Use of human keratinocytes cultured on fibrin glue in the treatment of burn wounds

Ronfard, Vincent; Broly, H.; Mitchell, Valerie A.; Galizia, J.P.; Hochart, D.; Chambon, E.; Pellerin, P; Huart, J. J.

doi:10.1016/0305-4179(91)90099-3

Cited by 124 publications

(48 citation statements)

References 11 publications

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“…Ronfard et al (1991) used keratinocytes, which were cultured for a short time on fibrin gel, as a treatment for thermal burns and obtained good clinical results. Kaiser et al (1994) cultured autologous keratinocytes with fibrin and developed them into an epidermal sheet, applied it in the treatment of thermal burns, and obtained a high take rate.…”

confidence: 99%

Fibrin Stimulates the Proliferation of Human Keratinocytes through the Autocrine Mechanism of Transforming Growth Factor-.ALPHA. and Epidermal Growth Factor Receptor

Yamamoto

Yanaga²,

Nishina³

et al. 2005

Tohoku J. Exp. Med.

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In the field of dermatology and plastic and reconstructive surgery, fibrin gel is regarded as a material that promotes wound healing. To test the hypothesis that fibrin may promote the growth of the epidermis, we examined its effects on the proliferation of cultured keratinocytes. Human keratinocytes were cultivated in fibrincoated wells, and the cell numbers and transforming growth factor (TGF)-α , secreted into the cultured medium, were measured. We also assessed the capacity of epidermal growth factor receptor (EGF-R) that is responsible for all known actions of TGF-α and epidermal growth factor. The keratinocytes increased dramatically in their number, and the TGF-α secretion and the binding capacity of EGF-R were also increased dramatically in the presence of fibrin. These findings suggest that fibrin supports the proliferation of keratinocytes in an autocrine fashion via EGF-R; namely, fibrin stimulates keratinocytes to secrete TGF-α, which in turn increases cell proliferation and EGF-R capacity. We propose that fibrin can support the wound healing process of the epidermis via the TGF-α /EGF-R pathway.fibrin; keratinocytes; TGF-α ; EGF-R; wound healing © 2005 Tohoku University Medical PressFibrin gel is commonly used in clinical applications, as it had been originally developed as a biological glue for wound closure and tissue fixation. Therefore, it has mainly been used to stop bleeding and the loss of bodily fluid. Recently in the fields of dermatology and plastic and reconstructive surgery, fibrin gel began to be regarded as a material that promotes the wound-healing process and results in a good take rate for epidermis grafts and in faster wound healing.There are several clinical reports on the use of fibrin gel as a wound healing promoter. Ronfard

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confidence: 99%

Fibrin Stimulates the Proliferation of Human Keratinocytes through the Autocrine Mechanism of Transforming Growth Factor-.ALPHA. and Epidermal Growth Factor Receptor

Yamamoto

Yanaga²,

Nishina³

et al. 2005

Tohoku J. Exp. Med.

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“…Thus, the use of fibrin glue may favor the epithelization of open wounds. 17,21 In this study, there was no secondary infection in any of the cases although all the wounds received garamycin cream. It was noticeable that in the cases where fibrin glue was used, the antibiotic cream remained completely isolated, without becoming mixed up with the glue.…”

Section: Second Intention Healingmentioning

confidence: 51%

“…4,5,7,9,11,[14][15][16][17][18]21,22 The continuing evolution in the preparation and use of fibrin glue is emphasized in the literature.…”

Section: Discussionmentioning

confidence: 99%

Use of autologous fibrin glue in dermatologic surgery: application of skin graft and second intention healing

1998

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Objective:To evaluate the efficiency of biological sealant, an autologous fibrin glue, in dermatological surgery. Design: Randomized clinical trial. Setting: The Dermatology Service of Hospital das Clínicas, Universidade de Campinas (UNICAMP), referral center. Patients: 14 patients with malign epithelial cutaneoos tumors participated in the evaluation, each having two tumors, generally facial and symmetrical, in order to perform a comparative evaluation on the same individual. Procedures: The glue was prepared beforehand with a sample of autologous blood. Surgical extirpation of the tumor was followed by grafts or second intention healing. Outcomes: The efficiency of the sealant was then evaluated in relation to hemostasis, adhesion, surgical time and evolution of the granulation tissue, clinically and histologically. Results: Immediate hemostasis and graft adhesion, with a significant reduction of surgical time, and in the open wounds there was immediate hemostasis and a clinical increase in granulation tissue, but with no histological differences among the groups on the 7 th day. Conclusion: It is an adjuvant resource in skin cancer surgery.Uniterms: Fibrin glue. Wound healing. Skin cancer. Dermatologic surgery.

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“…Th e utilization of fi brin gel in skin engineering started in 1988, with fi brin glue fi xation of cultivated keratinocytes to the wound bed 14 . In several studies, burned patients were successfully treated with keratinocytes in fi brin gel [15][16][17] . Fibrin has also been used as a scaff old for cultivating dermal cell components.…”

Section: Introductionmentioning

confidence: 99%

Fibrin Gel as a Scaffold for Skin Substitute – Production and Clinical Experience

Kljenak

Trcin²,

Bujić³

et al. 2016

ACC

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SUMMARY -Th e purpose of this study was to create a fi brin-based human skin substitute in vitro with epidermal and dermal component and to assess its healing potential in deep partial and full thickness burns. Fibrin scaff olds were prepared from commercial fi brin glue kits. Human fi broblasts were cultured in fi brin gel. Human keratinocytes were seeded on the top of the gel. Viability of cells was determined fl uorimetrically. Scanning electron microscope and immunocytochemistry analysis of cultured cells were performed. After hydrosurgical preparation of deep burn necrotic tissue, wound bed was prepared for skin substitutes. Progress of healing was documented using visual estimation and photos. Scanning electron microscope images showed good cell attachment and colony spreading of keratinocytes and fi broblasts on fi brin scaff old. Immunofl uorescent staining of cell cultures on fi brin scaff old showed expression of vimentin, a marker of fi broblast cells, cytokeratin 19, a marker of epithelial stem cells, as well as involucrin, a marker of diff erentiated keratinocytes. Clinical results clearly showed that appearance of the skin did not diff er signifi cantly from the areas of transplanted skin using split-thickness skin graft techniques. In conclusion, using these fi brin-cultured autografts on massive full-thickness burn resulted in good healing.

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Use of human keratinocytes cultured on fibrin glue in the treatment of burn wounds

Cited by 124 publications

References 11 publications

Fibrin Stimulates the Proliferation of Human Keratinocytes through the Autocrine Mechanism of Transforming Growth Factor-.ALPHA. and Epidermal Growth Factor Receptor

Fibrin Stimulates the Proliferation of Human Keratinocytes through the Autocrine Mechanism of Transforming Growth Factor-.ALPHA. and Epidermal Growth Factor Receptor

Use of autologous fibrin glue in dermatologic surgery: application of skin graft and second intention healing

Fibrin Gel as a Scaffold for Skin Substitute – Production and Clinical Experience

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