Use of <i>in vitro</i> release of interferon‐γ in the diagnosis of contact allergy to potassium dichromate – a controlled study

Trattner, Akiva; Akerman, Lehavit; Lapidoth, Moshe; Klein, Tirza; Weiss, Harry A.; Chaim, Berta Ben; David, M. Ojcius

doi:10.1034/j.1600-0536.2003.00067.x

Cited by 12 publications

(16 citation statements)

References 13 publications

(10 reference statements)

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“…Moreover, release of cytokines could only be detected in half of the MCI‐allergic patients. Regarding the metal chromate, lymphocyte proliferation responses could be detected in approximately 70% of chromate‐allergic patients (14–16). For fragrances, Sieben et al (17) found no or only very infrequent fragrance‐specific T‐cell responses, with only 31% of patch test‐positive patients having a positive T‐cell response.…”

Section: Introductionmentioning

confidence: 99%

Improved detection of allergen‐specific T‐cell responses in allergic contact dermatitis through the addition of ‘cytokine cocktails’

Moed

Blomberg

Bruynzeel

et al. 2005

Experimental Dermatology

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The gold standard for the diagnosis of allergic hypersensitivity is skin patch testing with the suspected allergens. This diagnostic tool, however, has distinct disadvantages, and therefore the development of alternative or complementary in vitro tests is of great importance. In this study, we evaluate the applicability of an in vitro test method, as developed earlier for nickel allergy, to detect allergen-specific T cells in the blood of patients allergic to frequent sensitizers (chromate, cobalt, paraphenylenediamine, fragrances and chloromethyl-isothiazolinone). Peripheral blood mononuclear cells (PBMCs) of allergic patients and healthy controls were cultured in the absence or presence of allergen. Additionally, type 1 (IL-7 and IL-12) or type 2 (IL-7 and IL-4) stimulating cytokines were added; after 6-day proliferation, IFN-gamma and IL-5 secretions were determined. Without the addition of cytokines, consistent allergen-induced proliferation was observed in PBMCs of nickel-allergic patients only. By contrast, the addition of type 1 or type 2 stimulating cytokines resulted in a significantly enhanced allergen-specific proliferation for all allergens tested (sensitivity increased from 26 to 43% or 38%, respectively, P < 0.05). In these cultures, allergen-induced IFN-gamma and IL-5 secretion was also significantly increased, compared to healthy controls (P < 0.05, for IFN-gamma sensitivity 79%, specificity 93%; for IL-5 sensitivity 74%, specificity 81%). In conclusion, these results demonstrate an increased proliferative capacity and cytokine production by allergen-specific T cells from allergic patients, but not of healthy individuals upon stimulation with allergens in combination with type 1 or 2 skewing cytokines. The present data warrant further exploration of the application of this test to a broader set of allergens.

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Section: Introductionmentioning

confidence: 99%

Improved detection of allergen‐specific T‐cell responses in allergic contact dermatitis through the addition of ‘cytokine cocktails’

Moed

Blomberg

Bruynzeel

et al. 2005

Experimental Dermatology

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“…Van den Broeke et al [19] reported a Th1 cytokine profile in patients allergic to nickel, and a previous study by our group of IFN-γ expression in peripheral blood mononuclear cells (PBMCs) of 20 patients with patch-proven allergy to potassium dichromate yielded significantly higher levels in the patients than in controls [8] . However, later studies suggested a role for Th2 cytokines.…”

Section: Discussionmentioning

confidence: 99%

“…Lymphocyte transformation and macrophage migration inhibition have been evaluated as measures of ACD in animals and humans [6,7] but have not yet gained widespread acceptance in clinical practice. Previously, using an ELI-SA technique, we demonstrated that lymphocytes from peripheral blood of individuals with chromate allergy produced significantly higher amounts of interferon (IFN)-γ in response to chrome stimulation than lymphocytes from nonallergic individuals [8] .…”

mentioning

confidence: 99%

Luminex LiquiChip System for the Evaluation of Cytokine Levels in Patients with Contact Dermatitis to Potassium Dichromate

Akerman

Klein²,

Israeli³

et al. 2016

Dermatology

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Background: Allergic contact dermatitis (ACD) is associated with increased production of cytokines. The patch test is the “gold-standard” diagnostic method, but it poses a risk of false results. Objective: To evaluate a novel laboratory technique, the Luminex LiquiChip, which simultaneously measures blood levels of multiple cytokines, as a diagnostic tool in patients with chrome-induced ACD. Methods: The study group included 20 patients with ACD and relevant patch test results for potassium dichromate and 19 patients with ACD for nickel or fragrance as control. Peripheral blood mononuclear cells (PBMCs) were cultured in the presence and absence of potassium dichromate. The Luminex LiquiChip was used to measure levels of the following cytokines: granulocyte-macrophage colony-stimulating factor, interleukin (IL)-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, interferon-γ, and tumor necrosis factor (TNF)-α. Results: Potassium dichromate-stimulated PBMCs secreted significantly higher amounts of all cytokines except TNF-α than nonstimulated PBMCs. PBMCs from patients with ACD to chromium secreted significantly higher amounts of all cytokines tested, except IL-4, compared to PBMCs from patients with ACD to nickel or fragrance. Conclusions: Potassium dichromate stimulates the production of both Th1- and Th2-type cytokines in patients with chrome allergy. The Luminex LiquiChip is a promising in vitro method and may serve as a diagnostic tool for ACD.

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“…These researchers performed 24-hour cultures stimulated with potassium dichromate and phytohemagglutinin, a non-specific activator of T cells supposedly used to initiate and expand the reaction. 17,18 …”

Section: Discussionmentioning

confidence: 99%

Cytokine detection for the diagnosis of chromium allergy

Martins

Reis

2013

An. Bras. Dermatol.

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BACKGROUNDPatch testing remains the gold standard method for the identification of the etiologic agent of allergic contact dermatitis. However, it is a subjective, time-consuming exam whose technique demands special care and which presents some contraindications, which hamper its use. In a recent study, we showed that the proliferation assay can suitably replace patch testing for the diagnosis of chromium allergy, which had been previously demonstrated only for nickel allergy. In this study, we try to refine the method by reducing the incubation period of cultures for lymphocyte proliferation assays in response to chromium. OBJECTIVEDevelop an alternative or complementary diagnostic test for chromium allergic contact dermatitis.METHODSWe compared the production of 9 cytokines (IFN-γ, IL-2, IL-4, IL-5, IL-10, IL-12, IL-13, IL-17 and RANTES) between 18 chromium-allergic patients and 19 controls. RESULTSChromium increased the production of IFN-y, IL-5, IL-2 and IL-13 in allergic patients, but only IL-2 and especially IL-13 helped discriminate allergic patients from controls. The sensitivity, specificity and accuracy found with IL-13 were about 80%. CONCLUSIONS IL-13 and IL-2 detection may be used to diagnose chromium allergy in 2-day cultures. However, in general, the 6-day cultures seem to be superior for this purpose.

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Use of in vitro release of interferon‐γ in the diagnosis of contact allergy to potassium dichromate – a controlled study

Cited by 12 publications

References 13 publications

Improved detection of allergen‐specific T‐cell responses in allergic contact dermatitis through the addition of ‘cytokine cocktails’

Improved detection of allergen‐specific T‐cell responses in allergic contact dermatitis through the addition of ‘cytokine cocktails’

Luminex LiquiChip System for the Evaluation of Cytokine Levels in Patients with Contact Dermatitis to Potassium Dichromate

Cytokine detection for the diagnosis of chromium allergy

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