Use of image analysis to assess the plasma membrane integrity of ram spermatozoa in different diluents

Toward an integrative and predictive sperm quality analysis in Bos taurus

“…The finding that sperm viability was positively correlated with the acrosomal integrity was also expected, as observation of the images revealed the presence of more spermatozoa with acrosomal loss in the dead than in the live sperm populations. Finally, sperm membrane integrity was correlated with sperm motility, in agreement with previous studies (Jeyendran et al, 1984;Fraser et al, 2001;Mantovani et al, 2002;Kirk et al, 2005;Yaniz et al, 2008b;2011). This association may be explained by the fact that all spermatozoa with damaged plasma membrane were static.…”

Management Factors Affecting Fertility in Sheep

“…For this reason, laboratory assessment of semen must include the testing of as many relevant sperm attributes for fertilisation and embryo development as possible, not only in individual spermatozoa but also within a large sperm population (Rodríguez-Martínez, 2003). In practice, routine sperm analysis requires fast, objective and accessible methods of assessing different aspects of sperm viability (Yániz et al, 2008). In this sense, the common use of computer-assisted sperm analysis (CASA) methods for sperm motility, of image analysis for the evaluation of membrane integrity (Yániz et al, 2008), of DNA fragmentation with sperm chromatin diffusion techniques (SCD), (Gosalvez et al, 2008;López-Fernández et al, 2008), or fluorimetry, etc., would theoretically improve the predictive capacity of semen analysis, although more studies are needed to determine the utility of these techniques in the practical use of AI.…”

“…For example, in a recent work (Yániz et al, 2011) we studied the effect of different buffer systems included in the composition of well-defined and synthetic diluents (in vitro), on sperm quality parameters during storage at 15ºC. TRIS caused drastic modifications of certain sperm kinematic parameters during storage at 15ºC although, along with citrate, it is one of the main buffers included in the composition of semi-synthetic ram semen diluents (López et al,1999;López-Sáez et al, 2000;Salamon and Maxwell, 2000;Paulenz et al, 2003;Martí et al, 2003;Yániz et al, 2008). With the available diluents, semen preservation in the non-frozen state is limited to 6-8h without reducing fertility (Maxwell and Salamon (1993); Salamon and Maxwell, 2000).…”

Effect of argan oil on liquid storage of ram semen in Tris or skim milk based extenders

“…One of the critical steps of AI is the ram semen preservation, liquid 48 storage or freezing. The freezing-thawing of ram semen results in stronger damages to spermatozoa 49 when compared to liquid storage (Evans, 1987).Therefore, intrauterine insemination may be needed to The percentages of total and progressive motile spermatozoa in each sample were determined 143 using a computer-assisted sperm analysis system (ISAS, version 1.0.17, Proiser, Valencia, Spain) as 144 described by Yániz et al (2008). A spermatozoa was defined as non-motile if average path velocity 145 (VAP) was lower than 10 µm/s and sperm cells were considered as progressive motile when VAP was 146 higher than 75 µm/s and straightness index (STR) was higher than 80%.…”

“…Yániz et al (2008). A spermatozoa was defined as non-motile if average path velocity 145 (VAP) was lower than 10 µm/s and sperm cells were considered as progressive motile when VAP was146 higher than 75 µm/s and straightness index (STR) was higher than 80%.…”