Use of image analysis techniques to determine the embryo size of Senna multijuga (Rich.) seeds and its relation to germination and vigor

Marchi, José Luís de; Gomes-Júnior, Francisco Guilhien

doi:10.1590/2317-1545v39n1165423

Cited by 12 publications

(13 citation statements)

References 18 publications

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“…The morphometric variables showed no difference between lots although they have been constantly associated with the germinative performance of forest seeds. Marchi and Gomes-Junior (2017) observed a relationship between the area of seeds of Senna multijuga and its physiological potential. However, the authors emphasize that several aspects must be considered, such as the internal cavity occupied by the embryo and endosperm, the presence of mechanical damage, and spots indicating tissue deterioration or seed malformations, which can influence germination.…”

Section: Resultsmentioning

confidence: 94%

“…The use of the X-ray technique was satisfactory to identify these characteristics in a non-destructive, precise, and fast way. Other works have also successfully used the technique for analyzing seeds of forest species such as Tabebuia heptaphylla (Amaral et al, 2011), Acca sellowiana (Silva et al, 2013), Terminalia argentea (Gomes et al, 2014), Platypodium elegans (Gomes et al, 2016), Senna multijuga (Marchi and Gomes-Junior, 2017), Moringa oleifera (Noronha et al, 2018), Leucaena leucocephala (Medeiros et al, 2018), Lecythis pisonis (Rosa et al, 2020), Arctostaphylos pungens, Juniperus deppeana (Rubalcava-Castillo et al, 2020), and Pinus sibirica (Arkhipov et al, 2020).…”

Section: Resultsmentioning

confidence: 99%

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Seed quality analysis of Senna siamea Lam. using image analysis techniques

et al. 2020

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The inclusion of image analysis techniques for the accurate and rapid assessment of the quality of forest seeds is highly desirable. The use of digital radiographic images of seeds and the analysis of seedling images, still little used in determining the quality of forest seeds, are among the available imaging techniques. This study aimed to assess the feasibility of using the analysis of images of seeds and seedlings in the assessment of the physical and physiological quality of Senna siamea seeds. Radiographic images of seeds were obtained from five seed lots, allowing generating morphological and tissue integrity descriptors. These seeds were then subjected to germination and seedling growth tests, which allowed obtaining variables related to physiological quality. The generated seedlings were scanned and analyzed using the software ImageJ. The data were analyzed using analysis of variance, correlation, and principal component analysis. The results showed differences between seed lots in terms of physiological quality and physical integrity of internal tissues. Significant correlations were observed between the variables obtained with the radiographic analysis and seed physiological characterization tests. The use of techniques to analyze seed radiographs and seedling images allows access to information on the physical and physiological integrity of S. siamea seeds.

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Section: Resultsmentioning

confidence: 94%

Section: Resultsmentioning

confidence: 99%

Seed quality analysis of Senna siamea Lam. using image analysis techniques

et al. 2020

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“…According to Abud et al (2018), studying broccoli seeds, the area of the seeds may reflect their vigor, since larger areas coincide with larger reserves available for seed germination and seedling growth. Marchi and Gomes Junior (2017) demonstrated that, for Senna multijuga seeds, it was the embryo area that most clearly expressed differences in the physiological quality of the lots in relation to the pericarp area. Likewise, larger values for embryo area were observed in lots 1, 6, 7, 8 and 10, indicative of the higher physiological quality of the seeds from these lots.…”

Section: Resultsmentioning

confidence: 99%

Use of radiographic images for rapid and non-destructive assessment of crambe seed quality

et al. 2021

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Technologies based on electromagnetic radiation, such as the X-ray technique, has contributed to the establishment of new and promising methodologies for evaluating seed quality. This study aimed to relate parameters based on semi-automated analysis of radiographs of crambe seeds to their physiological quality. Radiographic images of seeds from 10 seed lots of cultivar FMS Brilhante were semi-automatically analyzed using ImageJ® software. Measurements of morphometric characteristics and tissue integrity were obtained for the seeds, as well as individually for the seed embryo. Following X-ray test, the seeds were subject to germination and seedling growth test. It was possible to visualize the internal structures of the seeds in the radiographs. There were differences in the physical parameters obtained by the semi-automated analysis of the radiographs between the seed lots. Also, the lots differed regarding the physiological quality of the seeds. Morphometric characteristics and tissue integrity, especially for the seed embryo, showed high correlation with the seed physiological quality. Therefore, this work presents an efficient approach to rapid and non-destructively assess the quality of crambe seeds.

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“…In regard to seeds, the use of non-destructive analyses, such as the X-ray test, has been documented as eff ective for studies of internal morphology of seeds of diverse species, such as Moquiniastrum polymorphum (Faria et al, 2019), Moringa oleifera (Noronha et al, 2018), and Leucaena leucocephala (Medeiros et al, 2018), among others. This technique assists in identifi cation of full seeds, empty seeds, mechanical damage, and spots that indicate deterioration, characteristics that can aff ect seed physiological potential (Marchi and Gomes Junior, 2017), and, consequently, the quality of seed lots.…”

Section: Introductionmentioning

confidence: 99%

GERMINATION AND POST-SEMINAL DEVELOPMENT OF Melaleuca alternifolia (MAIDEN & BETCHE) CHEEL

et al. 2020

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There is little information regarding the germination pattern, seed characterization, and seedling development of Melaleuca alternifolia. This study aimed to determine the ideal temperature for the germination of M. alternifolia seeds, as well as to characterize the seeds and the post-seminal development of the species. Three lots of M. alternifolia seeds were placed to germinate at 20, 25, 20-30, 20-35, 30, and 35 °C, with daily evaluations to determine germination rate, germination speed index (GSI), speed of germination (SG), and mean time for germination of 50% (t50) and 100% (t100) of seeds. The inner morphology of the seeds was evaluated through X-ray images and seedling morphology by photographs. Alternating temperatures (20-30 and 20-35 °C) led to germination rates higher than the other temperatures. The GSI had the lowest values at the constant temperature of 20 °C and the highest values at the constant 30 °C for all the lots. The SG was lowest at a temperature of 20 °C and the highest at the temperature of 35 °C. At alternating temperatures (20-30 and 20-35 °C), t50 was around 5 days and t100 reached 16 days. In conclusion, the alternating temperatures of 20-30 °C and 20-35 °C are recommended for germination of M. alternifolia, and final evaluation can be performed at 16 days after sowing. The inner structures of seeds could be visualized by X-ray analysis, and full seeds could be distinguished from empty seeds and impurities. Seeds exhibit epigeal germination and seedling development is stabilized after 30 days.

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Use of image analysis techniques to determine the embryo size of Senna multijuga (Rich.) seeds and its relation to germination and vigor

Cited by 12 publications

References 18 publications

Seed quality analysis of Senna siamea Lam. using image analysis techniques

Seed quality analysis of Senna siamea Lam. using image analysis techniques

Use of radiographic images for rapid and non-destructive assessment of crambe seed quality

GERMINATION AND POST-SEMINAL DEVELOPMENT OF Melaleuca alternifolia (MAIDEN & BETCHE) CHEEL

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