Use of ISSR markers to assess genetic diversity of African edible seeded Citrullus lanatus landraces

Djè, Y.; Tahi, C. G.; Bi, Arsène Irié Zoro; Baudoin, Jean-Pierre; Bertin, Pierre

doi:10.1016/j.scienta.2009.12.020

Cited by 28 publications

(11 citation statements)

References 25 publications

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“…Both inter-simple sequence repeat (ISSR) and SSR markers based on the polymerase chain reaction (PCR) are commonly used for measuring genetic diversity [ 16 , 17 ] and for cultivar identification [ 18 , 19 ]. ISSR analyses are easy to perform, are inexpensive, and do not require prior genetic information [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity and Identification of Chinese-Grown Pecan Using ISSR and SSR Markers

Jia

Wang

Zhai³

et al. 2011

Molecules

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Pecan is an important horticultural nut crop originally from North America and now widely cultivated in China for its high ecological, ornamental and economic value. Currently, there are over one hundred cultivars grown in China, including introduced American cultivars and Chinese seedling breeding cultivars. Molecular markers were used to assess the genetic diversity of these cultivars and to identify the pedigrees of fine pecan plants with good characteristics and no cultivar-related data. A total of 77 samples grown in China were studied, including 14 introduced cultivars, 12 domestic seedling breeding cultivars, and 49 fine pecan plants with no cultivar data, together with Carya cathayensis and Juglans nigra. A total of 77 ISSR and 19 SSR primers were prescreened; 10 ISSR and eight SSR primers were selected, yielding a total of 94 amplified bands (100% polymorphic) in the range of 140–1,950 bp for the ISSR and 70 amplified bands (100% polymorphic) in the range of 50–350 bp for SSR markers. Genetic diversity analyses indicated Chinese-grown pecan cultivars and fine plants had significant diversity at the DNA level. The dengrograms constructed with ISSR, SSR or combined data were very similar, but showed very weak grouping association with morphological characters. However, the progeny were always grouped with the parents. The great diversity found among the Chinese cultivars and the interesting germplasm of the fine pecan plants analyzed in this study are very useful for increasing the diversity of the pecan gene pool. All 77 accessions in this study could be separated based on the ISSR and SSR fingerprints produced by one or more primers. The results of our study also showed that ISSR and SSR techniques were both suitable for genetic diversity analyses and the identification of pecan resources.

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Section: Introductionmentioning

confidence: 99%

Genetic Diversity and Identification of Chinese-Grown Pecan Using ISSR and SSR Markers

Jia

Wang

Zhai³

et al. 2011

Molecules

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“…Different estimates for the degrees of genetic variation were obtained from these reports, reflecting the differences in the selected genotypes and marker systems. Several studies showed that it was difficult to detect DNA polymorphism in cultivated watermelon using RAPD (Lee et al, 1996;Levi et al, 2001) and ISSR (Djè et al, 2010), implying a lack of genetic variability in cultivated germplasms. Of the DNA markers used, SSR has been the most informative and has proven to be useful for accession discrimination and diversity assessment in cultivated watermelons.…”

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confidence: 99%

Comparative analysis of genetic diversity among Chinese watermelon germplasmsusing SSR and SRAP markers, and implications for future genetic improvement

