Use of HPTLC with non-aqueous binary mobile phases for determination of selected porphyrins

Analysis of Urinary Porphyrins by High Performance Liquid Chromatography-Electrospray Ionization Mass Spectrometry

“…In the early years, solvent fractionation methods were usually performed, [19,20] which were laborious and imprecise in the separation of porphyrins. More recently, a number of separation methods have been developed and applied for the analysis of porphyrins in biological samples, including gas chromatography (GC), [21] flow-injection analysis (FIA), [22] thin-layer chromatography (TLC), [23,24] high performance liquid chromatography (HPLC), [25][26][27] and capillary electrophoresis (CE). [28,29] HPLC has obtained considerable attention for the porphyrin separation [30] with various detection techniques including immunoassay, [31] inductive coupled plasma-atomic emission spectrum (ICP-AES), [32] electrochemical mode, [33,34] and the conventional ultraviolet visible (UV-Vis) detector.…”