasmic granularity was seen in four tumours using the anti-granulocyte reagent Leu M1. Tumour cells in one case stained in a pattern identical to Reed-Sternberg cells in Hodgkin's disease. Granular alpha-1-antitrypsin staining was found in 10 cases of pleomorphic large cell and monomorphic large cell lymphoma. No staining was observed using anti-lysozyme or the monoclonal macrophage specific marker Mac411. Monomorphic and pleomorphic large cell lymphomas tended to show a common immunophenotype with the majority of cells co-expressing alpha-1-antitrypsin HLA-DR and epithelial membrane antigen. Scattered large transformed blast cells in cases of angioimmunoblastic lymphadenopathy-like T-cell lymphomas and T-zone lymphomas shared a similar immunophenotype with the large cell lymphomas. Using a panel of monoclonal antibodies effective in paraffin embedded tissue, diagnostically useful staining profiles which correlate with the morphological phenotype can be established in T-cell lymphomas.

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confidence: 80%

An immunocytochemical study of T‐cell lymphomas using monoclonal and polyclonal antibodies effective in routinely fixed wax embedded tissues

Norton

Isaacson

1986

Histopathology

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“…',2 Immunohistochemistry for cytokeratins in particular is widely used in the diagnosis of anaplastic tumours, where perhaps the commonest problem is differentiation of carcinoma from lymphoma.3 Epithelial membrane antibodies are also usef~1,4~~ although they have a narrower spectrum of reactivity than the cytokeratins and give occasional staining of cells of lymphoid origin. 6 The cytokeratins comprise a closely related group of 19 polypeptides as described by Moll eta'.,' and many authors refer to these by their 'Moll number' as determined by two-dimensional gel electrophoresis. Differing types of epithelia have characteristic cytokeratin expression patterns which are generally shared by tumours derived from such epithelia.…”

Section: Introductionmentioning

confidence: 99%

NCL‐5D3: A new monoclonal antibody recognizing low molecular weight cytokeratins effective for immunohistochemistry using fixed paraffin‐embedded tissue

Angus

Purvis

Stock

et al. 1987

The Journal of Pathology

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Production of a new monoclonal antibody designated NCL-5D3 is described. The antibody recognizes several low molecular weight cytokeratins, in particular cytokeratin Moll number 8 as determined by immunoblotting studies, and is highly effective for immunocytochemistry using routinely processed paraffin-embedded material. Staining is enhanced by prior treatment of the sections with trypsin. Assessment using a wide variety of normal and neoplastic tissue indicates reactivity with all tissues of simple or glandular epithelial origin, and in addition with many squamous carcinomas. Thus the antibody should prove of value in diagnostic histopathology.

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“…A monoclonal antibody raised against a human monocyte cell line (U937) and designated anti Leu M1 is known to identify a differentiation antigen (CD 15) on human myelomonocytic cells,' and to recognize determinants of Reed -Sternberg cells of most variants of Hodgkin's disease (HD).2. 3 The CD15 antigen has recently also been detected on and within neoplastic cells of several forms of T ccll lymph~ma/leukaemia~-~ and on in vitro mitogen-stimulated, normal T helper/inducer lymphocytes. ' During the course of our immunohistological investigations of extra-nodal lymphoma, we noted consistent, specific staining of structures such as the salivary gland and gastrointestinal epithelial cells by the anti Leu M1 antibody.…”

Section: Introductionmentioning

confidence: 99%

Reaction of monoclonal anti Leu M1—a myelomonocytic marker (CD15)—with normal and neoplastic epithelia

Sewell

Jaffray

Thompson

1987

The Journal of Pathology

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Use of Leu M1 and antiepithelial membrane antigen monoclonal antibodies for diagnosing Hodgkin's disease.

Cited by 29 publications

References 8 publications

An immunocytochemical study of T‐cell lymphomas using monoclonal and polyclonal antibodies effective in routinely fixed wax embedded tissues

An immunocytochemical study of T‐cell lymphomas using monoclonal and polyclonal antibodies effective in routinely fixed wax embedded tissues

NCL‐5D3: A new monoclonal antibody recognizing low molecular weight cytokeratins effective for immunohistochemistry using fixed paraffin‐embedded tissue

Reaction of monoclonal anti Leu M1—a myelomonocytic marker (CD15)—with normal and neoplastic epithelia

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