Use of IL-18 production in a human keratinocyte cell line to discriminate contact sensitizers from irritants and low molecular weight respiratory allergens

Corsini, Emanuela; Mitjans, Montserrat; Galbiati, Valentina; Lucchi, Laura; Galli, C. L.; Marinovich, Marina

doi:10.1016/j.tiv.2009.04.005

Cited by 117 publications

(71 citation statements)

References 24 publications

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“…Evidence has been presented for the involvement of NOD-like receptors (NLR) as well as IL-1b, IL-18 and caspase-1 in the mouse CHS model [25,26]. Recent work has also suggested that IL-18 plays an important role by distinguishing the presence of contact allergens from irritants [27] (Table 1). The outcome of skin immune responses with respect to tolerance or immunity is dependent on skin NLRP3 inflammasome activation, and secreted IL-1b and IL-18 may regulate the quality of an allergen-specific T-cell response in CHS [25].…”

Section: Allergic Contact Hypersensitivitymentioning

confidence: 99%

The NLRP3 inflammasome, a target for therapy in diverse disease states

Cook¹,

Savic

Wittmann

et al. 2010

Eur J Immunol

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A role for NLRP3 inflammasome in recurrent and chronic inflammation was initially described in a group of rare autoinflammatory conditions, termed cryopyrin‐associated periodic syndrome. Subsequently, inflammasomes have been implicated in the pathology of many common diseases, including cancer, gout and diabetes. Despite diverse pathologies, the central role of the inflammasome in innate defences and tumour elimination suggests common therapeutic approaches to reduce inflammation where appropriate.

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Section: Allergic Contact Hypersensitivitymentioning

confidence: 99%

The NLRP3 inflammasome, a target for therapy in diverse disease states

Cook¹,

Savic

Wittmann

et al. 2010

Eur J Immunol

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“…Other types of assays are in development and include peptide reactivity and single cellbased assays using, for example, human keratinocytes and dendritic cells (DC). They assess the activation of the innate immune system and of the antioxidant response which are hallmarks of the reactivity of such chemicals [16][17][18][19][20][21][22][23]. The innate immune response is essential for both the sensitization to the chemical and the subsequent adaptive T-cellmediated immune response upon further contacts [18][19][20].…”

Section: Replacement Of Animal Testing: T Cells As Tools In Immunotoxmentioning

confidence: 99%

“…The innate immune response is essential for both the sensitization to the chemical and the subsequent adaptive T-cellmediated immune response upon further contacts [18][19][20]. In the single cell-based assays the sensitization potential of chemicals is tested by measuring, for example, chemicalinduced antioxidant responses [21,22] or IL-18 production in keratinocytes [23] or the induction of activation markers such as CD86 on the cell surface of DC [16,17].…”

Section: Replacement Of Animal Testing: T Cells As Tools In Immunotoxmentioning

confidence: 99%

T-cell recognition of chemicals, protein allergens and drugs: towards the development of in vitro assays

Martin

Esser

Schmucker

et al. 2010

Cell. Mol. Life Sci.

130

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Chemicals can elicit T-cell-mediated diseases such as allergic contact dermatitis and adverse drug reactions. Therefore, testing of chemicals, drugs and protein allergens for hazard identification and risk assessment is essential in regulatory toxicology. The seventh amendment of the EU Cosmetics Directive now prohibits the testing of cosmetic ingredients in mice, guinea pigs and other animal species to assess their sensitizing potential. In addition, the EU Chemicals Directive REACh requires the retesting of more than 30,000 chemicals for different toxicological endpoints, including sensitization, requiring vast numbers of animals. Therefore, alternative methods are urgently needed to eventually replace animal testing. Here, we summarize the outcome of an expert meeting in Rome on 7 November 2009 on the development of T-cell-based in vitro assays as tools in immunotoxicology to identify hazardous chemicals and drugs. In addition, we provide an This article is dedicated to the memory of our colleague and friend Reinhard Wanner, died 2 April 2010. Cellular and Molecular Life Sciences overview of the development of the field over the last two decades.

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“…In addition, after being targeted by IFN-, keratinocytes upregulate costimulatory molecules such as CD80 and are able to function as antigen presenting cells and facilitating activation of hapten specific T cells (Gaspari, 1997;Albanesi, 2010). Hence, keratinocytes are important in the sensitization and elicitation phase of ACD and for that reason these cells are often used for the development of in vitro assays for skin sensitization testing (Van Och et al, 2005;Corsini et al, 2009;Galbiati et al, 2011).…”

Section: Introductionmentioning

confidence: 99%