Use of labeled tomato lectin for imaging vasculature structures

Robertson, Richard T.; Levine, Samantha T.; Haynes, Sherry M.; Gutierrez, Paula; Baratta, Janie; Tan, Zhiqun; Longmuir, Kenneth J.

doi:10.1007/s00418-014-1301-3

Cited by 154 publications

(119 citation statements)

References 31 publications

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“…Instead, we visualized the blood vessels by perfusing FITC-lectin prior to sacrificing the animals. FITC-lectin stains vessels by binding to glycoproteins of endothelial plasmalemma [71] and has been used to locate endothelial cells in studies involving NPs [72, 73]. Due to the leakiness of tumor vasculature, FITC-lectin stain in tumors showed a more diffuse pattern than in liver or spleen, consistent with the literature [74, 75].…”

Section: Resultssupporting

confidence: 60%

Surface modification of polymer nanoparticles with native albumin for enhancing drug delivery to solid tumors

et al. 2018

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Albumin is a promising surface modifier of nanoparticulate drug delivery systems. Serving as a dysopsonin, albumin can protect circulating nanoparticles (NPs) from the recognition and clearance by the mononuclear phagocytic system (MPS). Albumin may also help transport the NPs to solid tumors based on the increased consumption by cancer cells and interactions with the tumor microenvironment. Several studies have explored the benefits of surface-bound albumin to enhance NP delivery to tumors. However, it remains unknown how the surface modification process affects the conformation of albumin and the performance of the albumin-modified NPs. We use three different surface modification methods including two prevalent approaches (physisorption and interfacial embedding) and a new method based on dopamine polymerization to modify the surface of poly(lactic-co-glycolic acid) NPs with albumin and compare the extent of albumin binding, conformation of the surface-bound albumin, and biological performances of the albumin-coated NPs. We find that the dopamine polymerization method preserves the albumin structure, forming a surface layer that facilitates NP transport and drug delivery into tumors via the interaction with albumin-binding proteins. In contrast, the interfacial embedding method creates NPs with denatured albumin that offers no particular benefit to the interaction with cancer cells but rather promotes the MPS uptake via direct and indirect interactions with scavenger receptor A. This study demonstrates that the surface-bound albumin can bring distinct effects according to the way they interact with NP surface and thus needs to be controlled in order to achieve favorable therapeutic outcomes.

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Section: Resultssupporting

confidence: 60%

Surface modification of polymer nanoparticles with native albumin for enhancing drug delivery to solid tumors

et al. 2018

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“…The use of immunohistochemistry to detect endothelial related markers has commonly been used, particularly Von Wildebrand factor and CD-31 or PECAM-1, a protein found on the surface of a variety of circulating blood cells and also on the surface of endothelial cells. 108 Recently, the use of intravascularly injected tomato lectin has been used to provide a detailed image of cerebral (and other) vascular structures (see Figure 2), 109 and use of a zinc fixative before immunohistochemistry for CD-31 in thick vibratome sections has been shown to be helpful in visualizing brain vasculature. 110 A common approach used intravascular injections of Evans blue to assess BBB leakage.…”

Section: Methods Of Detection Of Vascular Changes Post-tbimentioning

confidence: 99%

“…Our laboratory has begun a series of preliminary experiments, studying the effects of varied impact parameters of TBI on cerebral cortical vascular elements, as well as the possible ''leakiness'' of those cortical blood vessels. These studies required a combination of techniques, including: (1) intravascular injections of fluorescently labeled lectin 109 as a general label for the endothelium of cerebral cortical blood vessels, combined with (2) intravascular injections of tracers, including Evans blue, labeled albumin, or dextrans of varied molecular weights to study the possible movement of molecules of varied sizes from circulating blood into brain parenchyma. Injections of lectins and tracers were made at varied times, ranging from just prior to TBI injury to one month post injury.…”

