Use of indirect lymphography to identify sentinel lymph node in dogs: a pilot study in 30 tumours

Brissot, Hervé; Edery, Elsa

doi:10.1111/vco.12214

Cited by 60 publications

(97 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Last, the regional LN does not necessarily represent the sentinel LN, which is by definition the first node that receives direct lymphatic drainage from the tumour rather than the closest node to the primary tumour. 26,27 Different methods of identification of the sentinel LN have been used, including radioisotope injection, vital blue dye, or lymphangiography. For LNs not obviously metastatic, sentinel LNs techniques rather than anatomic sampling should be applied to accurately reflect the metastatic status.…”

Section: Discussionmentioning

confidence: 99%

Therapeutic impact of regional lymphadenectomy in canine stage II cutaneous mast cell tumours

Marconato

Polton

Stefanello

et al. 2018

Vet Comparative Oncology

111

View full text Add to dashboard Cite

Lymph node (LN) metastasis in canine cutaneous mast cell tumours (cMCTs) is a well-known negative prognostic factor. The role of lymphadenectomy in the treatment of stage II disease remains controversial because of its uncertain therapeutic benefit. Aim of this retrospective study was to investigate the impact of lymphadenectomy on tumour control and survival for dogs with stage II cMCTs. Dogs with firstly occurring, histologically confirmed cMCT with LN metastasis undergoing resection of the primary tumour and medical treatment thereafter were retrospectively enrolled. Dogs were classified into two groups: LN sampling (LNS; diagnosis of metastasis obtained by cytology) and regional LN dissection (LND; diagnosis obtained by histopathology). To determine the therapeutic value of lymphadenectomy, the characteristics of recurrence (local, nodal and distant) and survival were compared between groups. Evaluated outcome variables included signalment, anatomic location, diameter, ulceration, substage, surgical margins, Patnaik grading, Kiupel grading and medical treatment. Overall, 152 dogs were included: 81 underwent LND as part of primary surgery and 71 LNS. The median follow-up time was 409 days for LND group and 620 days for LNS group. On univariable analysis, the risk of developing local, nodal or distant relapse was significantly higher in the LNS group compared with LND (P < 0.001). On multivariable analysis, the risk of tumour progression and tumour-related death were 5.47 and 3.61 times higher in the LNS group, respectively (P < 0.001). Regional lymphadenectomy may have therapeutic value and improve prognosis in dogs with stage II cMCTs undergoing surgical removal of the primary tumour and medical treatment.

show abstract

Section: Discussionmentioning

confidence: 99%

Therapeutic impact of regional lymphadenectomy in canine stage II cutaneous mast cell tumours

Marconato

Polton

Stefanello

et al. 2018

Vet Comparative Oncology

111

View full text Add to dashboard Cite

show abstract

“…Invasive techniques such as elective neck dissection or sentinel node biopsy with subsequent histopathology and step sectioning remain the standard of care for humans . Both elective dissection and sentinel lymph node techniques have been reported in dogs; however, their therapeutic and diagnostic value have not been directly assessed . Until other techniques are validated as sufficiently sensitive alternatives, lymph node excision and histopathology (whether sentinel or regional) remains the standard of care for lymph node staging in dogs, not least due its ability to identify small metastatic aggregates.…”

Section: Discussionmentioning

confidence: 99%

“…[39][40][41][42][43] Both elective dissection and sentinel lymph node techniques have been reported in dogs; however, their therapeutic and diagnostic value have not been directly assessed. 12,32,[44][45][46] Until other techniques are validated as sufficiently sensitive alternatives, lymph node excision and histopathology (whether sentinel or regional) remains the standard of care for lymph node staging in dogs, not least due its ability to identify small metastatic aggregates. Half of the dogs identified to have lymph node metastasis were found to have micrometastasis or isolated tumour cells.…”

Section: Discussionmentioning

confidence: 99%

Diagnostic accuracy of contrast‐enhanced computed tomography for assessment of mandibular and medial retropharyngeal lymph node metastasis in dogs with oral and nasal cancer

