Use of hybridoma-resistant variant cell lines to study H-2D b/FMR-specific cytotoxic T cells

Abstract

An H-2Db heterozygous tumor cell line and a variant subclone bearing a mutant gene product were used to analyze the H-2Db specificity of cytotoxic T lymphocytes (CTL) generated during a Moloney murine sarcoma virus (MSV) infection. When the mutant cells were used as targets for MSV-specific CTL, the amount of cell lysis, compared with that seen with the nonmutant parental cells, was drastically decreased. However, cells of the mutant clones remained susceptible to allogeneic CTL specific for the nonmutant H-2Db molecule. The mutant cells also did not differ from the parent cells in their level of viral antigen expression. Biochemically the parental and mutant molecules were similar but not identical. The data indicate that minor alterations of the H-2 antigens caused by somatic mutation may prevent virus-infected cells from being recognized as targets by CTL.

Somatic H‐2Kk variants reveal nonidentity of serological and cytotoxic T cell‐defined Kk determinants

“…Somatic H-2 variants expressing structurally modified H-2 molecules, which differ from wild type in determinants defined by monoclonal antibodies (mAb) [4, H-2 determinants recognized by CTL. Chapdalaine et al [6] first reported about an H-2 mutant of a moloney murine sarcoma virus (MSV)-induced cell line of (BALB.B x BALB/c)F1 origin (H-2Kb'd,Db'd), which was isolated by cytotoxic selection with a Db-specific mAb. This H-2 variant was hardly lysed by H-2b-restricted, Friend, Moloney, Rauscher virus (FMR)specific CTL, whereas alloreactive H-2b-specific CTL did not discriminate it from wild type cells.…”

Selection of cell lines resistant to anti-transferrin receptor antibody: evidence for a mutation in transferrin receptor.

“…5 VOL. 4,1984 parental cells, rather than 10-1o to 10-8, as would be expected for a recessive alteration requiring two mutation steps in a diploid cell (2,14).…”