Use of HPLC in the Determination of the Molar Absorptivity of 4′-Geranyloxyferulic Acid and Boropinic Acid

Locatelli, Marcello; Carlucci, Giuseppe; Genovese, Salvatore; Curini, Massimo; Epifano, Francesco

doi:10.1007/s10337-011-1979-5

Cited by 13 publications

(10 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Lambert-Beer's law is valid for a DAD detector, which is a spectrophotometer submitted to a dynamic and continuous HPLC flow, operating on chromatographically separated molecules. As proposed by Locatelli et al [35], (3) can be used when is calculated as the slope of a straight-line regression built using the injected mass and the corresponding areas recorded by the DAD detector as follows:…”

Section: Calculation Of the Molar Absorptivity Coefficient By Hplcmentioning

confidence: 99%

Measurement of Capsaicinoids in Chiltepin Hot Pepper: A Comparison Study between Spectrophotometric Method and High Performance Liquid Chromatography Analysis

González‐Zamora

Sierra-Campos

Pérez‐Morales

et al. 2015

Journal of Chemistry

View full text Add to dashboard Cite

Direct spectrophotometric determination of capsaicinoids content in Chiltepin pepper was investigated as a possible alternative to HPLC analysis. Capsaicinoids were extracted from Chiltepin in red ripe and green fruit with acetonitrile and evaluated quantitatively using the HPLC method with capsaicin and dihydrocapsaicin standards. Three samples of different treatment were analyzed for their capsaicinoids content successfully by these methods. HPLC-DAD revealed that capsaicin, dihydrocapsaicin, and nordihydrocapsaicin comprised up to 98% of total capsaicinoids detected. The absorbance of the diluted samples was read on a spectrophotometer at 215–300 nm and monitored at 280 nm. We report herein the comparison between traditional UV assays and HPLC-DAD methods for the determination of the molar absorptivity coefficient of capsaicin (ε280=3,410andε280=3,720 M−1 cm−1) and dihydrocapsaicin (ε280=4,175andε280=4,350 M−1 cm−1), respectively. Statistical comparisons were performed using the regression analyses (ordinary linear regression and Deming regression) and Bland-Altman analysis. Comparative data for pungency was determined spectrophotometrically and by HPLC on samples ranging from 29.55 to 129 mg/g with a correlation of 0.91. These results indicate that the two methods significantly agree. The described spectrophotometric method can be routinely used for total capsaicinoids analysis and quality control in agricultural and pharmaceutical analysis.

show abstract

Section: Calculation Of the Molar Absorptivity Coefficient By Hplcmentioning

confidence: 99%

Measurement of Capsaicinoids in Chiltepin Hot Pepper: A Comparison Study between Spectrophotometric Method and High Performance Liquid Chromatography Analysis

González‐Zamora

Sierra-Campos

Pérez‐Morales

et al. 2015

Journal of Chemistry

View full text Add to dashboard Cite

show abstract

“…The concentrations of vitamins A and E in the stock solutions were determined by two methods. In the first method we used dynamic flow HPLC coupled with diode array detector [ 18 – 20 ]. The second method is the static UV/VIS spectrophotometer using Beer’s Law [ 21 – 27 ] with purity corrections for impurity absorbed at the same wavelength by liquid chromatography.…”

Section: Resultsmentioning

confidence: 99%

Development of two reference materials for all trans-retinol, retinyl palmitate, α- and γ-tocopherol in milk powder and infant formula

Shehata

Rizk

Farag

et al. 2015

Journal of Food and Drug Analysis

View full text Add to dashboard Cite

Vitamins are important food constituents that can be present in almost every foodstuff. Food quality and safety depends on food surveillance by reliable quantitative analysis enabled by appropriate quality control. Certified matrix reference materials are versatile tools to support quality assurance and control. However, in the case of vitamins, which are important in various foods, there is a lack of matrix reference materials. Two certified reference materials for the determination of all-trans-retinol, retinyl palmitate, and α- and γ-tocopherol in milk powder and infant formula have been developed by the National Institute of Standards, Egypt. This article presents the preparation, characterization, homogeneity, and stability testing as well as statistical treatment of data and certified value assignment. The assignment of the certified values and associated uncertainties in the prepared natural-matrix reference materials were based on the widely used approach of combining data from independent and reliable analytical methods.