Section: Comparison Of Single Vs Multiple Impactsmentioning

confidence: 99%

Effects of concussion on the blood–brain barrier in humans and rodents

Sahyouni

Gutierrez

Gold

et al. 2017

Journal of Concussion

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Traumatic brain injury and the long-term consequences of repeated concussions constitute mounting concerns in the United States, with 5.3 million individuals living with a traumatic brain injury-related disability. Attempts to understand mechanisms and possible therapeutic approaches to alleviate the consequences of repeat mild concussions or traumatic brain injury on cerebral vasculature depend on several aspects of the trauma, including: (1) the physical characteristics of trauma or insult that result in damage; (2) the time ''window'' after trauma in which neuropathological features develop; (3) methods to detect possible breakdown of the blood-brain barrier; and (4) understanding different consequences of a single concussion as compared with multiple concussions. We review the literature to summarize the current understanding of blood-brain barrier and endothelial cell changes post-neurotrauma in concussions and mild traumatic brain injury. Attention is focused on concussion and traumatic brain injury in humans, with a goal of pointing out the gaps in our knowledge and how studies of rodent model systems of concussion may help in filling these gaps. Specifically, we focus on disruptions that concussion causes to the blood-brain barrier and its multifaceted consequences. Importantly, the magnitude of post-concussion blood-brain barrier dysfunction may influence the time course and extent of neuronal recovery; hence, we include in this review comparisons of more severe traumatic brain injury to concussion where appropriate. Finally, we address the important, and still unresolved, issue of how best to detect possible breakdown in the blood-brain barrier following neurotrauma by exploring intravascular tracer injection in animal models to examine leakage into the brain parenchyma.

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“…To visualize the blood vessels sectioned tendonss were stained with Verhoeff and lectin [26-29] stains. For Verhoeff staining sections were rehydrated, stained with working Verhoeff solution (25089-1, Polysciences, Inc.) for 20 minutes, differentiated using 1% tartrazine in 1% citric acid for 3 mins, and counterstained with Van Gieson’s (25089-1, Polysciences Inc.) solution for 3 mins.…”

Section: Methodsmentioning

confidence: 99%

Effects of PDGF-BB delivery from heparinized collagen sutures on the healing of lacerated chicken flexor tendon in vivo

et al. 2017

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Flexor tendon lacerations are traditionally repaired by using non-absorbable monofilament sutures. Recent investigations have explored to improve the healing process by growth factor delivery from the sutures. However, it is difficult to conjugate growth factors to nylon or other synthetic sutures. This study explores the performance of a novel electrochemically aligned collagen suture in a flexor tendon repair model with and without platelet derived growth factor following complete tendon laceration in vivo. Collagen suture was fabricated via electrochemical alignment process. Heparin was covalently bound to electrochemically aligned collagen sutures (ELAS) to facilitate affinity bound delivery of platelet-derived growth factor-BB (PDGF-BB). Complete laceration of the flexor digitorum profundus in the third digit of the foot was performed in 36 skeletally mature White Leghorn chickens. The left foot was used as the positive control. Animals were randomly divided into three groups: control specimens treated with standard nylon suture (n=12), specimens repaired with heparinated ELAS suture without PDGF-BB (n=12) and specimens repaired with heparinated ELAS suture with affinity bound PDGF-BB (n=12). Specimens were harvested at either 4 weeks or 12 weeks following tendon repair. Differences between groups were evaluated by the degree of gross tendon excursion, failure load/stress, stiffness/modulus, absorbed energy at failure, elongation/strain at failure. Quantitative histological scoring was performed to assess cellularity and vascularity. Closed flexion angle measurements demonstrated no significant differences in tendon excursion between the study groups at 4 or 12 weeks. Biomechanical testing showed that the group treated with PDGF-BB bound heparinated ELAS suture had significantly higher stiffness and failure load (p<0.05) at 12-weeks relative to both heparinated ELAS suture and nylon suture. Similarly, the group treated with PDGF-BB bound suture had significantly higher ultimate tensile strength and Young’s modulus (p<0.05) at 12-weeks relative to both ELAS suture and nylon suture. Compared to nylon controls, heparinized ELAS with PDGF-BB improved biomechanics and vascularity during tendon healing by 12-weeks following primary repair. The ability of ELAS to deliver PDGF-BB to the lacerated area of tendon presents investigators with a functional bioinductive platform to improve repair outcomes following flexor tendon repair.

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Use of labeled tomato lectin for imaging vasculature structures

Cited by 154 publications

References 31 publications

Surface modification of polymer nanoparticles with native albumin for enhancing drug delivery to solid tumors

Surface modification of polymer nanoparticles with native albumin for enhancing drug delivery to solid tumors

Effects of concussion on the blood–brain barrier in humans and rodents

Effects of PDGF-BB delivery from heparinized collagen sutures on the healing of lacerated chicken flexor tendon in vivo

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