Use of laser microdissection greatly improves the recovery of DNA from sperm on microscope slides

Elliott, Keith; Hill, D.S; Lambert, Cathy; Burroughes, T.R; Gill, Peter

doi:10.1016/s0531-5131(03)01509-7

Cited by 30 publications

(36 citation statements)

References 7 publications

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“…This may be due to adherence of female DNA to the spermatozoa [7,16]. Despite the presence of female DNA in one of the samples after LPC, the male DNA profile was interpreted easily.…”

Section: Discussionmentioning

confidence: 96%

“…In one of the samples a few extra alleles from the female donor were recovered. This mixture may be due to the adherence of female DNA from lysed epithelial cells to spermatozoa [7,16]. Despite the presence of female DNA, the male DNA profile was easily interpreted.…”

Section: Automated Detection Of Spermatozoamentioning

confidence: 99%

“…Elliot et al showed that LCM outperforms differential extraction for the recovery of DNA from sperm cells, especially in cases where only a few sperm cells are present [7]. LCM was also used for the isolation of male cells in a male/female cell mixture [8,9].…”

Section: Introductionmentioning

confidence: 99%

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Automatic detection of spermatozoa for laser capture microdissection

et al. 2008

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In sexual assault crimes, differential extraction of spermatozoa from vaginal swab smears is often ineffective, especially when only a few spermatozoa are present in an overwhelming amount of epithelial cells. Laser capture microdissection (LCM) enables the precise separation of spermatozoa and epithelial cells. However, standard spermstaining techniques are non-specific and rely on sperm morphology for identification. Moreover, manual screening of the microscope slides is time-consuming and laborintensive. Here, we describe an automated screening method to detect spermatozoa stained with Sperm HY-LITER™. Different ratios of spermatozoa and epithelial cells were used to assess the automatic detection method. In addition, real postcoital samples were also screened. Detected spermatozoa were isolated using LCM and DNA analysis was performed. Robust DNA profiles without allelic dropout could be obtained from as little as 30 spermatozoa recovered from postcoital samples, showing that the staining had no significant influence on DNA recovery.

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“…This may be due to adherence of female DNA to the spermatozoa [7,16]. Despite the presence of female DNA in one of the samples after LPC, the male DNA profile was interpreted easily.…”

Section: Discussionmentioning

confidence: 96%

Section: Automated Detection Of Spermatozoamentioning

confidence: 99%

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Automatic detection of spermatozoa for laser capture microdissection

et al. 2008

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“…The different cell types can be recognized by morphological characteristics, various chemical staining or fluorescence labeling techniques. These methods allow to establish a clear DNA profile from few cells present in a mixture samples that otherwise had not be detected while swabbed by the major component and not detectable in the profile ( Elliott et al, 2003;Anslinger et al, 2005;Anoruo et al, 2007 ;Sanders et al, 2006). With laser micro dissection techniques ( Anslinger et al, 2007;Vandewoestyne et al, 2009), it has been shown that cells derived from a male contributor can be analyzed separately from those derived from a female contributor after morphological or fluorescent labeling identification.…”

Section: Collecting Methodologiesmentioning

confidence: 99%

Avoiding Errors and Pitfalls in Evidence Sampling for Forensic Genetics

Ludes¹,

Keyser²

2011

Forensic Medicine - From Old Problems to New Challenges

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“…The LCM method has been used to successfully isolate sperm cells in sexual assault evidence [43,107]. In order to isolate the cells, the sample is spread over a microscope slide and a thin plastic film is placed on top of it.…”

Section: B8 Laser Microdissectionmentioning

confidence: 99%

A Novel Method for Rapid and Selective Extraction of Male DNA from Rape Kits using Alkaline Lysis and Pressure Cycling Technology (PCT)

Nori¹

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Use of laser microdissection greatly improves the recovery of DNA from sperm on microscope slides

Cited by 30 publications

References 7 publications

Automatic detection of spermatozoa for laser capture microdissection

Automatic detection of spermatozoa for laser capture microdissection

Avoiding Errors and Pitfalls in Evidence Sampling for Forensic Genetics

A Novel Method for Rapid and Selective Extraction of Male DNA from Rape Kits using Alkaline Lysis and Pressure Cycling Technology (PCT)

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