show abstract

“…Within each HPLC trace, the area under the curve for the product peptide was extracted and calculated using a custom R-script. To calculate the amount of converted product as a result of the enzymatic reaction, we employed a modified version of Lambert-Beer's law, [114] with…”

Section: Determination Of Degree Of Substitution (Nmr)mentioning

confidence: 99%

Enzymatically Crosslinked Collagen as a Versatile Matrix for In Vitro and In Vivo Co‐Engineering of Blood and Lymphatic Vasculature

et al. 2023

View full text Add to dashboard Cite

are missing extensive pericyte coverage. In addition, lymphatics exhibit an irregularly shaped or collapsed lumen lacking red blood cells (RBCs). [1][2][3] Lymphatic vascular structures are generally classified as either capillaries (diameter 10-60 µm) or collecting lymphatic vessels (diameter 50-200 µm). [4] Similarly, blood vessels can be categorized into either capillaries (4-10 µm) or larger structures such as arterioles and venules (10-100 µm). [5] Despite their structural differences, the blood and lymphatic vasculature are closely interconnected on a functional level to ensure nutritional supply of cells and tissue homeostasis. [6][7][8] The absence of a functional vascular network in vitro is one of the main drawbacks of many bioengineered tissues and a frequent cause of necrosis in vivo. [9] Whereas most previous studies focused on the blood vasculature, recent discoveries have brought increased attention to the role of lymphatics and their role in tissue regeneration. [7,8] Yet only a few studies have so far attempted to co-engineer blood and lymphatic microcapillaries. Marino et al. described a simple co-culturing system of blood endothelial cells (BECs) and lymphatic endothelial cells (LECs) based on native fibrin and collagen hydrogels. [10] The resulting microcapillaries remained in their early developmental stages, with phenotypes exhibiting mostly short capillary fragments Adequate vascularization is required for the successful translation of many in vitro engineered tissues. This study presents a novel collagen derivative that harbors multiple recognition peptides for orthogonal enzymatic crosslinking based on sortase A (SrtA) and Factor XIII (FXIII). SrtA-mediated crosslinking enables the rapid co-engineering of human blood and lymphatic microcapillaries and mesoscale capillaries in bulk hydrogels. Whereas tuning of gel stiffness determines the extent of neovascularization, the relative number of blood and lymphatic capillaries recapitulates the ratio of blood and lymphatic endothelial cells originally seeded into the hydrogel. Bioengineered capillaries readily form luminal structures and exhibit typical maturation markers both in vitro and in vivo. The secondary crosslinking enzyme Factor XIII is used for in situ tethering of the VEGF mimetic QK peptide to collagen. This approach supports the formation of blood and lymphatic capillaries in the absence of exogenous VEGF. Orthogonal enzymatic crosslinking is further used to bioengineer hydrogels with spatially defined polymer compositions with pro-and anti-angiogenic properties. Finally, macroporous scaffolds based on secondary crosslinking of microgels enable vascularization independent from supporting fibroblasts. Overall, this work demonstrates for the first time the co-engineering of mature micro-and meso-sized blood and lymphatic capillaries using a highly versatile collagen derivative.

show abstract

Use of HPLC in the Determination of the Molar Absorptivity of 4′-Geranyloxyferulic Acid and Boropinic Acid

Cited by 13 publications

References 17 publications

Measurement of Capsaicinoids in Chiltepin Hot Pepper: A Comparison Study between Spectrophotometric Method and High Performance Liquid Chromatography Analysis

Measurement of Capsaicinoids in Chiltepin Hot Pepper: A Comparison Study between Spectrophotometric Method and High Performance Liquid Chromatography Analysis

Development of two reference materials for all trans-retinol, retinyl palmitate, α- and γ-tocopherol in milk powder and infant formula

Enzymatically Crosslinked Collagen as a Versatile Matrix for In Vitro and In Vivo Co‐Engineering of Blood and Lymphatic Vasculature

Contact Info

Product

Resources

About