Use of intestinal Pseudomonas aeruginosa in fish to detect the environmental pollutant benzo[a]pyrene

Karami, Ali; Christianus, Annie; Ishak, Zamri; Shamsuddin, Zulkifli; Masoumian, Majid; Courtenay, Simon C.

doi:10.1016/j.jhazmat.2012.02.038

Cited by 25 publications

(7 citation statements)

References 77 publications

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“…The molecular structure of PAHs has a major influence on their toxicity. Some members of this group are carcinogenic, mutagenic, and teratogenic and can produce cascades of adverse effects on aquatic organisms (Karami et al, 2012) and humans (Masiol et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Baseline distributions and sources of Polycyclic Aromatic Hydrocarbons (PAHs) in the surface sediments from the Prai and Malacca Rivers, Peninsular Malaysia

Keshavarzifard

Zakaria

Shau-Hwai

et al. 2014

Marine Pollution Bulletin

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In this study, the surface sediments of the Malacca and Prai Rivers were analyzed to identify the distributions, and sources of Polycyclic Aromatic Hydrocarbons (PAHs). The total PAH concentrations varied from 716 to 1210 and 1102 to 7938 ng g(-1)dw in the sediments of the Malacca and Prai Rivers, respectively. The PAH concentrations can be classified as moderate and high level of pollution in the sediments of the Malacca and Prai Rivers, respectively. The comparison of PAHs with the Sediment Quality Guidelines (SQGs) indicates that the PAHs in the sediments of the Malacca and Prai Rivers may have the potential to cause adverse toxicity effects on the sampled ecosystems. The diagnostic ratios of individual PAHs indicate both petrogenic- and pyrogenic-origin PAHs with dominance of pyrogenic source in both rivers. These findings demonstrate that the environmental regulations in Malaysia have effectively reduced the input of petrogenic petroleum hydrocarbons into rivers.

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Section: Introductionmentioning

confidence: 99%

Baseline distributions and sources of Polycyclic Aromatic Hydrocarbons (PAHs) in the surface sediments from the Prai and Malacca Rivers, Peninsular Malaysia

Keshavarzifard

Zakaria

Shau-Hwai

et al. 2014

Marine Pollution Bulletin

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“…Diols, dihydrodiols and tetradiols derived of PAH biotransformation are conjugated with glutathione via GST activity to be excreted through the bile (Oliva et al 2010, Fonseca et al 2011, Kammann et al 2014. Although it is not a completely suitable comparison, in C. gariepinus exposed intramuscularly to BaP, liver GST activity showed an inverse response with regard to the CFU concentration of PM4 strain suggesting that the hydrocarbon induced some physiological changes in the fish allowing altered intestinal bacterial populations (Karami et al 2012). Differences between GST activities of Pseudomonas strains with regard to hepatic GST in the fish could be explained by bile production favoring the excretion of glutathione-conjugated metabolites.…”

Section: Discussionmentioning

confidence: 99%

“…The number of hydrocarbon-degrading bacteria regarding the total heterotrophic bacteria in the digestive tract of molluscs and anostracan, are increased in more PAH-polluted localities with regard to a reference site (Šyvokienė and Mickėnienė 2004). Changes of intestinal P. aeruginosa in the juvenile African catfish (Clarias gariepinus) were related to intramuscular doses of benzo[a]pyrene (BaP), as well as some biomarkers in the liver of the fish (ethoxyresorufin-O-deethylase and glutathione S-transferase) were inversely correlated with the bacterial population (Karami et al 2012). Despite these studies about the capacity of some bacteria to degrade PAHs, a lack of information prevails about the possible protective role of Pseudomonas spp.…”

Section: Introductionmentioning

confidence: 99%

TOXICOKINETIC AND TOXICODYNAMIC SYMBIOTIC INTERACTIONS AMONG INTESTINAL Pseudomonas DEGRADING OF HYDROCARBONS WITH ITS WILD HOST FISH Chirostoma jordani

Rangel

Madera-Sandoval

Castro-Escarpulli

et al. 2018

Rev. Int. Contam. Ambie.

