Use of isotopes in the diagnosis of hematopoietic disorders

Shreeve, Walton Wallace

doi:10.1016/j.exphem.2007.01.027

Cited by 10 publications

(4 citation statements)

References 17 publications

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“…In the present study, we propose a new approach to this issue exploiting the advantages of hybrid PET/CT technology. FDG imaging has been already used to document BM infiltration by cancer [2,[10][11][12][13] and to monitor the haematopoietic response to chemo-and radiotherapy in specific bone regions [14]. The aim of our methods was to extend this clinically validated tool to provide a first description of the extension and distribution of the BM throughout the whole skeleton of living humans.…”

Section: Introductionmentioning

confidence: 99%

Estimating the whole bone-marrow asset in humans by a computational approach to integrated PET/CT imaging

Sambuceti

Brignone

Marini

et al. 2012

Eur J Nucl Med Mol Imaging

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Section: Introductionmentioning

confidence: 99%

Estimating the whole bone-marrow asset in humans by a computational approach to integrated PET/CT imaging

Sambuceti

Brignone

Marini

et al. 2012

Eur J Nucl Med Mol Imaging

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“…19 Cobalt can be used in cancer chemotherapy 20 and, as its radioactive isotope, it is used in the diagnosis of hematopoietic disorders. 21 This element can be toxic and accumulates in the liver with a half-life of about 10 years. 22 Cobalt is known to form complexes with T, 14,23,24 with an affinity constant estimated to be close to that of iron (∼ 10 21 M).…”

Section: Introductionmentioning

confidence: 99%

Cobalt and the Iron Acquisition Pathway: Competition towards Interaction with Receptor 1

Chikh

Hémadi

Miquel

et al. 2008

Journal of Molecular Biology

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“…Indeed, T forms stable complexes with more than 40 different metals [11]. Such binding may play an important role in the transport and delivery of medical diagnostic radioisotopes such as 67 Ga 3+ [15], 60 Co 3+ [16] and [17], toxic metals such as Al 3+ [18,19], and therapeutic metal ions such as Ru 3+ and Ti 4+ [20][21][22]. Can these metals be incorporated via the iron-acquisition pathway?…”

Section: Introductionmentioning

confidence: 99%

Kinetics and thermodynamics of metal-loaded transferrins: transferrin receptor 1 interactions

Ha-Duong

Hémadi

Chikh

et al. 2008

Biochemical Society Transactions

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Transferrin receptor 1 (R) and human serum transferrin (T) are the two main actors in iron acquisition by the cell. R binds TFe(2) (iron-loaded transferrin), which allows its internalization in the cytoplasm by endocytosis. T also forms complexes with metals other than iron. In order to follow the iron-acquisition pathway, these metals should obey at least two essential rules: (i) formation of a strong complex with T; and (ii) interaction of this complex with R. In the present paper, we propose a general mechanism for the interaction of five metal-loaded Ts [Fe(III), Al(III), Bi(III), Ga(III) and Co(III)] with R and we discuss their potential incorporation by the iron-acquisition pathway. With iron- and cobalt-loaded Ts, the interaction of R takes place in two steps: the first is detected by the T-jump technique and occurs in the 100 micros range, whereas the second is slow and occurs in the hour range. Bi(III)- and Ga(III)-loaded Ts interact with R in a single fast kinetic step, which occurs in the 100-500 micros range. No interaction is detected between R and aluminium-saturated T. The fast steps are ascribed to the interaction of the C-lobe of metal-loaded T with the helical domain of R: dissociation constant, K'(1), of 0.50+/-0.07, 0.82+/-0.25, 4+/-0.4 and 1.10+/-0.12 microM for Fe(III), Co(III), Bi(III) and Ga(III) respectively. The second slow steps are ascribed to changes in the conformation of the protein-protein adducts which increase the stability to achieve, at thermodynamic equilibrium, an overall dissociation constant, K(1), of 2.3 and 25 nM for Fe(III) and Co(III) respectively. This last step occurs over several hours, whereas endocytosis takes place in several minutes. This implies that metal-loaded Ts are internalized with only the C-lobe interacting with R. This suggests that, despite a lower affinity for R when compared with TFe(2), some metal-loaded Ts can compete kinetically with TFe(2) for the interaction with R and thus follow the iron-acquisition pathway.

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Use of isotopes in the diagnosis of hematopoietic disorders

Cited by 10 publications

References 17 publications

Estimating the whole bone-marrow asset in humans by a computational approach to integrated PET/CT imaging

Estimating the whole bone-marrow asset in humans by a computational approach to integrated PET/CT imaging

Cobalt and the Iron Acquisition Pathway: Competition towards Interaction with Receptor 1

Kinetics and thermodynamics of metal-loaded transferrins: transferrin receptor 1 interactions

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