Use of HPLC for increasing the molar specific activities of tritium‐labelled nucleosides

Abstract

The p o s s i b i l i t y of c h r o m a t o g r a p h i c s e p a r a t i o n of d i f f e r e n t i s o t o p i c f o r m s of t r i t i a t e d n u c l e o s i d e s h a s been d e m o n s t , r a t e d . Using a r e v e r s e d -p h a s e column i n 3-5% s o l u t . i o n s of e t , h a n o l i n c h r o m a t m g r a p h i c columns f see, f o r e:xampl.e [ 1-31). It. seems a p p r o p r i a t e t.o u s e t h e isot.ope e f f e c t s for o b t a i n i n g p r e p a r a t . i o n s w i t h h i g h molar s p e c i f i c a c t i . v i t i e s on t h e stage of f i n a l c h r o m a t o g r a p h i c p u r i f i c a t i o n . The abject. of t h e p r e s e n t , work w a s an i n v e s t i g a t i o n of u s i n g HPLC f o r f r a c t , i o n a t i o n of t r i t i u m -l a b e l l e d nucleos.ides a c c o r d i n g t o i t s m o l a r s p e c i f i c a c t i v i t y v a l u e s .