Use of Lentinan and Fluopimomide to Control Cotton Seedling Damping-Off Disease Caused by Rhizoctonia solani

Abstract

Lentinan (LNT) is a natural and functional polysaccharide isolated from Lentinus edodes fruiting bodies, which functions in stimulating the plant immune response, improving plant disease resistance and regulating plant growth. This study explores the use of LNT as a plant growth regulator and attractant in cotton production. After treatment with LNT, the content of malondialdehyde (MDA) in cotton seeds decreased, whereas the activities of polyphenol oxidase (PPO), superoxide dismutase (SOD) and peroxidase (POD) in leaves increased significantly. LNT also promoted the growth and development of cotton plants and significantly reduced the incidence of cotton damping-off disease. The relative expression of salicylic acid pathway-related genes in cotton also increased significantly. The prevention mechanism of fluopimomide was also evaluated, and the result showed lower EC50 values and was effective in controlling cotton seedling disease caused by Rhizoctonia solani in both greenhouse experiments and field trials. The use of LNT and fluopimomide in controlling cotton seedling damping-off disease showed a synergistic effect in field trials. These results will provide a new insight into the agricultural application of LNT as a biological fungicide in the field of biological controls.

A chitosan‐coated lentinan‐loaded calcium alginate hydrogel induces broad‐spectrum resistance to plant viruses by activating Nicotiana benthamiana calmodulin‐like (CML) protein 3

“…Polysaccharide immunity inducers, such as amino oligosaccharides, CTS, chitin and LNT, have been widely used in controlling plant virus diseases (Zhao et al, 2007). LNT, as a biological polysaccharide, has multiple functions that confer disease resistance, such as closing plant stomata, inducing the expression of plant resistance genes and improving plant defence‐related enzyme activity, such as superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) (Sun et al, 2022; Yin et al, 2010; Zhao et al, 2007). However, previously generated hydrogels loaded with LNT are confined in field application due to the limited release time of LNT and its instability in the complex field environment (Xiang et al, 2019).…”

Characterization and control of Rhizoctonia solani affecting lucky bamboo (Dracaena sanderiana hort. ex. Mast.) using some bioagents

“…During the period of March–May 2019, 226 samples of naturally infected lucky bamboo showing characteristic symptoms of severe root rot, basal stem rot, and wilt disease complex were randomly collected from 630 lucky bamboo plants surveyed at various locations at retail stores, shops, and nurseries in Alexandria, El-Behera, and Giza governorates, Egypt. In all the mentioned sites, the percentages of disease infection were recorded and calculated 30 , 31 using the ratings shown in Table 1 .…”

Integrated non‐targeted and targeted metabolomics analysis reveals the mechanism of inhibiting lignification and optimizing the quality of pea sprouts by combined application of nano‐selenium and lentinans

“…Although LNT does not possess direct bactericidal activity, it stimulates secondary metabolism and the antioxidant system of crops to increase their disease resistance. 16 Additionally, it acts as a foliar fertilizer and has been used in tobacco, [17][18][19] cotton, 20 and wheat 21 as an eco-friendly biological agent to control plant diseases. Few studies, nevertheless, have investigated how LNT as a foliar fertilizer affects crop quality.…”