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The symbiotic relationship between native microbiota and their hosts probably is a key factor in animal survival. In this study, the relationships of Pseudomonas spp. strains with specific biomarkers of exposure to polycyclic aromatic hydrocarbons (PAHs) in wild fish Chirostoma jordani, as well as the capacity of these bacteria to biotransform PAHs were evaluated. The activity of the naphthalene dioxygenase system of the bacteria exposed to 0.1, 1.0 and 10 µg/L of PAHs was higher than the mean isoform 1A1 (CYP1A1) of the wild fish, particularly bacterial species related to Pseudomonas spp. However, the epoxide hydrolase activity of the strains was lower in all cases compared to the fish. Glutathione S-transferase (GST) activity of the bacterial strains was lower than in the liver, but higher than in viscera of C. jordani. Using redundancy analysis, two differential patterns were found: (i) CYP1A1 activity of fish was induced by naphthalene and anthracene water levels independently of sampling season and lakes monitored, and (ii) the unidentified strain of the Pseudomonas genus biotransforms the endogenous levels of benzo[a]pyrene, benzo[k]fluoranthene and indeno[1,2,3-cd]pyrene in the fish allowing the hydroxylated metabolites to conjugate with glutathione through GST activity of the fish. Palabras clave: CYP 1A1, epóxido hidrolasa, GST, HAP, oxidasas, sistema NDO RESUMEN Las relaciones simbióticas entre la microbiota nativa y sus hospederos probablemente son un factor clave en la supervivencia animal. En el presente estudio se evaluaron las relaciones de cepas de Pseudomonas spp. con biomarcadores específicos de exposición a hidrocarburos aromáticos policíclicos (HAP) en Chirostoma jordani silvestre, así como la capacidad de estas bacterias para biotransformar HAP. La actividad del sistema de naftalen-dioxigenasa de las bacterias expuestas a 0,1, 1,0 y 10 μg/L de HAP fue superior al promedio de la isoforma 1A1 (CYP1A1) en los peces, particularmente cepas relacionadas con especies de Pseudomonas spp. Sin embargo, la actividad de la epóxido hidrolasa de las bacterias fue menor en comparación con los peces. La catálisis de la glutatión-S-transferasa (GST) de las cepas bacterianas fue menor que en

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“…Polycyclic aromatic hydrocarbons (PAHs) are carcinogenic, mutagenic and toxic to the marine environment and to the human. Their emergence to the environment has become a serious concern to the stake-holders and researchers (Cao et al 2009, Karami et al 2012. The sources of PAHs are broadly categorized into three types: pyrogenic-derived from the incomplete combustion; petrogenic-caused by the petroleum sources; and diagenic-derived from plant materials and precursors (Zakaria et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Formation and weathering assessment of oil-suspended sediment aggregates through a laboratory investigation

Suneel

Kumar

Balaji

et al. 2021

Environ Sci Pollut Res

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Formation of oil-suspended sediment aggregates (OSAs) is believed to be one of the natural cleaning processes in the marine environment. In this study, we have investigated the formation processes of OSAs under different mixing periods (continuous mixing and with the addition of sediments in between), oil-sediment ratios (1:1, 1:2 and 2:1) and crude oils (Arabian Light (AL), Kuwait (KW) and Murban (MB)). The results revealed that size of OSAs significantly increased (up to ≈ 1.41 mm) with the addition of sediments. Aggregates (total 36) were extracted for n-alkanes and polycyclic aromatic hydrocarbons to quantify and assess their weathering and toxic levels. The maximum n-alkane depletion was 84% (111-02), 94% (212-02) and 84% (321-02) and PAH depletion was ≈ 72% (111-02), 79% (212-02) and 81% (311-03) for the OSAs of AL, KW and MB crude oils, respectively, for the different samples considered, indicating that n-alkanes were depleted relatively higher than the PAHs. The highest depletion of both n-alkane and PAHs has occurred in OSAs of 10-h continuous mixing. The depletion of both n-alkane and PAHs reduced after the addition of sediments, however, escalated the growth of OSAs, resulting in bigger size OSAs. The concentration of PAHs of all 36 OSAs is greater than 5000 ng/g, indicating very high PAH pollution. Though the formation of OSAs helps in cleaning the spill sites, the carcinogenic threat to the marine ecosystem caused by these OSAs cannot be ignored.

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Use of intestinal Pseudomonas aeruginosa in fish to detect the environmental pollutant benzo[a]pyrene

Cited by 25 publications

References 77 publications

Baseline distributions and sources of Polycyclic Aromatic Hydrocarbons (PAHs) in the surface sediments from the Prai and Malacca Rivers, Peninsular Malaysia

Baseline distributions and sources of Polycyclic Aromatic Hydrocarbons (PAHs) in the surface sediments from the Prai and Malacca Rivers, Peninsular Malaysia

TOXICOKINETIC AND TOXICODYNAMIC SYMBIOTIC INTERACTIONS AMONG INTESTINAL Pseudomonas DEGRADING OF HYDROCARBONS WITH ITS WILD HOST FISH Chirostoma jordani

Formation and weathering assessment of oil-suspended sediment aggregates through a laboratory investigation

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