Skinner

Boston

Giglio

et al. 2018

Vet Comparative Oncology

View full text Add to dashboard Cite

The diagnostic accuracy of contrast-enhanced CT for detection of cervical lymph node metastasis in dogs is unknown. The purpose of this retrospective, observational, diagnostic accuracy study was to assess the efficacy of CT for detection of mandibular and medial retropharyngeal lymph node metastasis in dogs. Histopathology of dogs with cancer of the head, CT and bilateral mandibular and medial retropharyngeal lymphadenectomy was reviewed. A single radiologist measured lymph nodes to derive short axis width and long-short axis ratios. Two blinded radiologists separately assessed lymph node margins, attenuation and contrast enhancement and each provided a final subjective interpretation of each node site as benign or neoplastic. Where radiologists' opinions differed, a consensus was reached. Sensitivity, specificity and accuracy were calculated for mandibular and medial retropharyngeal sites. Agreement between radiologists was assessed. Fisher's exact test and the Kruskal-Wallis H-test were used to assess associations between variables. Forty-one primary tumours were recorded in 40 dogs. Metastasis to mandibular or retropharyngeal lymph nodes occurred in 16 out of 40 dogs (43/160 nodes). Agreement between radiologists was almost perfect for margination, attenuation and enhancement, strong for interpretation of mandibular lymph node metastasis, and weak for interpretation of medial retropharyngeal lymph node metastasis. Sensitivity of CT was 12.5% and 10.5%, specificity was 91.1% and 96.7%, and accuracy was 67.5% and 76.3% for mandibular and medial retropharyngeal lymph nodes respectively. No individual CT findings were predictive of nodal metastasis. Given the low sensitivity of CT, this modality cannot be relied upon alone for assessment of cervical lymph node metastasis in dogs.

show abstract

“…The use of noninvasive sentinel lymph node mapping techniques were strongly advocated in veterinary medicine, and in the last few years some studies combining lymphoscintigraphy and peritumoral injection of methylene blue dye have been published . Furthermore, contrast agent‐related techniques for lymph node identification useful in a clinical oncologic setting have been prior described experimentally and clinically in animals, including indirect lymphography and contrast ultrasound …”

Section: Introductionmentioning

confidence: 99%

Computed tomographic‐lymphography as a complementary technique for lymph node staging in dogs with malignant tumors of various sites

Rossi

Körner

Suarez

et al. 2017

Vet Radiology Ultrasound

112

View full text Add to dashboard Cite

Locoregional lymph nodes are routinely examined in order to define the spatial extent of neoplastic disease. As draining patterns of certain tumor types can be divergent from expected anatomical distribution, it is critical to sample the lymph nodes truly representing the draining area. The aim of this bicenter prospective pilot study was to describe the technique of computed tomographic (CT)-lymphography for primary draining lymph node mapping in tumor staging in dogs. Forty-five dogs with macro- or microscopic tumors in specified localizations were evaluated. Depending on body weight, 0.8-2 ml contrast agent (iohexol) was injected into four quadrants around the tumor, and CT-images were obtained at 1, 3, 6, 9, and 12 minutes post-injection. Attenuation of chosen regions of interest (Hounsfield units (HU)) and patterns of enhancement were assessed for 284 lymph nodes in the precontrast study with median HUs of 31.1 (Interquartile range (IQR) = 18.4) and for 275 in the intravenous postcontrast study with 104.3 HU (IQR = 31.2) (paired Wilcoxon test, P < 0.001). In the CT-lymphography study, 45 primary draining lymph nodes with a significantly higher median HU value of 348.5 (IQR = 591.4) (one-sample t-test, P < 0.001) were identified. Primary draining lymph nodes were found to be clearly visible after 1-3 minutes after local injection, often concurrent with a good visibility of the lymphatic vessel system. The herein described technique of peritumorally injected CT-contrast agent followed by subsequent CT-lymphography for primary draining lymph node mapping works well in a majority of cases in all investigated sites and warrants further validation for different tumor entities.

show abstract

Use of indirect lymphography to identify sentinel lymph node in dogs: a pilot study in 30 tumours

Cited by 60 publications

References 51 publications

Therapeutic impact of regional lymphadenectomy in canine stage II cutaneous mast cell tumours

Therapeutic impact of regional lymphadenectomy in canine stage II cutaneous mast cell tumours

Diagnostic accuracy of contrast‐enhanced computed tomography for assessment of mandibular and medial retropharyngeal lymph node metastasis in dogs with oral and nasal cancer

Computed tomographic‐lymphography as a complementary technique for lymph node staging in dogs with malignant tumors of various sites

Contact Info

Product

Resources

About