Use of hydrogen peroxide to assess the sperm susceptibility to oxidative stress in subjects presenting a normal semen profile

Misro, M. M.; Choudhury, Lubna; Upreti, K. K.; Gautam, D. K.; Chaki, Sankar P.; Mahajan, Aarti; Babbar, Rashmi

doi:10.1046/j.0105-6263.2003.00451.x

Cited by 36 publications

(27 citation statements)

References 22 publications

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“…(21) Such ROS may react with the high concentrations of polyunsaturated fatty acids in the sperm plasma membrane leading to a loss of membrane fluidity and integrity. (79) Therefore, sperm carrying oxidised DNA should be more vulnerable to the further attack of free radicals, being discarded in long female tracts. Reduced fecundity of sperm with oxidized DNA (19) is likely due to detrimental ROS effects on the motility of spermatozoa, peroxidation of membrane lipids leading to compromised sperm-ovum fusion and decreased chromatin quality.…”

Section: Physiological Postcopulatory Mechanismsmentioning

confidence: 99%

Avoiding bad genes: oxidatively damaged DNA in germ line and mate choice

2008

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August Weismann proposed that genetic changes in somatic cells cannot pass to germ cells and hence to next generations. Nevertheless, evidence is accumulating that some environmental effects can promote heritable changes in the DNA of germ cells, which implies that some somatic influence on germ line is possible. This influence is mostly detrimental and related to the presence of oxidative stress, which induces mutations and epigenetic changes. This effect should be stronger in males due to the particular characteristics of sperm. Here, we propose the hypothesis that females are able to avoid males with oxidatively damaged DNA in the germ line by using oxidative‐dependent (pre‐ and post‐mating) signals. This new hypothesis may shed light on unsolved questions in evolutionary biology, such as the benefits of polyandry, the lek paradox, or the role of sexual selection on the evolution of aging. BioEssays 30:1212–1219, 2008. © 2008 Wiley Periodicals, Inc.

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Section: Physiological Postcopulatory Mechanismsmentioning

confidence: 99%

Avoiding bad genes: oxidatively damaged DNA in germ line and mate choice

2008

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“…The SOD [6,8,18] and catalase [17,24] in seminal plasma are produced by the testis, epididymis, accessory reproductive organs, and sperm, and they are able to maintain sperm motility for a long time [6,8]. Seminal plasma SOD has been reported to have the same effect on canine sperm [5].…”

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confidence: 90%

Superoxide Dismutase and Catalase Activities in the Seminal Plasma of Normozoospermic and Asthenozoospermic Beagles

Kawakami

Takemura

Sakuma

et al. 2007

The Journal of Veterinary Medical Science

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ABSTRACT. We measured the blood plasma testosterone (T) levels and superoxide dismutase (SOD) and catalase activities in the seminal plasma of the ejaculates of 5 normal (2-5 years old) and 5 asthenozoospermic (AZ-) (3-5 years old) Beagles. Sperm ejaculated by AZdogs was incubated for 3 hr in Eagle's MEM only (controls) or Eagle's MEM containing 100 units/ml of SOD or catalase. Sperm motility was examined during incubation. The mean (± SE) plasma T level of the AZ-dogs (1.2 ± 0.2 ng/ml) was significantly lower than in the normal dogs (2.5 ± 0.2 ng/ml) (P<0.005). The mean (± SE) seminal plasma SOD and catalase activities (18.8 ± 1.9 and 0.5 ± 0.1 unit/g protein, respectively) were significantly lower in the AZ-dogs than in the normal dog (43.3 ± 2.5 and 2.2 ± 0.4 unit/g protein, respectively) (P<0.001 and 0.01, respectively). The motility of sperm incubated in Eagle's MEM containing SOD or catalase was significantly higher than that of control sperm incubated in only Eagle's MEM after 2 or 3 hr of incubation (P<0.05). The results of this study indicate that poor T secretion by the testes and low antioxidant enzyme activities are related to AZ in the dog. KEY WORDS: canine, catalase, seminal plasma, SOD.J. Vet. Med. Sci. 69(2): 133-136, 2007 Seminal plasma is known to contain reactive oxygen species (ROS) produced by testicular tissue [18] and sperm [2,20], and elevated seminal plasma ROS concentrations in both humans [1,16] and dogs [22] have been reported to be a cause of oligozoospermia, asthenozoospermia, and teratozoospermia. Low sperm motility and morphologically abnormal sperm occur as a result of sperm plasma membrane dysfunction caused by ROS [7].Superoxide dismutase (SOD) [2,6,9] and catalase [6,19,24] are the main antioxidant enzymes in seminal plasma that prevent increases in ROS concentration in seminal plasma and protect the sperm against damage and oxidative stress caused by ROS. The SOD [6,8,18] and catalase [17,24] in seminal plasma are produced by the testis, epididymis, accessory reproductive organs, and sperm, and they are able to maintain sperm motility for a long time [6,8]. Seminal plasma SOD has been reported to have the same effect on canine sperm [5]. However, the cause of spermatogenic arrest in the dog is unknown [12]. In the present study, we examined the interaction between peripheral blood plasma testosterone (T) levels and the activities of seminal plasma SOD and catalase in the dog. Based on the results of this study, we suggested some causes for spermatogenic dysfunction in the dog. MATERIALS AND METHODS Animals:Ten male Beagles aged 2-5 years were used in this study. They were cared for in our university and housed in pens with ample runs. Commercial dry dog food was provided twice a day, and the dogs were given free access to water. All animals were maintained according to the guidelines of the Animal Care and Use Committee of the Nippon Veterinary and Life Science University.The semen quality of the dogs was examined 3 times at one-week intervals. Five of the dogs (3-5 years...

