Use of in vitro electroporation and slice culture for gene function analysis in the mouse embryonic spinal cord

Li, Shuanqing; Li, Yunxiao; Han, Lei; Yang, Ciqing; Lin, Juntang

doi:10.1016/j.mod.2019.103558

Cited by 3 publications

(2 citation statements)

References 18 publications

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“…The axonal projections are unanimously directed from inside to the lateral side of the spinal cord. In comparison to neurons present in vivo, a single-neuron growing in the slice culture owns an extra number of complete neurites and therefore offers ease in the study of structural and behavioral alterations in individual neurons [159].…”

Section: Electroporationmentioning

confidence: 99%

A Single-Neuron: Current Trends and Future Prospects

Gupta

Balasubramaniam

Chang

et al. 2020

Cells

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The brain is an intricate network with complex organizational principles facilitating a concerted communication between single-neurons, distinct neuron populations, and remote brain areas. The communication, technically referred to as connectivity, between single-neurons, is the center of many investigations aimed at elucidating pathophysiology, anatomical differences, and structural and functional features. In comparison with bulk analysis, single-neuron analysis can provide precise information about neurons or even sub-neuron level electrophysiology, anatomical differences, pathophysiology, structural and functional features, in addition to their communications with other neurons, and can promote essential information to understand the brain and its activity. This review highlights various single-neuron models and their behaviors, followed by different analysis methods. Again, to elucidate cellular dynamics in terms of electrophysiology at the single-neuron level, we emphasize in detail the role of single-neuron mapping and electrophysiological recording. We also elaborate on the recent development of single-neuron isolation, manipulation, and therapeutic progress using advanced micro/nanofluidic devices, as well as microinjection, electroporation, microelectrode array, optical transfection, optogenetic techniques. Further, the development in the field of artificial intelligence in relation to single-neurons is highlighted. The review concludes with between limitations and future prospects of single-neuron analyses.

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Section: Electroporationmentioning

confidence: 99%

A Single-Neuron: Current Trends and Future Prospects

Gupta

Balasubramaniam

Chang

et al. 2020

Cells

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“…З кінця XIX сторіччя до сьогодення накопичилось чимало наукової інформації щодо досліджень морфології спинного мозку в онтогенезі людини, починаючи з макроанатомічних та закінчуючи імуногістохімічними, імунофлуоресцентними та УЗД і КТ-дослідженнями [1]. Завдяки розвитку та удосконаленню медичних технологій, методів діагностики в останні роки зросла зацікавленість нейроморфологів, невропатологів, нейрохірургів та спеціалістів репродуктології до гістогенезу структур центральної нервової системи (ЦНС), зокрема, спинного мозку [2][3][4].…”

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Fetal Anatomy of the Human Spinal Cord

Школьніков¹

2020

CAOS

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Due to the development and improvement of medical technologies and diagnostic methods, in recent years, the interest of neuromorphologists, neuropathologists, neurosurgeons and reproductive specialists in the histogenesis of the structures of the central nervous system, in particular, the spinal cord, has increased. In the process of macro- and microscopic examination of the spinal cord of human fetuses of 20-21 weeks of intrauterine development, the topography of the thickenings in relation to the parts of the spinal column was established according to our own method, the morphometric parameters of the structures of the spinal cord segments and the regularities of cytoarchitectonics were determined. In 20-21 week old fetuses, the ratio of the length of the spine to the parietococcygeal length of the fetus is 65.0%, and the ratio of the length of the spinal cord to the parietococcygeal length of the fetus is 54.0 %. The border between the cervical and thoracic spine is projected onto a conditional line that connects the spine of the scapula. The border between the thoracic and lumbar regions of the spine is the line between the upper three quarters and the lower one quarter of the body length. The border between the lumbar and sacral parts runs along a conventionally drawn line that connects the posterior lower iliac spines, and the border of the transition of the sacral to the coccygeal is the level of the lower third of the gluteal region. The structure of the gray matter of the spinal cord segments in this age period corresponds to that in people of mature age – the presence of anterior, lateral and posterior horns. A large area of gray matter is observed in the cervical and lumbar segments, a smaller area in the thoracic and sacral segments. The structuredness of the white matter of the spinal cord segments in this age period corresponds to that in adults – the presence of anterior, lateral and posterior cords. The cervical and lumbar segments have a large area of white matter, and in magnitude they are the same. The nuclei of radial glial cells are relatively equal in size in all segments. The thickness of the matrix layer varies throughout the entire spinal cord, but reaches its greatest size in the ventral parts. The sizes of the nuclei of neuroblasts also fluctuate: the nuclei of motor neurons have large sizes, and the smaller ones are inserted and vegetative. The nuclei of glial cells have relatively identical sizes of different segments of the spinal cord, but 2-3 times less than the nuclei of neuroblasts.

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Modelling of reversible tissue electroporation and its thermal effects in drug delivery

Mondal

Dalal

2023

European Journal of Pharmaceutics and Biopharmaceutics

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Use of in vitro electroporation and slice culture for gene function analysis in the mouse embryonic spinal cord

Cited by 3 publications

References 18 publications

A Single-Neuron: Current Trends and Future Prospects

A Single-Neuron: Current Trends and Future Prospects

Fetal Anatomy of the Human Spinal Cord

Modelling of reversible tissue electroporation and its thermal effects in drug delivery

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