Use of intravoxel incoherent motion MRI to assess renal fibrosis in a rat model of unilateral ureteral obstruction

Cai, Xiang‐Ran; Yu, Jian; Zhou, Qingchun; Du, Bin; Feng, You‐Zhen; Liu, Xiaoling

doi:10.1002/jmri.25172

Cited by 37 publications

(38 citation statements)

References 29 publications

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“…13 IVIM-DWI has been used to detect dysfunction of transplanted kidney in human studies, 14,15 and evaluate renal fibrosis using animal models. [16][17][18] Moreover, cortical D, D*, and f showed a positive correlation with estimated glomerular filtration rate (eGFR) in transplanted kidneys. 14 These studies confirmed the feasibility of IVIM-DWI for the evaluation of renal function and microstructures, and the sclerosis of glomeruli, tubular atrophy, interstitial fibrosis, and interstitial inflammatory cell infiltration in CKD that possibly lead to the reduction of diffusion parameters.…”

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confidence: 99%

“…IVIM‐DWI is able to determine the pure molecule diffusion by calculating the diffusion coefficient of nonperfusion‐based molecular diffusion (D), and evaluate intravoxel capillary perfusion by calculating the diffusion coefficient of perfusion‐based molecular diffusion (D*), as well as the perfusion fraction (f) . IVIM‐DWI has been used to detect dysfunction of transplanted kidney in human studies, and evaluate renal fibrosis using animal models . Moreover, cortical D, D*, and f showed a positive correlation with estimated glomerular filtration rate (eGFR) in transplanted kidneys …”

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confidence: 99%

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Chronic kidney disease: Pathological and functional evaluation with intravoxel incoherent motion diffusion‐weighted imaging

Mao

Zhou

Zeng

et al. 2017

Magnetic Resonance Imaging

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confidence: 99%

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confidence: 99%

Chronic kidney disease: Pathological and functional evaluation with intravoxel incoherent motion diffusion‐weighted imaging

Mao

Zhou

Zeng

et al. 2017

Magnetic Resonance Imaging

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“…The UUO model has been widely employed in the study of renal fibrosis, especially for radiological research . However, UUO‐induced renal fibrosis is different from the clinical scenario, as complete acute obstruction can be promptly relieved through surgical intervention in patients.…”

Section: Discussionmentioning

confidence: 99%

“…The blunt dissection of the left ureter was followed by double ligation of the lower ureter. Next, the abdominal cavity was closed . Five days after the surgery, 24 of the rats had obstructed ureters reimplanted into their bladders.…”

Section: Methodsmentioning

confidence: 99%

Intravoxel Incoherent Motion and Arterial Spin Labeling MRI Analysis of Reversible Unilateral Ureteral Obstruction in Rats

Zhong

Wen

et al. 2018

Magnetic Resonance Imaging

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Background Renal fibrosis is a common consequence of chronic kidney disease (CKD) and is the mechanism by which various forms of CKD progress to endstage renal failure. Accurate assessment of renal fibrosis is important for treatment. Purpose To measure longitudinal changes of intravoxel incoherent motion (IVIM) and arterial spin labeling (ASL) before and after reversible unilateral ureteral obstruction in an animal model. Study Type Self‐controlled animal study. Animal Model Surgical obstruction of the ureters was performed and then removed after 5 days. Rats were scanned on Days 0, 1, 3, and 5 after creating the obstruction and on Days 4, 7, and 12 after releasing the obstruction. Field Strength/Sequence 3.0T/IVIM/ASL. Assessment The apparent diffusion coefficient (ADC), pure molecular diffusion (D), perfusion fraction (f), pseudodiffusion (D*), and renal blood flow (RBF) obtained from the ASL were measured. Statistical Tests Using SPSS v. 20.0 software, P < 0.05 were considered statistically significant. The data from each timepoint were compared using one‐way analysis of variance and correlation analysis was applied to various parameters. Results The postobstruction kidneys showed renal tubule swelling and increased collagen fiber content. Renal tubule swelling was relieved after reversing the obstruction, but Masson staining and cell density analysis revealed progressive changes that were primarily localized to the medulla. In general, ADC, D, f, D*, and RBF decreased with time during the 5 days of obstruction, and increased after release of the obstruction. ADC positively correlated with D, f, D*, and RBF (r = 0.415, r = 0.634, r = 0.465 r = 0.586, P < 0.001, respectively) in the cortex in this study. Also, ADC showed a positive correlation with D, f, and D* (r = 0.724, r = 0.749, r = 0.151, P < 0.001, respectively) in the medulla. Data Conclusion Kidney perfusion was the major factor affecting ADC. Functional imaging may be useful for following progression of CKD. Level of Evidence: 1 Technical Efficacy: Stage 2 J. Magn. Reson. Imaging 2019;50:288–296.