Wang¹,

Li²,

Hu³

et al. 2015

Turk J Agric For

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The genetic diversity of watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai] in China, the world's largest producer of watermelon fruits, has not been examined. Two molecular markers, sequence-related amplified polymorphism (SRAP) and simple sequence repeat (SSR), were used to investigate the genetic variation and genetic relationship among 54 Chinese watermelon accessions, as well as 7 accessions from Africa, the United States, and Japan. SRAP assay generated 312 bands, of which 163 were polymorphic, while SSR assay detected 101 alleles with 87 polymorphic alleles and 114 genotypes. A comparative analysis of the diversity index, effective multiplex ratio, and marker index of the 2 markers showed higher efficiency of SRAP markers in watermelon germplasm fingerprinting. The Mantel test resulted in a high correlation between SRAP and SSR data with a value of γ = 0.863, revealing a good fit between the 2 marker systems. Genetic diversity among the accession set was estimated by a construction of dendrograms using SRAP, SSR, or SRAP-SSR combined data. There was clear consistency between the 3 dendrograms on the positioning of most accessions. The study demonstrated that most Chinese cultivated germplasms (CCGs) were clustered independently of their geographical distribution and horticultural classification, and had a narrow genetic base; most CCGs and foreign cultivars had a high genetic closeness and showed similar lineages; and the 4 wild accessions were the most genetically diverse, followed by the small-fruited accessions from northern China and edible-seeded accessions. We concluded that CCGs lacked genetic variation, and we offer a means of introducing new genetic diversity into the watermelon germplasm pool in China, which is essential to maximizing productivity in watermelon.

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“…However, utilizing 22 RAPD primers, Solmaz et al (2010) had demonstrated 60.6% polymorphism in 303 Turkish watermelon accessions that is similar to our results. Comparable results have been obtained by Djè et al (2010) revealing 97.7% polymorphism in African indigenous watermelon landraces with 20 ISSR primers. Although RAPD markers are randomly distributed in the genome and ISSR distribution is comparatively more restricted, ISSR shows greater percentage polymorphism as compared to RAPD.…”

Section: Discussionmentioning

confidence: 60%

“…Inter-Simple Sequence Repeat (ISSR) marker system is one more PCR-based organization having extensive relevance for different species, apart from the accessibility of information regarding their genome series (Gui et al 2007;Kurane et al 2009;Shi et al 2010). This marker system has also been verified as more reproducible and consistent, showing profuse polymorphism in comparison to RAPD, in watermelon species (Levi et al 2004Djè et al 2010;Yang et al 2010;Huang et al 2011).…”

Section: Introductionmentioning

confidence: 81%

Genetic diversity and population structure of watermelon (Citrullus sp.) genotypes

et al. 2019

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Genetic polymorphism amid plant species is a crucial factor for plant improvement and maintaining their biodiversity. Evaluation of genetic diversity amongst plant species is significant to deal with the environmental stress conditions and their effective involvement in the breeding programs. Hence, in present study, an attempt has been made towards the genetic assessment of individual and bulked populations of 25 watermelon genotypes, belonging to Citroides (citron watermelon) and Lanatus (dessert watermelon) group from Konya, Thrace, Turkmenistan, Saudi Arabia and Turkey. The employed Random Amplified Polymorphic DNA (RAPD) and Inter-Simple Sequence Polymorphism (ISSR) marker systems provided 69.4 and 95.4% polymorphisms, respectively. Different clustering methods showed clear grouping of the genotypes based on the geographical origin and species. Citron genotypes from Turkmenistan stood apart from all the Turkish Lanatus genotypes. However, Saudi Arab Lanatus genotype grouped with native Turkish varieties indicating the genetic linkage. Among all the Turkmenistan Citron genotypes, Turkmenistan-11 was the most distinct form. Moreover, sufficient genetic variation was found between the commercial and native Lanatus genotypes of Turkey as well as Citron genotypes of Turkmenistan. Hence, it will be beneficial to include these genotypes in the future breeding programs to transfer disease-resistant alleles from Citron to Lanatus genotypes.

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Use of ISSR markers to assess genetic diversity of African edible seeded Citrullus lanatus landraces

Cited by 28 publications

References 25 publications

Genetic Diversity and Identification of Chinese-Grown Pecan Using ISSR and SSR Markers

Genetic Diversity and Identification of Chinese-Grown Pecan Using ISSR and SSR Markers

Comparative analysis of genetic diversity among Chinese watermelon germplasmsusing SSR and SRAP markers, and implications for future genetic improvement

Genetic diversity and population structure of watermelon (Citrullus sp.) genotypes

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