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“…On the other hand, in vitro exposure to reactive oxygen species (ROS) resulted in membrane damage [52] that is linked with an increase in ROS induced DNA oxidation that renders the sperm unable to fertilize the oocyte or produce a viable pregnancy [24,29,38,41,53]. The resulting increase in the kinetics of decondensation (accelerated decondensation), as well as an increase in the recondensation kinetics shown in Figs.…”

Section: Discussionmentioning

confidence: 99%

Evaluating a novel panel of sperm function tests for utility in predicting intracytoplasmic sperm injection (ICSI) outcome

Brown

Merryman²,

Rivnay

et al. 2013

J Assist Reprod Genet

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Purpose The objective of this study was to evaluate a panel of three sperm function tests; tests known to assess different aspects of sperm functionality and genomic integrity, the: 1) Sperm DNA Accelerated Decondensation (SDAD TM ) Test, 2) Sperm DNA Decondensation (SDD TM ) Test, and 3) Sperm Penetration Assay (SPA), determining if positive and negative test scores correlated with failed and successful ICSI outcomes, respectfully. Methods A prospective, double blinded, cohort study was performed. One study sample (ejaculated semen) was collected by each of the 60 male partners of the 60 couples enrolled in the study; males whose female partners were found to have no major female factor issues. The sperm from each male was analyzed in the SPA, and SDAD and SDD Tests, and used for ICSI (1 ICSI cycle per couple). Results The ICSI cycle pregnancy rate for this study was 50 %, with a delivery rate040 % (n060 ICSI cycles). The SPA and SDD Test scores did not significantly predict ICSI outcome when used as stand-alone tests (p>>0.05). However, when the SPA and SDD Test scores were used together, ICSI outcomes for a subgroup of 10 (16.7 %) males, were significantly predicted (p00.03), with 1 live birth, and 9 negatives where the transferred embryos did not implant. In total, 38.4 % of the couples in this study were found to have a very poor chance for a successful ICSI cycle. Conclusion SDAD Test scores alone, and SPA and SDD Test scores used together, significantly predicted failed ICSI outcomes. This indicates that the scores obtained when analyzing patients' sperm using a panel of sperm function tests; specifically, the SPA, and SDAD and SDD Tests, can be used to identify infertile couples who should not be directed to ICSI.

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Use of hydrogen peroxide to assess the sperm susceptibility to oxidative stress in subjects presenting a normal semen profile

Cited by 36 publications

References 22 publications

Avoiding bad genes: oxidatively damaged DNA in germ line and mate choice

Avoiding bad genes: oxidatively damaged DNA in germ line and mate choice

Superoxide Dismutase and Catalase Activities in the Seminal Plasma of Normozoospermic and Asthenozoospermic Beagles

Evaluating a novel panel of sperm function tests for utility in predicting intracytoplasmic sperm injection (ICSI) outcome

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