Use of Hydrogen Peroxide and Formalin Treatments during Incubation of Landlocked Fall Chinook Salmon Eyed Eggs

Barnes, Michael E.; Stephenson, Hans; Gabel, Mark

doi:10.1577/1548-8454(2003)65<151:uohpaf>2.0.co;2

Cited by 37 publications

(31 citation statements)

References 21 publications

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“…Fall Chinook salmon Oncorhynchus tshawytscha eggs have only been used in two previous studies. Waterstrat and Marking (1995) observed similar fungal clumping in fall Chinook salmon eggs treated with hydrogen peroxide at 500 or 1,000 mg/L, or formalin at 1,000 mg/L; Barnes et al (2003), however, used 700 mg/L hydrogen peroxide on eyed eggs through fry hatch. The use of hydrogen peroxide concentrations greater than 1,000 mg/L, as well as treatment regimes incorporating reduced treatment levels during possible embryonic sensitive changes, have not been evaluated for fall Chinook salmon.…”

Section: Please Scroll Down For Articlementioning

confidence: 96%

Use of Hydrogen Peroxide during Incubation of Landlocked Fall Chinook Salmon Eggs in Vertical‐Flow Incubators

Barnes

Gaikowski

2004

N American J Aquac

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Six different hydrogen peroxide treatment regimes were evaluated in a series of three trials with landlocked fall Chinook salmon Oncorhynchus tshawytscha eggs incubated in vertical‐flow incubators. Six daily 15‐min hydrogen peroxide treatment regimes (1,000 mg/L; 1,000 mg/L with a decrease to 500 mg/L during estimated blastopore formation; 2,000 mg/L; 2,000 mg/L with a decrease to 500 mg/L during estimated blastopore formation; 2,500 mg/L; and 2,500 mg/L with a decrease to 500 mg/L during estimated blastopore formation) were compared with daily 15‐min treatments of 1,667 mg/L of formalin. Mortality at egg eye‐up and fry hatch and from eye‐up to hatch was significantly greater in eggs receiving the 2,500‐mg/L hydrogen peroxide treatments throughout incubation and in those receiving 2,500 mg/L hydrogen peroxide with a decrease to 500 mg/L during blastopore formation than in either of the 1,000‐mg/L hydrogen peroxide treatment regimes or the formalin‐treated eggs in the first trial. No significant differences in mortality were observed among any of the treatments in the subsequent two trials with maximum hydrogen peroxide concentrations of 2,000 mg/L. Fungal infestations were observed primarily in the incubation trays treated at either of the 1,000‐mg/L hydrogen peroxide regimens, as well as in those trays whose treatment concentrations were dropped to 500 mg/L during blastopore formation. Infestations were not observed in any of the formalin‐treated trays. If minor fungal infestation is acceptable, then daily hydrogen peroxide treatments of 1,000 mg/L for 15 min would probably provide adequate fungal control compared with formalin usage.

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Section: Please Scroll Down For Articlementioning

confidence: 96%

Use of Hydrogen Peroxide during Incubation of Landlocked Fall Chinook Salmon Eggs in Vertical‐Flow Incubators

Barnes

Gaikowski

2004

N American J Aquac

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“…These repeated disinfections were performed daily (Barnes et al . , , ,b, ; Small & Chatakondi ), twice a day (Barnes et al . ; Khodabandeh & Abtahi ), 3 or 4 times a day (Small & Chatakondi ), every other day (Bailey & Jeffrey ; Rach et al .…”

Section: Evaluation Criteria For a Successful Disinfectionmentioning

confidence: 99%

Disinfection of teleost eggs: a review

Swaef

Broeck

Dierckens

et al. 2015

Reviews in Aquaculture

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In aquaculture hatcheries, egg disinfection is commonly employed as a mortality mitigation and disease management tool. In addition, disinfection protocols are utilized in research facilities, as a means to create axenic and gnotobiotic larval models. For the latter, a complete sterilization of the eggs is warranted. Multiple research groups have tested various disinfectants in fish eggs adopting different protocols and a range of parameters to evaluate their efficacy and safety. However, there is a clear lack of a critical review listing these different studies and stressing the advantages and shortcomings of the protocols and adopted disinfectants. This review provides a commented survey of the findings/experiments on the most frequently used and upcoming disinfecting products, the protocols in which they are included and their purposes with emphasis on decreasing bacterial and/or fungal load, underscoring the benefits and the limitations of each product. Supplemented with a critical note, this review assists both aquaculturists and researchers in making an informed choice. Moreover, it will help stakeholders to identify the gaps in knowledge where future research on egg disinfection is needed.

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“…The most used alternative treatments currently available are formalin (however, its use is likely to be banned soon due to adverse environmental impacts (Bruno, van West & Beakes ; Liu et al . )), bronopol, sodium chloride and hydrogen peroxide (Waterstrat & Marking ; Branson ; Barnes, Stephenson & Gabel ; Magaraggia et al . ).…”

Section: Introductionmentioning

confidence: 99%

A fluorescence‐based assay for in vitro screening of Saprolegnia inhibitors

Ali

Skaar

2017

Journal of Fish Diseases

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The incidence of fish pathogenic oomycetes, Saprolegnia, has increased significantly in aquaculture since the ban of malachite green. For the efficient characterization of anti-Saprolegnia therapeutics, simple accurate methods are required. However, the current screening methods are limited by time, and none of them are confirming the viability of treated spores or hyphae. In this study, a modified fluorescence-based assay for the in vitro screening of Saprolegnia inhibitors has been developed. This method involves the use of FUN-1 viability dye combined with calcofluor white M2R, and is based on the formation of orange-red cylindrical intravacuolar structures (CIVS) in metabolically active spores, hyphae and biofilms. Heat-killed and bronopol-treated Saprolegnia spores, hyphae and biofilms exhibited diffuse bright green fluorescence which confirms complete loss of viability. For boric acid-treated spores, no germination was observed. However, tiny CIVS were observed in 50% of treated spores which indicated reduction in their viability. Our results proved that FUN-1 dye is an efficient tool to distinguish between live and dead Saprolegnia spores, hyphae and biofilms and to monitor the change in Saprolegnia viability during qualitative evaluation of potential anti-Saprolegnia compounds.

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Use of Hydrogen Peroxide and Formalin Treatments during Incubation of Landlocked Fall Chinook Salmon Eyed Eggs

Cited by 37 publications

References 21 publications

Use of Hydrogen Peroxide during Incubation of Landlocked Fall Chinook Salmon Eggs in Vertical‐Flow Incubators

Use of Hydrogen Peroxide during Incubation of Landlocked Fall Chinook Salmon Eggs in Vertical‐Flow Incubators

Disinfection of teleost eggs: a review

A fluorescence‐based assay for in vitro screening of Saprolegnia inhibitors

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