Use of laser‐scanning confocal microscopy in the detection of diagenesis in bone

Abstract

This research demonstrates the value of laser scanning confocal microscopy (LSCM) as a research tool in osteological studies, and diagenetic studies in particular. LSCM combines properties of light and scanning electron microscopy using laser light to excite fluorophores throughout the z-axis, developing a 3-D image. Using differen- Highlights• Test use of LSCM and bone focusing on microstructure, protein isolation, and diagenetic alteration.• Pig samples exhibited differences in wavelength registration/intensity and isolation of organic matrix.• Human sample found focal isolation of diagenetic alteration using toluidine blue.• Possible to detect bone protein using differential staining and wavelength registration.• Digenetic changes can be seen in a three-dimensional view.