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“…An attractive characteristic compared with other imaging modalities is the ability to combine 1 H and 13 C MRI for multiple measures of both anatomy and function. For example, a comprehensive assessment of the kidney may also include renal fibrosis measurements using diffusion‐weighted MRI or T 1 mapping . Combined imaging of metabolism by hyperpolarized 13 C MRI with proton methods could yield important information about the possible interplay and timing between metabolic changes, inflammation and fibrosis that could help to guide the utility of functional MRI of the kidney as a means to monitor renal disease severity and progression …”

Section: Discussionmentioning

confidence: 99%

Evaluation of renal metabolic response to partial ureteral obstruction with hyperpolarized ¹³C MRI

et al. 2017

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Hyperpolarized C magnetic resonance imaging (MRI) may be used to non-invasively image the transport and chemical conversion of C-labeled compounds in vivo. In this study, we utilize hyperpolarized C MRI to evaluate metabolic markers in the kidneys longitudinally in a mouse model of partial unilateral ureteral obstruction (pUUO). Partial obstruction was surgically induced in the left ureter of nine adult mice, leaving the right ureter as a control. H and hyperpolarized [1- C]pyruvate MRI of the kidneys was performed 2 days prior to surgery (baseline) and at 3, 7 and 14 days post-surgery. Images were evaluated for changes in renal pelvis volume, pyruvate, lactate and the lactate to pyruvate ratio. After 14 days, mice were sacrificed and immunohistological staining of both kidneys for collagen fibrosis (picrosirius red) and macrophage infiltration (F4/80) was performed. Statistical analysis was performed using a linear mixed effects model. Significant kidney × time interaction effects were observed for both lactate and pyruvate, indicating that these markers changed differently between time points for the obstructed and unobstructed kidneys. Both kidneys showed an increase in the lactate to pyruvate ratio after obstruction, suggesting a shift towards glycolytic metabolism. These changes were accompanied by marked hydronephrosis, fibrosis and macrophage infiltration in the obstructed kidney, but not in the unobstructed kidney. Our results show that pUUO is associated with increased pyruvate to lactate metabolism in both kidneys, with injury and inflammation specific to the obstructed kidney. The work also demonstrates the feasibility of the use of hyperpolarized C MRI to study metabolism in renal disease.

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Use of intravoxel incoherent motion MRI to assess renal fibrosis in a rat model of unilateral ureteral obstruction

Abstract: IVIM MRI shows great potential in noninvasive assessment of renal fibrosis induced by UUO. J. Magn. Reson. Imaging 2016;44:698-706.

Cited by 37 publications

References 29 publications

Chronic kidney disease: Pathological and functional evaluation with intravoxel incoherent motion diffusion‐weighted imaging

Chronic kidney disease: Pathological and functional evaluation with intravoxel incoherent motion diffusion‐weighted imaging

Intravoxel Incoherent Motion and Arterial Spin Labeling MRI Analysis of Reversible Unilateral Ureteral Obstruction in Rats

Evaluation of renal metabolic response to partial ureteral obstruction with hyperpolarized ¹³C MRI

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Use of intravoxel incoherent motion MRI to assess renal fibrosis in a rat model of unilateral ureteral obstruction

Abstract: IVIM MRI shows great potential in noninvasive assessment of renal fibrosis induced by UUO. J. Magn. Reson. Imaging 2016;44:698-706.

Cited by 37 publications

References 29 publications

Chronic kidney disease: Pathological and functional evaluation with intravoxel incoherent motion diffusion‐weighted imaging

Chronic kidney disease: Pathological and functional evaluation with intravoxel incoherent motion diffusion‐weighted imaging

Intravoxel Incoherent Motion and Arterial Spin Labeling MRI Analysis of Reversible Unilateral Ureteral Obstruction in Rats

Evaluation of renal metabolic response to partial ureteral obstruction with hyperpolarized 13C MRI

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Evaluation of renal metabolic response to partial ureteral obstruction with hyperpolarized ¹³C MRI